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Virion-incorporated alpha-enolase suppresses the early stage of HIV-1 reverse transcription
Abstract
Human immunodeficiency virus type-1 (HIV-1) particles contain not only viral-encoded but also host-encoded proteins. Interestingly, several studies showed that host proteins play a critical role in viral infectivity, replication and/or immunoreactivity in the next target cells. Here, we show that alpha-enolase (ENO1) is incorporated into HIV-1 virions and the virion-incorporated ENO1 prevents the early stage of HIV-1 reverse transcription. We found that viral particles contain two isoforms of ENO1 with different isoelectric points by two-dimensional electrophoresis. Suppression of ENO1 expression by RNA interference in the HIV-1 producer cells decreased ENO1 incorporation into virions without altering the packaging of viral structural proteins and viral production but increased viral infectivity. Although the low-level-ENO1-packaging virus maintained comparable levels of reverse transcriptase activity, viral genomic RNA and tRNA Lys3 packaging to the control virus, its levels of early cDNA products of reverse transcription were higher than those of the control virus. In contrast, the high-level-ENO1-packaging virus, which was produced from ENO1-overexpressing cells, showed decreased infectivity and the levels of early cDNA products. Taken together, these findings reveal a novel function of ENO1 as a negative regulation factor targeting HIV-1 reverse transcription.
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MT1-MMP has been shown to shed LRP1 in breast carcinoma MCF7 cells, vascular smooth muscle cells, and cartilage.
“Recently, MT1-MMP has been shown to shed LRP1 in breast carcinoma MCF7 cells as well as in vascular smooth muscle cells and in cartilage21,45,46.”
From Membrane type 1 matrix metalloproteinase promotes LDL receptor shedding and accelerates the development of atherosclerosis (2021) · cites this paper as [46]
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Cites
Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts
Mangeat, B., Turelli, P., Caron, G., Friedli, M., Perrin, L., & Trono, D. (2003) · Nature · doi:10.1038/nature01709 · Cited by 1,486
Chertova, E., Chertov, O., Coren, L. V., Roser, J. D., Trubey, C. M., Bess, J. W., Sowder, R. C., Barsov, E., Hood, B. L., Fisher, R. J., et al. (2006) · Journal of Virology · doi:10.1128/jvi.01013-06 · Cited by 462
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Research works printed but not linked: 21
- Ott, 1997, Cellular proteins in HIV virions, Rev. Med. Virol., 7, 167, 10.1002/(SICI)1099-1654(199709)7:3<167::AID-RMV199>3.0.CO;2-K · DOI
- Sheehy, 2002, Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein, Nature, 418, 646, 10.1038/nature00939 · DOI
- Kishimoto, 2012, Glyceraldehyde 3-phosphate dehydrogenase negatively regulates human immunodeficiency virus type 1 infection, Retrovirology, 9, 107, 10.1186/1742-4690-9-107 · DOI
- Kim, 2005, Multifaceted roles of glycolytic enzymes, Trends Biochem. Sci., 30, 142, 10.1016/j.tibs.2005.01.005 · DOI
- Diaz-Ramos, 2012, Alpha-enolase, a multifunctional protein: its role on pathophysiological situations, J. Biomed. Biotechnol., 156795
- Ogino, 2001, Enolase, a cellular glycolytic enzyme, is required for efficient transcription of Sendai virus genome, Biochem. Biophys. Res. Co., 285, 447, 10.1006/bbrc.2001.5160 · DOI
- Munoz, 2013, Proteomic identification of dengue virus binding proteins in Aedes aegypti mosquitoes and Aedes albopictus cells, Biomed. Res. Int., 875959
- Lee, 2009, A strongly transdominant mutation in the human immunodeficiency virus type 1 gag gene defines an achilles heel in the virus life cycle, J. Virol., 83, 8536, 10.1128/JVI.00317-09 · DOI · Paper page
- Misumi, 2010, Uncoating of human immunodeficiency virus type 1 requires prolyl isomerase Pin1, J. Biol. Chem., 285, 25185, 10.1074/jbc.M110.114256 · DOI
- Marechal, 1998, Cytosolic Gag p24 as an index of productive entry of human immunodeficiency virus type 1, J. Virol., 72, 2208, 10.1128/JVI.72.3.2208-2212.1998 · DOI
- Zhou, 2010, Mass spectrometry analysis of the post-translational modifications of alpha-enolase from pancreatic ductal adenocarcinoma cells, J. Proteome Res., 9, 2929, 10.1021/pr901109w · DOI
- Trubey, 2003, Quantitation of HLA class II protein incorporated into human immunodeficiency type 1 virions purified by anti-CD45 immunoaffinity depletion of microvesicles, J. Virol., 77, 12699, 10.1128/JVI.77.23.12699-12709.2003 · DOI
- Kishimoto, 2016, The C-terminal domain of glyceraldehyde 3-phosphate dehydrogenase plays an important role in suppression of tRNALys3 packaging into human immunodeficiency virus type-1 particles, Biochem. Biophys. Rep., 8, 325
- Ott, 1996, Cytoskeletal proteins inside human immunodeficiency virus type 1 virions, J. Virol., 70, 7734, 10.1128/JVI.70.11.7734-7743.1996 · DOI
- Rahman, 2014, Investigating the role of F-Actin in human immunodeficiency virus assembly by live-cell microscopy, J. Virol., 88, 7904, 10.1128/JVI.00431-14 · DOI
- Gabor, 2002, Effect of altering the tRNA(3)(Lys) concentration in human immunodeficiency virus type 1 upon its annealing to viral RNA, GagPol incorporation, and viral infectivity, J. Virol., 76, 9096, 10.1128/JVI.76.18.9096-9102.2002 · DOI · Paper page
- Saadatmand, 2012, Aspects of HIV-1 assembly that promote primer tRNA(Lys3) annealing to viral RNA, Virus Res., 169, 340, 10.1016/j.virusres.2012.06.001 · DOI · Paper page
- Stark, 1998, Human immunodeficiency virus type 1 (HIV-1) viral protein R (Vpr) interacts with Lys-tRNA synthetase: implications for priming of HIV-1 reverse transcription, J. Virol., 72, 3037, 10.1128/JVI.72.4.3037-3044.1998 · DOI
- Sleiman, 2012, Initiation of HIV-1 reverse transcription and functional role of nucleocapsid-mediated tRNA/viral genome interactions, Virus Res., 169, 324, 10.1016/j.virusres.2012.06.006 · DOI
- Barrero, 2013, HIV-1 Vpr modulates macrophage metabolic pathways: a SILAC-based quantitative analysis, Plos One, 8, e68376, 10.1371/journal.pone.0068376 · DOI
- Sen, 2016, Non-metabolic role of PKM2 in regulation of the HIV-1 LTR, J. Cell Physiol., 232, 517, 10.1002/jcp.25445 · DOI
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Alabi, A., Xia, X.-D., Gu, H.-M., Wang, F., Deng, S.-J., Yang, N., Adijiang, A., Douglas, D. N., Kneteman, N. M., Xue, Y., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-22167-3 · Cited by 63
Metadata
- Authors
- Kishimoto, N., Iga, N., Yamamoto, K., Takamune, N., & Misumi, S.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2017-03
- Volume
- 484
- Issue
- 2
- Pages
- 278-284
- ISSN
- 0006-291X
- Cited by
- 20
- References
- 2
- Language
- en
- Funders
- KUMAYAKU Alumni Research Foundation
- JSPS KAKENHI (15H04659, 16K18922)
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