GABAB receptor subunit 1 binds to proteins affected in 22q11 deletion syndrome
Abstract
GABA(B) receptors mediate slow inhibitory effects of the neurotransmitter gamma-aminobutyric acid (GABA) on synaptic transmission in the central nervous system. They function as heterodimeric G-protein-coupled receptors composed of the seven-transmembrane domain proteins GABA(B1) and GABA(B2), which are linked through a coiled-coil interaction. The ligand-binding subunit GABA(B1) is at first retained in the endoplasmic reticulum and is transported to the cell surface only upon assembly with GABA(B2). Here, we report that GABA(B1), via the coiled-coil domain, can also bind to soluble proteins of unknown function, that are affected in 22q11 deletion/DiGeorge syndrome and are therefore referred to as DiGeorge critical region 6 (DGCR6). In transfected neurons the GABA(B1)-DGCR6 association resulted in a redistribution of both proteins into intracellular clusters. Furthermore, the C-terminus of GABA(B2) interfered with the novel interaction, consistent with heterodimer formation overriding transient DGCR6-binding to GABA(B1). Thus, sequential coiled-coil interactions may direct GABA(B1) into functional receptors.
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DGCR6L has been shown to interact with the gamma-aminobutyric acid GABA-B1 receptor subunit and to be related to anxiety in children with chromosome 22q11.2 deletion syndrome as well as to schizophrenia.
“the DiGeorge critical region 6 and its duplication (DGCR6L) have been shown to interact with the gamma-aminobutyric acid GABA-B1 receptor subunit (Zunner et al., 2010) and to be related to anxiety in children with chromosome 22q11.2 deletion syndrome (Das Chakraborty et al., 2012) as well as to schizophrenia (Liu et al., 2002).”
From Epigenome-wide DNA methylation in unipolar depression: predictive biomarker of antidepressant treatment response? (2024) · cites this paper as Zunner et al., 2010
Cites
Heterodimerization is required for the formation of a functional GABAB receptor
White, J. H. M., Wise, A., Main, M. J., Green, A., Fraser, N. J., Disney, G. H., Barnes, A. A., Emson, P. C., Foord, S. M., & Marshall, F. H. (1998) · Nature · doi:10.1038/25354 · Cited by 1,165 · see citing works
High rates of schizophrenia in adults with velo-cardio-facial syndrome
Murphy, K. C., Jones, L. A., & Owen, M. J. (1999) · Archives of General Psychiatry · doi:10.1001/archpsyc.56.10.940 · Cited by 1,024 · see citing works
Molecular Structure and Physiological Functions of GABA B Receptors
Bettler, B., Kaupmann, K., Mosbacher, J., & Gassmann, M. (2004) · Physiological Reviews · doi:10.1152/physrev.00036.2003 · Cited by 927 · see citing works
Zlokarnik, G., Negulescu, P. A., Knapp, T. E., Mere, L., Burres, N. S., Feng, L., Whitney, M. A., Roemer, K., & Tsien, R. Y. (1998) · Science · doi:10.1126/science.279.5347.84 · Cited by 691 · see citing works
A Trafficking Checkpoint Controls GABAB Receptor Heterodimerization
Margeta, M., Jan, Y. N., & Jan, L. Y. (2000) · Neuron · doi:10.1016/s0896-6273(00)00012-x · Cited by 680 · see citing works
Bowery, N. G., Bettler, B., Froestl, W., Gallagher, J. P., Marshall, F. H., Raiteri, M., Bonner, T. I., & Enna, S. J. (2002) · Pharmacological Reviews · doi:10.1124/pr.54.2.247 · Cited by 601 · see citing works
Role of Heteromer Formation in GABA B Receptor Function
Kuner, R., Köhr, G., Grünewald, S., Eisenhardt, G., Bach, A., & Kornau, H. (1999) · Science · doi:10.1126/science.283.5398.74 · Cited by 563 · see citing works
Self-citationGalarneau, A., Primeau, M., Trudeau, L., et al. (2002) · Nature Biotechnology · doi:10.1038/nbt0602-619 · Cited by 410 · see citing works
Pagano, A., Rovelli, G., Mosbacher, J., Lohmann, T., Duthey, B., Stauffer, D., Ristig, D., Schuler, V., Meigel, I., Lampert, C., et al. (2001) · Journal of Neuroscience · doi:10.1523/jneurosci.21-04-01189.2001 · Cited by 310 · see citing works
Chromosomal microdeletions: dissecting del22q11 syndrome
Lindsay, E. A. (2001) · Nature Reviews Genetics · doi:10.1038/35098574 · Cited by 261 · see citing works
The Ras recruitment system, a novel approach to the study of protein–protein interactions
Broder, Y. C., Katz, S., & Aronheim, A. (1998) · Current Biology · doi:10.1016/s0960-9822(98)70467-1 · Cited by 209 · see citing works
Wehrman, T. S., Kleaveland, B., Her, J. H., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.062043699 · Cited by 194 · see citing works
Calver, A. R., Robbins, M. J., Cosio, C., Rice, S. Q. J., Babbs, A. J., Hirst, W. D., Boyfield, I., Wood, M. D., Russell, R. B., Price, G. W., et al. (2001) · Journal of Neuroscience · doi:10.1523/jneurosci.21-04-01203.2001 · Cited by 164 · see citing works
Locker, J. K., Parton, R. G., Fuller, S. D., Griffiths, G., & Dotti, C. G. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.10.1315 · Cited by 145 · see citing works
The Metabotropic GABAB Receptor Directly Interacts with the Activating Transcription Factor 4
Nehring, R. B., Horikawa, H., Far, O. E., Kneussel, M., Brandstätter, J. H., Stamm, S., Wischmeyer, E., Betz, H., & Karschin, A. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m002727200 · Cited by 126 · see citing works
Association of GABAB Receptors and Members of the 14-3-3 Family of Signaling Proteins
Couve, A., Kittler, J. T., Uren, J. M., Calver, A. R., Pangalos, M. N., Walsh, F. S., & Moss, S. J. (2001) · Molecular and Cellular Neuroscience · doi:10.1006/mcne.2000.0938 · Cited by 124 · see citing works
Heterodimerization of a Functional GABAB Receptor Is Mediated by Parallel Coiled-Coil α-Helices
Kammerer, R. A., Frank, S., Schulthess, T., Landwehr, R., Lustig, A., & Engel, J. (1999) · Biochemistry · doi:10.1021/bi991018t · Cited by 93 · see citing works
Mouse Models of 22q11 Deletion Syndrome
Paylor, R. E., & Lindsay, E. (2006) · Biological Psychiatry · doi:10.1016/j.biopsych.2006.01.018 · Cited by 77 · see citing works
Marlin-1, a Novel RNA-binding Protein Associates with GABA Receptors
Couve, A., Restituito, S., Brandon, J. M., Charles, K. J., Bawagan, H., Freeman, K. B., Pangalos, M. N., Calver, A. R., & Moss, S. J. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m311737200 · Cited by 63 · see citing works
Fritschy, J., Sidler, C. L., Parpan, F., et al. (2004) · The Journal of Comparative Neurology · doi:10.1002/cne.20188 · Cited by 60 · see citing works
Clozapine, GABAB, and the Treatment of Resistant Schizophrenia
Daskalakis, Z. J., & George, T. P. (2009) · Clinical Pharmacology & Therapeutics · doi:10.1038/clpt.2009.115 · Cited by 53 · see citing works
Importance of the γ-Aminobutyric AcidB Receptor C-Termini for G-Protein Coupling
Grünewald, S., Schupp, B. J., Ikeda, S. R., et al. (2002) · Molecular Pharmacology · doi:10.1124/mol.61.5.1070 · Cited by 51 · see citing works
GABAB receptors and synaptic modulation
Kornau, H. (2006) · Cell and Tissue Research · doi:10.1007/s00441-006-0264-7 · Cited by 51 · see citing works
Self-citationHyperdopaminergia and altered locomotor activity in GABAB1‐deficient mice
Vacher, C., Gassmann, M., Desrayaud, S., Challet, É., Bradaïa, A., Hoyer, D., Waldmeier, P. C., Kaupmann, K., Pévet, P., & Bettler, B. (2006) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2006.03806.x · Cited by 48 · see citing works
Demczuk, S. (1996) · Human Molecular Genetics · doi:10.1093/hmg/5.5.633 · Cited by 41 · see citing works
Sauter, K., Grampp, T., Fritschy, J., Kaupmann, K., Bettler, B., Möhler, H., & Benke, D. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m503482200 · Cited by 39 · see citing works
Edelmann, L., Stankiewicz, P., Spiteri, E., Pandita, R., Shaffer, L. G., Lupski, J., & Morrow, B. E. (2001) · Genome Research · doi:10.1101/gr.gr-1431r · Cited by 37 · see citing works
Kantamneni, S., Corrêa, S. A. L., Hodgkinson, G. K., et al. (2006) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2006.04271.x · Cited by 32 · see citing works
Lindsay, E. A., & Baldini, A. (2008) · Cytogenetics and Cell Genetics · doi:10.1159/000134736 · Cited by 15 · see citing works
Biochemical characterisation of the proteins encoded by the DiGeorge critical region 6 (DGCR6) genes
Pfuhl, T., Dürr, M., Spurk, A., et al. (2005) · Human Genetics · doi:10.1007/s00439-005-1267-2 · Cited by 11 · see citing works
Edelmann, L. J., Stankiewicz, P., Spiteri, E. J., et al. (2001) · Genome Research · Cited by 8 · see citing works
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Chakraborty, D., Bernal, A. J., Schoch, K., Howard, T. D., Ip, E. H., Hooper, S. R., Keshavan, M. S., Jirtle, R. L., & Shashi, V (2012) · Translational Psychiatry · doi:10.1038/tp.2012.31 · Cited by 30 · see citing works
Schiele, M. A., Crespo Salvador, O., Lipovsek, J., Schwarte, K., Schlosser, P., Zwanzger, P., Arolt, V., Baune, B. T., Köttgen, A., & Domschke, K. (2024) · International Journal of Neuropsychopharmacology · doi:10.1093/ijnp/pyae045 · Cited by 13 · see citing works
- Authors
- Zunner, D., Deschermeier, C., & Kornau, H.-C.
- Affiliations
- Universität Hamburg (DE)
- Corresponding author
- Kornau, Hans-Christian
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2010-03
- Volume
- 393
- Issue
- 2
- Pages
- 185-189
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W1999664605
- Cited by
- 11
- References
- 31
- Citations by year
- 2024: 2 · 2022: 1 · 2016: 1 · 2015: 1 · 2014: 1 · 2013: 1 · 2012: 3
- FWCI
- 0.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SFB444)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Keywords
- GABAB receptors, 22q11.2 deletion syndrome, coiled-coil domain, protein-protein interaction, receptor heterodimerization
- MeSH terms
- Amino Acid Sequence, Animals, DiGeorge Syndrome, Humans, Molecular Sequence Data, Neurons, Nuclear Proteins, Transfection, Extracellular Matrix Proteins, Protein Structure, Tertiary, Receptors, GABA-B, Two-Hybrid System Techniques, Mice
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