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Cited by 957Spectroscopy Techniques in Biomedical and Chemical ResearchOptical Imaging and Spectroscopy TechniquesSpectroscopy and Quantum Chemical Studies

Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy

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Evans, Conor L., Potma, Eric O., Puoris'haag, Mehron, Côté, Daniel, Lin, Charles P., Xie, X. Sunney·2005·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.0508282102PMC PMC1283840
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Abstract

Imaging living organisms with molecular selectivity typically requires the introduction of specific labels. Many applications in biology and medicine, however, would significantly benefit from a noninvasive imaging technique that circumvents such exogenous probes. In vivo microscopy based on vibrational spectroscopic contrast offers a unique approach for visualizing tissue architecture with molecular specificity. We have developed a sensitive technique for vibrational imaging of tissues by combining coherent anti-Stokes Raman scattering (CARS) with video-rate microscopy. Backscattering of the intense forward-propagating CARS radiation in tissue gives rise to a strong epi-CARS signal that makes in vivo imaging possible. This substantially large signal allows for real-time monitoring of dynamic processes, such as the diffusion of chemical compounds, in tissues. By tuning into the CH 2 stretching vibrational band, we demonstrate CARS imaging and spectroscopy of lipid-rich tissue structures in the skin of a live mouse, including sebaceous glands, corneocytes, and adipocytes, with unprecedented contrast at subcellular resolution.

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Authors
Evans, C. L., Potma, E. O., Puoris'haag, M., Côté, D., Lin, C. P., & Xie, X. S.
Affiliations
  • Harvard University (US)
  • Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138; and Wellman Center for Photomedicine, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2005-11
Volume
102
Issue
46
Pages
16807-16812
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.0508282102
PMC
PMC1283840
OpenAlex
W2148691803
Cited by
957
References
27
Citations by year
2026: 13 · 2025: 29 · 2024: 32 · 2023: 42 · 2022: 38 · 2021: 40 · 2020: 31 · 2019: 45 · 2018: 36 · 2017: 47 · 2016: 70 · 2015: 58 · 2014: 59 · 2013: 54 · 2012: 64
FWCI
21.82
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
  • Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Spectroscopy and Quantum Chemical Studies
Keywords
coherent anti-Stokes Raman scattering microscopy, in vivo tissue imaging, vibrational imaging, noninvasive molecular imaging, skin, sebaceous glands, corneocytes, adipocytes, mouse skin
MeSH terms
Animals, Diffusion, Female, Mice, Inbred BALB C, Mice, Hairless, Microscopy, Mineral Oil, Scattering, Radiation, Skin, Spectrum Analysis, Raman, Mice

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