Mitochondrial localization of fission yeast manganese superoxide dismutase is required for its lysine acetylation and for cellular stress resistance and respiratory growth
Abstract
Manganese-dependent superoxide dismutase (MnSOD) is localized in the mitochondria and is important for oxidative stress resistance. Although transcriptional regulation of MnSOD has been relatively well studied, much less is known about the protein's posttranslational regulation. In budding yeast, MnSOD is activated after mitochondrial import by manganese ion incorporation. Here we characterize posttranslational modification of MnSOD in the fission yeast Schizosaccharomyces pombe. Fission yeast MnSOD is acetylated at the 25th lysine residue. This acetylation was diminished by deletion of N-terminal mitochondrial targeting sequence, suggesting that MnSOD is acetylated after import into mitochondria. Mitochondrial localization of MnSOD is not essential for the enzyme activity, but is crucial for oxidative stress resistance and growth under respiratory conditions of fission yeast. These results suggest that, unlike the situation in budding yeast, S. pombe MnSOD is already active even before mitochondrial localization; nonetheless, mitochondrial localization is critical to allow the cell to cope with reactive oxygen species generated inside or outside of mitochondria.
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MnSOD activity and stability are highly regulated by post-translational modifications, including protein acetylation in mitochondria.
“MnSOD activity and stability are highly regulated by post-translational modifications, including protein acetylation in the mitochondria (Takahashi et al., 2011; Tao et al., 2010).”
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- Authors
- Takahashi, H., Suzuki, T., Shirai, A., Matsuyama, A., Dohmae, N., & Yoshida, M.
- Affiliations
- RIKEN (JP)
- Japan Science and Technology Agency (JP)
- Corresponding author
- Takahashi, Hidekazu
- Yoshida, Minoru
- ORCID
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2011-03
- Volume
- 406
- Issue
- 1
- Pages
- 42-46
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W1993090114
- Cited by
- 7
- References
- 19
- Citations by year
- 2026: 1 · 2017: 1 · 2016: 2 · 2012: 3
- FWCI
- 0.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency
- RIKEN Brain Science Institute
- Core Research for Evolutional Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- manganese superoxide dismutase, lysine acetylation, mitochondrial targeting, mitochondrial protein import, post-translational modification, oxidative stress resistance, respiratory growth, Schizosaccharomyces pombe, reactive oxygen species, manganese incorporation
- MeSH terms
- Acetylation, Amino Acid Sequence, Lysine, Mitochondria, Molecular Sequence Data, Oxygen, Protein Processing, Post-Translational, Schizosaccharomyces, Superoxide Dismutase, Oxidative Stress
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