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Cited by 89Spectroscopy Techniques in Biomedical and Chemical ResearchAdvanced Fluorescence Microscopy TechniquesSpectroscopy and Quantum Chemical Studies

Shedding new light on lipid functions with CARS and SRS microscopy

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Yu, Yong, Ramachandran, Prasanna V., Wang, Meng C.·2014·Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
DOI 10.1016/j.bbalip.2014.02.003PMC PMC4285713
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Abstract

Modern optical microscopy has granted biomedical scientists unprecedented access to the inner workings of a cell, and revolutionized our understanding of the molecular mechanisms underlying physiological and disease states. In spite of these advances, however, visualization of certain classes of molecules (e.g. lipids) at the sub-cellular level has remained elusive. Recently developed chemical imaging modalities - Coherent Anti-Stokes Raman Scattering (CARS) microscopy and Stimulated Raman Scattering (SRS) microscopy - have helped bridge this gap. By selectively imaging the vibration of a specific chemical group, these non-invasive techniques allow high-resolution imaging of individual molecules in vivo, and circumvent the need for potentially perturbative extrinsic labels. These tools have already been applied to the study of fat metabolism, helping uncover novel regulators of lipid storage. Here we review the underlying principle of CARS and SRS microscopy, and discuss the advantages and caveats of each technique. We also review recent applications of these tools in the study of lipids as well as other biomolecules, and conclude with a brief guide for interested researchers to build and use CARS/SRS systems for their own research. This article is part of a Special Issue entitled Tools to study lipid functions.

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  • The optimized microglia isolation strategy uses mechanical tissue homogenization instead of enzymatic digestion, which keeps microglia largely in a non-activated state and prevents unwanted bias towards an activated pro-inflammatory signature.

    “Of note, we used an optimized microglia isolation strategy that uses mechanical tissue homogenization instead of enzymatic digestion, which keeps microglia largely in a non-activated state and therefore prevents unwanted bias towards an activated pro-inflammatory signature19.”

    From Lipid-droplet-accumulating microglia represent a dysfunctional and proinflammatory state in the aging brain (2020) · cites this paper as [19]

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Cited by

Sorted by citations

Authors
Yu, Y., Ramachandran, P. V., & Wang, M. C.
Affiliations
Baylor College of Medicine (US)
Corresponding author
Wang, Meng C.
ORCID
Venue
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
Publisher
Elsevier BV
Published
2014-08
Volume
1841
Issue
8
Pages
1120-1129
ISSN
1388-1981 (ISSN-L)
DOI
10.1016/j.bbalip.2014.02.003
PMC
PMC4285713
OpenAlex
W2071081262
Cited by
89
References
5
Citations by year
2026: 8 · 2025: 8 · 2024: 7 · 2023: 5 · 2022: 7 · 2021: 8 · 2020: 7 · 2019: 14 · 2018: 3 · 2017: 7 · 2016: 6 · 2015: 6 · 2014: 3
FWCI
1.02
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Institute on Aging (R00AG034988, R01AG045183, R01AG046582)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
Keywords
coherent anti-Stokes Raman scattering microscopy, stimulated Raman scattering microscopy, lipid imaging, chemical imaging, fat metabolism, fat deposition, noninvasive in vivo imaging
MeSH terms
Lipids, Microscopy, Spectrum Analysis, Raman

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