Cited by 450Neurogenesis and neuroplasticity mechanismsPluripotent Stem Cells ResearchDevelopmental Biology and Gene Regulation

Differentiation of mouse embryonic stem cells into a defined neuronal lineage

Bibel, Miriam, Richter, Jens, Schrenk, Katrin, Tucker, Kerry Lee, Staiger, Volker, Korte, Martin, Goetz, Magdalena, Barde, Yves-Alain·2004·Nature Neuroscience

Abstract

Although it has long been known that cultured embryonic stem cells can generate neurons, the lineage relationships with their immediate precursors remain unclear. We report here that selection of highly proliferative stem cells followed by treatment with retinoic acid generated essentially pure precursors that markers identified as Pax-6-positive radial glial cells. As they do in vivo, these cells went on to generate neurons with remarkably uniform biochemical and electrophysiological characteristics.

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Crossref · 10.1038/nn1301

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  • The transgenic cell lines yielded highly pure populations corresponding to ES, NPC, or CN.

    “We used transgenic cell lines expressing GFP either from the Oct4, Sox1 (Ying et al., 2003), or Tau (Mapt) (Bibel et al., 2004) loci to obtain highly pure populations corresponding to the respective cell type: ES, NPC, or CN”

    From Multiscale 3D Genome Rewiring during Mouse Neural Development (2017) · cites this paper as Bibel et al., 2004

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Authors
Bibel, M., Richter, J., Schrenk, K., Tucker, K. L., Staiger, V., Korte, M., Goetz, M., & Barde, Y.-A.
Affiliations
  • Novartis (Switzerland) (CH)
  • Heidelberg University (DE)
  • Max Planck Institute of Neurobiology (DE)
  • University of Basel (CH)
Corresponding author
Barde, Yves-Alain
Venue
Nature Neuroscience
Published
2004-08-26
Volume
7
Issue
9
Pages
1003-1009
ISSN
  • 1097-6256 (ISSN-L)
  • 1546-1726
OpenAlex
W1998641354
Cited by
450
References
46
Citations by year
2026: 4 · 2025: 5 · 2024: 6 · 2023: 5 · 2022: 14 · 2021: 6 · 2020: 13 · 2019: 12 · 2018: 17 · 2017: 18 · 2016: 8 · 2015: 22 · 2014: 22 · 2013: 30 · 2012: 46
FWCI
6.29
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • Max Planck Digital Library (submittedVersion)
  • ORCA Online Research @Cardiff (Cardiff University) (submittedVersion)
  • Site cant be reached (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
mouse embryonic stem cells, neuronal differentiation, radial glia, Pax6, retinoic acid, neuronal precursors, neuronal lineage, electrophysiological properties
MeSH terms
Fatty Acid-Binding Protein 7, PAX6 Transcription Factor, Animals, Bicuculline, Calmodulin-Binding Proteins, Carrier Proteins, Cell Count, Cell Differentiation, Cells, Cultured, Drug Interactions, Electric Stimulation, Embryo, Mammalian, Eye Proteins, Glial Fibrillary Acidic Protein, Immunohistochemistry, Indoles, Intermediate Filament Proteins, Membrane Potentials, Nerve Tissue Proteins, Neuroglia, Neurons, Quinoxalines, Repressor Proteins, Stem Cells, Tetrodotoxin, Time Factors, Tretinoin, Tubulin, Valine, Blotting, Western, Synaptophysin, Receptors, Nerve Growth Factor, Receptors, AMPA, Homeodomain Proteins, Patch-Clamp Techniques, Excitatory Amino Acid Agonists, Excitatory Amino Acid Antagonists, Glutamic Acid, GABA Antagonists, Cell Lineage, Receptor, Nerve Growth Factor, Receptor, trkA, Fatty Acid-Binding Proteins, Mice, Neurogranin, Paired Box Transcription Factors, Nestin

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