Cited by 8T-cell and B-cell ImmunologyCell death mechanisms and regulationProtein Tyrosine Phosphatases

BCR-crosslinking induces a transcription of protein phosphatase component G5PR that is required for mature B-cell survival

Huq Ronny, Faisal Mahmudul, Igarashi, Hideya, Sakaguchi, Nobuo·2006·Biochemical and Biophysical Research Communications

Abstract

BCR-crosslinking triggers activation-induced cell death (AICD) selectively in the restricted stage of B-cell differentiation. We examined the transcription of a protein phosphatase subunit G5PR in immature and mature B-cells, because absence of this factor augmented cell sensitivity to AICD, associated with increased activation of JNK and Bim. BCR-crosslinking-induced G5pr transcription in AICD-resistant mature splenic IgM(lo)IgD(hi) B-cells but not in AICD susceptible immature IgM(hi)IgD(lo) B-cells. Thus, G5pr induction correlated with the prevention of AICD; High in mature splenic CD23(hi) B-cells but low in immature B-cells of neonatal mice, sub-lethally irradiated mice, or xid mice. Lack of G5pr upregulation was associated with the prolonged activation of JNK. The G5pr cDNA transfection protected an immature B-cell line WEHI-231 from BCR-mediated AICD. The differential expression of G5PR might be responsible for the antigen-dependent selection of B-cells.

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Crossref · 10.1016/j.bbrc.2005.11.169

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Authors
Huq Ronny, F. M., Igarashi, H., & Sakaguchi, N.
Affiliations
Japan Science and Technology Agency (JP)
Corresponding author
Sakaguchi, Nobuo
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2006-02
Volume
340
Issue
1
Pages
338-346
ISSN
0006-291X (ISSN-L)
OpenAlex
W2094133361
Cited by
8
References
36
Citations by year
2022: 1 · 2018: 2 · 2016: 1 · 2015: 1 · 2012: 1
FWCI
0.12
Type
journal article
Language
en
Open access
closed
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Japan Science and Technology Agency
  • Core Research for Evolutional Science and Technology
  • Ministry of Education, Culture, Sports, Science and Technology
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
Keywords
protein phosphatase 2A, activation-induced cell death, B cell survival, JNK activation, building information modeling, CD23, WEHI-231 cells, xid mice
MeSH terms
Animals, Antigens, Differentiation, B-Lymphocyte, B-Lymphocytes, Cell Differentiation, Cell Line, Cell Survival, Cross-Linking Reagents, Phosphoprotein Phosphatases, Receptors, Antigen, B-Cell, Transcriptional Activation, Cell Proliferation, Mice

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