CoChat
Sign Up
Cited by 219CRISPR and Genetic EngineeringCervical Cancer and HPV ResearchVirus-based gene therapy research

In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by CRISPR/Cas9

Zhen, Shuai, Hua, Ling, Takahashi, Y., Narita, S., Liu, Yun-Hui, Li, Yan·2014·Biochemical and Biophysical Research Communications
DOI 10.1016/j.bbrc.2014.07.014
Open this paper in CoChat — free account

Abstract

Deregulated expression of high-risk human papillomavirus oncogenes (E6 and E7) is a pivotal event for pathogenesis and progression in cervical cancer. Both viral oncogenes are therefore regarded as ideal therapeutic targets. In the hope of developing a gene-specific therapy for HPV-related cancer, we established CRISPR/Cas9 targeting promoter of HPV 16 E6/E7 and targeting E6, E7 transcript, transduced the CRISPR/Cas9 into cervical HPV-16-positive cell line SiHa. The results showed that CRISPR/Cas9 targeting promoter, as well as targeting E6 and E7 resulted in accumulation of p53 and p21 protein, and consequently remarkably reduced the abilities of proliferation of cervical cancer cells in vitro. Then we inoculated subcutaneously cells into nude mice to establish the transplanted tumor animal models, and found dramatically inhibited tumorigenesis and growth of mice incubated by cells with CRISPR/Cas9 targeting (promoter+E6+E7)-transcript. Our results may provide evidence for application of CRISPR/Cas9 targeting HR-HPV key oncogenes, as a new treatment strategy, in cervical and other HPV-associated cancer therapy.

Integrity signals

Registry

Crossref · 10.1016/j.bbrc.2014.07.014

Cites

38 of 38 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Zhen et al reported that transfection of Cas9 and sgRNAs targeting HPV16 E6 or E7 into HPV-16-positive cervical carcinoma cell lines SiHa resulted in inhibition of cancer cell proliferation in vitro and in a xenograft mouse model.

    “Zhen et al 41 reported that the transfection of Cas9 and sgRNAs targeting HPV16 E6 or E7 into HPV-16-positive cervical carcinoma cell lines SiHa resulted in the inhibition of cancer cell proliferation both in vitro and in xenograft mouse model.”

    From CRISPR-Cas9: a new and promising player in gene therapy (2015) · cites this paper as [41]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Zhen, S., Hua, L., Takahashi, Y., Narita, S., Liu, Y.-H., & Li, Y.
Affiliations
Kyoto University (JP)
Corresponding author
Zhen, Shuai
ORCID
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2014-08
Volume
450
Issue
4
Pages
1422-1426
ISSN
0006-291X (ISSN-L)
DOI
10.1016/j.bbrc.2014.07.014
OpenAlex
W1979954728
Semantic Scholar
c18ef9fe844f241f8c0ec4c5f9db885600450a69
Cited by
219
References
38
Citations by year
2026: 7 · 2025: 8 · 2024: 19 · 2023: 13 · 2022: 19 · 2021: 24 · 2020: 21 · 2019: 17 · 2018: 30 · 2017: 21 · 2016: 22 · 2015: 16 · 2014: 2
FWCI
8.33
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
Massachusetts Institute of Technology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Health Sciences › Medicine › Epidemiology › Cervical Cancer and HPV Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Keywords
CRISPR/Cas9, HPV16, cervical cancer, HPV E6 oncogene, HPV E7 oncogene, gene-specific therapy, promoter targeting, p53, p21, cell proliferation inhibition, carcinogenesis, SiHa cells, HPV-associated cancers
MeSH terms
Base Sequence, Cell Division, Uterine Cervical Neoplasms, Female, Humans, DNA Primers, Cell Line, Tumor, Alphapapillomavirus, Clustered Regularly Interspaced Short Palindromic Repeats, In Vitro Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review