Differentiation of mouse embryonic stem cells into a defined neuronal lineage
Abstract
Although it has long been known that cultured embryonic stem cells can generate neurons, the lineage relationships with their immediate precursors remain unclear. We report here that selection of highly proliferative stem cells followed by treatment with retinoic acid generated essentially pure precursors that markers identified as Pax-6-positive radial glial cells. As they do in vivo, these cells went on to generate neurons with remarkably uniform biochemical and electrophysiological characteristics.
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The transgenic cell lines yielded highly pure populations corresponding to ES, NPC, or CN.
“We used transgenic cell lines expressing GFP either from the Oct4, Sox1 (Ying et al., 2003), or Tau (Mapt) (Bibel et al., 2004) loci to obtain highly pure populations corresponding to the respective cell type: ES, NPC, or CN”
From Multiscale 3D Genome Rewiring during Mouse Neural Development (2017) · cites this paper as Bibel et al., 2004
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- Authors
- Bibel, M., Richter, J., Schrenk, K., Tucker, K. L., Staiger, V., Korte, M., Goetz, M., & Barde, Y.-A.
- Affiliations
- Novartis (Switzerland) (CH)
- Heidelberg University (DE)
- Max Planck Institute of Neurobiology (DE)
- University of Basel (CH)
- Corresponding author
- Barde, Yves-Alain
- Venue
- Nature Neuroscience
- Published
- 2004-08-26
- Volume
- 7
- Issue
- 9
- Pages
- 1003-1009
- ISSN
- 1097-6256 (ISSN-L)
- 1546-1726
- OpenAlex
- W1998641354
- Cited by
- 450
- References
- 46
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 6 · 2023: 5 · 2022: 14 · 2021: 6 · 2020: 13 · 2019: 12 · 2018: 17 · 2017: 18 · 2016: 8 · 2015: 22 · 2014: 22 · 2013: 30 · 2012: 46
- FWCI
- 6.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- Max Planck Digital Library (submittedVersion)
- ORCA Online Research @Cardiff (Cardiff University) (submittedVersion)
- Site cant be reached (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- mouse embryonic stem cells, neuronal differentiation, radial glia, Pax6, retinoic acid, neuronal precursors, neuronal lineage, electrophysiological properties
- MeSH terms
- Fatty Acid-Binding Protein 7, PAX6 Transcription Factor, Animals, Bicuculline, Calmodulin-Binding Proteins, Carrier Proteins, Cell Count, Cell Differentiation, Cells, Cultured, Drug Interactions, Electric Stimulation, Embryo, Mammalian, Eye Proteins, Glial Fibrillary Acidic Protein, Immunohistochemistry, Indoles, Intermediate Filament Proteins, Membrane Potentials, Nerve Tissue Proteins, Neuroglia, Neurons, Quinoxalines, Repressor Proteins, Stem Cells, Tetrodotoxin, Time Factors, Tretinoin, Tubulin, Valine, Blotting, Western, Synaptophysin, Receptors, Nerve Growth Factor, Receptors, AMPA, Homeodomain Proteins, Patch-Clamp Techniques, Excitatory Amino Acid Agonists, Excitatory Amino Acid Antagonists, Glutamic Acid, GABA Antagonists, Cell Lineage, Receptor, Nerve Growth Factor, Receptor, trkA, Fatty Acid-Binding Proteins, Mice, Neurogranin, Paired Box Transcription Factors, Nestin
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