BLBP-expression in astrocytes during experimental demyelination and in human multiple sclerosis lesions
Abstract
Several lines of evidence indicate that remyelination represents one of the most effective mechanisms to achieve axonal protection. For reasons that are not yet understood, this process is often incomplete or fails in multiple sclerosis (MS). Activated astrocytes appear to be able to boost or inhibit endogenous repair processes. A better understanding of remyelination in MS and possible reasons for its failure is needed. Using the well-established toxic demyelination cuprizone model, we created lesions with either robust or impaired endogenous remyelination capacity. Lesions were analyzed for mRNA expression levels by Affymetrix GeneChip® arrays. One finding was the predominance of immune and stress response factors in the group of genes which were classified as remyelination-supporting factors. We further demonstrate that lesions with impaired remyelination capacity show weak expression of the radial-glia cell marker brain lipid binding protein (BLBP, also called B-FABP or FABP7). The expression of BLBP in activated astrocytes correlates with the presence of oligodendrocyte progenitor cells. BLBP-expressing astrocytes are also detected in experimental autoimmune encephalomyelitis during the remission phase. Furthermore, highest numbers of BLBP-expressing astrocytes were evident in lesions of early MS, whereas significantly less are present at the rim of (chronic)-active lesions from patients with long disease duration. Transfection experiments show that BLBP regulates growth…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Astrocytes show significantly lower gene and protein expression of GFAP after chronic demyelination compared to acute demyelination.
“Since astrocytes show significantly lower gene and protein expression of GFAP after chronic demyelination compared to acute demyelination”
From Glial response during cuprizone-induced de- and remyelination in the CNS: lessons learned (2014) · cites this paper as Kipp et al., 2011b
The activation status of astrocytes appears to be higher after acute demyelination than after chronic demyelination.
“the activation status of astrocytes appears to be higher after acute demyelination (Ludwin, 1980; Kipp et al., 2011b)”
From Glial response during cuprizone-induced de- and remyelination in the CNS: lessons learned (2014) · cites this paper as Kipp et al., 2011b
Under continuing cuprizone treatment, astrogliosis persists and after chronic demyelination the number of astrocytes is similar to acute demyelination.
“Under continuing cuprizone treatment astrogliosis persists and after chronic demyelination the number of astrocytes is similar to acute demyelination (Lindner et al., 2009; Kipp et al., 2011b).”
From Glial response during cuprizone-induced de- and remyelination in the CNS: lessons learned (2014) · cites this paper as Kipp et al., 2011b
Cites
Microglia in the adult brain arise from Ly-6ChiCCR2+ monocytes only under defined host conditions
Mildner, A., Schmidt, H., Nitsche, M., Merkler, D., Hanisch, U.-K., Mack, M., Heikenwalder, M., Brück, W., Priller, J., & Prinz, M. (2007) · Nature Neuroscience · doi:10.1038/nn2015 · Cited by 1,089 · see citing works
TNFα promotes proliferation of oligodendrocyte progenitors and remyelination
Arnett, H. A., Mason, J., Marino, M., Suzuki, K., Matsushima, G. K., & Ting, J. P.-Y. (2001) · Nature Neuroscience · doi:10.1038/nn738 · Cited by 1,012 · see citing works
Myelin Impairs CNS Remyelination by Inhibiting Oligodendrocyte Precursor Cell Differentiation
Kotter, M. R., Li, W.-W., Zhao, C., & Franklin, R. J. M. (2006) · The Journal of Neuroscience · doi:10.1523/jneurosci.2615-05.2006 · Cited by 768 · see citing works
Hyaluronan accumulates in demyelinated lesions and inhibits oligodendrocyte progenitor maturation
Back, S. A., Tuohy, T. M. F., Chen, H., Wallingford, N., Craig, A., Struve, J., Luo, N. L., Banine, F., Liu, Y., Chang, A., et al. (2005) · Nature Medicine · doi:10.1038/nm1279 · Cited by 612 · see citing works
Differentiation of mouse embryonic stem cells into a defined neuronal lineage
Bibel, M., Richter, J., Schrenk, K., Tucker, K. L., Staiger, V., Korte, M., Goetz, M., & Barde, Y.-A. (2004) · Nature Neuroscience · doi:10.1038/nn1301 · Cited by 450 · see citing works
Hashimoto, M., Hossain, M. S., Yamasaki, H., Yazawa, K., & Masumura, S. (1999) · Lipids · doi:10.1007/s11745-999-0481-6 · Cited by 189 · see citing works
Platelet-Derived Growth Factor Promotes Repair of Chronically Demyelinated White Matter
Vana, A. C., Flint, N. C., Harwood, N. E., Le, T. Q., Fruttiger, M., & Armstrong, R. C. (2007) · Journal of Neuropathology & Experimental Neurology · doi:10.1097/nen.0b013e3181587d46 · Cited by 101 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Praet, J., Guglielmetti, C., Berneman, Z., Van der Linden, A., & Ponsaerts, P. (2014) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2014.10.004 · Cited by 435 · see citing works
Glial response during cuprizone-induced de- and remyelination in the CNS: lessons learned
Gudi, V., Gingele, S., Skripuletz, T., & Stangel, M. (2014) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2014.00073 · Cited by 375 · see citing works
“Due to an extensive membrane synthesis oligodendrocytes need to display a high cellular metabolism requiring a large amount of oxygen and adenosintriphosphat (ATP).”
BackgroundInfluentialSelf-citationThe Cuprizone Model: Dos and Do Nots
Zhan, J., Mann, T., Joost, S., Behrangi, N., Frank, M., & Kipp, M. (2020) · Cells · doi:10.3390/cells9040843 · Cited by 171 · see citing works
“Alternatively, it might be that mature oligodendrocytes re-express proteins expressed during oligodendrocyte development, as suggested for astrocytes [38], or that OPCs are activated early during the course of cuprizone-induced demyelination.”
BackgroundSelf-citationCXCL10 Triggers Early Microglial Activation in the Cuprizone Model
Clarner, T., Janssen, K., Nellessen, L., Stangel, M., Skripuletz, T., Krauspe, B., Hess, F.-M., Denecke, B., Beutner, C., Linnartz-Gerlach, B., et al. (2015) · The Journal of Immunology · doi:10.4049/jimmunol.1401459 · Cited by 150 · see citing works
Self-citationNeurodegeneration Triggers Peripheral Immune Cell Recruitment into the Forebrain
Scheld, M., Rüther, B. J., Große-Veldmann, R., Ohl, K., Tenbrock, K., Dreymüller, D., Fallier-Becker, P., Zendedel, A., Beyer, C., Clarner, T., et al. (2016) · The Journal of Neuroscience · doi:10.1523/jneurosci.2456-15.2016 · Cited by 82 · see citing works
Self-citation
- Authors
- Kipp, M., Gingele, S., Pott, F., Clarner, T., van der Valk, P., Denecke, B., Gan, L., Siffrin, V., Zipp, F., Dreher, W., Baumgartner, W., Pfeifenbring, S., Godbout, R., Amor, S., & Beyer, C.
- Affiliations
- RWTH Aachen University (DE)
- Johannes Gutenberg University Mainz (DE)
- University of Bremen (DE)
- Universitätsmedizin Göttingen (DE)
- University of Alberta (CA)
- Queen Mary University of London (GB)
- Corresponding author
- Kipp, M.
- Venue
- Brain, Behavior, and Immunity
- Publisher
- Elsevier BV
- Published
- 2011-11
- Volume
- 25
- Issue
- 8
- Pages
- 1554-1568
- ISSN
- 0889-1591 (ISSN-L)
- OpenAlex
- W1994743569
- Cited by
- 83
- References
- 7
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 2 · 2023: 4 · 2022: 5 · 2021: 5 · 2020: 3 · 2019: 6 · 2018: 2 · 2017: 3 · 2016: 5 · 2015: 10 · 2014: 8 · 2013: 5 · 2012: 11
- FWCI
- 6.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Behavior and Immunity (publishedVersion)
- PubMed (publishedVersion)
- Pure Amsterdam UMC (publishedVersion)
- Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion)
- Digital Academic REpository of VU University Amsterdam (Vrije Universiteit Amsterdam) (submittedVersion)
- MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (submittedVersion)
- GRO (submittedVersion)
- RWTH Publications (RWTH Aachen) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- brain lipid-binding protein, FABP7, astrocytes, remyelination, multiple sclerosis, cuprizone, experimental autoimmune encephalomyelitis, oligodendrocyte precursor cells, demyelination, disease duration, gene expression, growth factor regulation, astrocyte activation
- MeSH terms
- Fatty Acid-Binding Protein 7, Adult, Aged, Animals, Astrocytes, Carrier Proteins, Cell Count, Cuprizone, Demyelinating Diseases, Encephalomyelitis, Autoimmune, Experimental, Female, Glial Fibrillary Acidic Protein, Humans, Immunohistochemistry, Male, Mice, Inbred C57BL, Middle Aged, Multiple Sclerosis, Nerve Tissue Proteins, Platelet-Derived Growth Factor, RNA, Messenger, Transfection, Blotting, Western, Fibroblast Growth Factor 2, Fluorescent Antibody Technique, Indirect, Oligonucleotide Array Sequence Analysis, Tumor Suppressor Proteins, Cell Line, Tumor, Fatty Acid-Binding Proteins, Mice, Osteopontin, Real-Time Polymerase Chain Reaction
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
