Crystal structure of SPSB2 in complex with a rational designed RGD-containing cyclic peptide inhibitor of SPSB2-iNOS interaction

<i>Coot</i> : model-building tools for molecular graphics

Emsley, P., & Cowtan, K. (2004) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444904019158 · Cited by 30,571 · see citing works

Overview of the <i>CCP</i> 4 suite and current developments

Winn, M. D., Ballard, C. C., Cowtan, K. D., Dodson, E. J., Emsley, P., Evans, P. R., Keegan, R. M., Krissinel, E. B., Leslie, A. G. W., McCoy, A., et al. (2011) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444910045749 · Cited by 12,353 · see citing works

REFMAC 5 for the refinement of macromolecular crystal structures

Murshudov, G. N., Skubák, P., Lebedev, A. A., Pannu, N. S., Steiner, R. A., Nicholls, R. A., Winn, M., Long, F., & Vagin, A. A. (2011) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444911001314 · Cited by 8,385 · see citing works

Nitric oxide synthases: structure, function and inhibition

ALDERTON, W. K., COOPER, C. E., & KNOWLES, R. G. (2001) · Biochemical Journal · doi:10.1042/bj3570593 · Cited by 4,110 · see citing works

Nitric oxide synthases: structure, function and inhibition

ALDERTON, W. K., Cooper, C. E., & Knowles, R. G. (2001) · Biochemical Journal · doi:10.1042/0264-6021:3570593 · Cited by 4,106 · see citing works

Integrins in cancer: biological implications and therapeutic opportunities

Desgrosellier, J. S., & Cheresh, D. A. (2009) · Nature reviews. Cancer · doi:10.1038/nrc2748 · Cited by 3,721 · see citing works

Molecular replacement with MOLREP

Vagin, A. A., & Teplyakov, A. (2009) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909042589 · Cited by 3,580 · see citing works

iMOSFLM: a new graphical interface for diffraction-image processing withMOSFLM

Battye, T. G. G., Kontogiannis, L., Johnson, O., Powell, H. R., & Leslie, A. G. W. (2011) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444910048675 · Cited by 2,869 · see citing works

Crystal Structure of the Extracellular Segment of Integrin αVβ3 in Complex with an Arg-Gly-Asp Ligand

Xiong, J.-P., Stehle, T., Zhang, R., Joachimiak, A., Frech, M., Goodman, S. L., & Arnaout, M. A. (2002) · Science · doi:10.1126/science.1069040 · Cited by 1,617 · see citing works

An introduction to data reduction: space-group determination, scaling and intensity statistics

Evans, P. R. (2011) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s090744491003982x · Cited by 1,507 · see citing works

RGD-Based Strategies To Target Alpha(v) Beta(3) Integrin in Cancer Therapy and Diagnosis

Danhier, F., Breton, A. L., & Véronique Préat, A. (2012) · Molecular Pharmaceutics · doi:10.1021/mp3002733 · Cited by 959 · see citing works

Inducible nitric oxide synthase (iNOS) in tumor biology: the two sides of the same coin

Lechner, M., Lirk, P., & Rieder, J. (2005) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2005.04.004 · Cited by 410 · see citing works

The Functions and Applications of RGD in Tumor Therapy and Tissue Engineering

Wang, F., Li, Y., Shen, Y., Wang, A., Wang, S., & Xie, T. (2013) · International Journal of Molecular Sciences · doi:10.3390/ijms140713447 · Cited by 261 · see citing works

Clustering and Internalization of Integrin αvβ3 With a Tetrameric RGD-synthetic Peptide.

Lucie, S., Elisabeth, G., Stéphanie, F., Guy, S., Amandine, H., Corinne, A.-R., Didier, B., Catherine, S., Alexeï, G., Pascal, D., et al. (2009) · Molecular Therapy · doi:10.1038/mt.2009.29 · Cited by 137 · see citing works

Applications of saturation transfer difference NMR in biological systems

Bhunia, A., Bhattacharjya, S., & Chatterjee, S. (2011) · Drug Discovery Today · doi:10.1016/j.drudis.2011.12.016 · Cited by 137 · see citing works

Structural basis for pure antagonism of integrin αVβ3 by a high-affinity form of fibronectin

Agthoven, J. F. V., Xiong, J.-P., Alonso, J. L., Rui, X., Adair, B. D., Goodman, S. L., & Arnaout, M. A. (2014) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2797 · Cited by 135 · see citing works

Structural Basis for Protein Recognition by B30.2/SPRY Domains

Woo, J., Suh, H.-Y., Park, S., & Oh, B. (2006) · Molecular Cell · doi:10.1016/j.molcel.2006.11.009 · Cited by 126 · see citing works

Ubiquitination of inducible nitric oxide synthase is required for its degradation

Kolodziejski, P. J., Musial, A., Koo, J.-S., & Eissa, N. T. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.192345199 · Cited by 119 · see citing works

Cancer cell metabolism and the modulating effects of nitric oxide

Chang, C.-F., Diers, A. R., & Hogg, N. (2014) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2014.11.012 · Cited by 101 · see citing works

The SPRY domain–containing SOCS box protein SPSB2 targets iNOS for proteasomal degradation

Kuang, Z., Lewis, R. S., Curtis, J., Zhan, Y., Saunders, B. M., Babon, J. J., Kolesnik, T. B., Low, A., Masters, S. L., Willson, T. A., et al. (2010) · The Journal of Cell Biology · doi:10.1083/jcb.200912087 · Cited by 101 · see citing works

Integrin αvβ3-Mediated Activation of Apoptosis

Brassard, D. L., Maxwell, E., Malkowski, M. G., Nagabhushan, T. L., Kumar, C. C., & Armstrong, L. (1999) · Experimental Cell Research · doi:10.1006/excr.1999.4559 · Cited by 100 · see citing works

Tumors face NO problems?

Lancaster, J. R., & Xie, K. (2006) · Cancer Research · doi:10.1158/0008-5472.can-05-2900 · Cited by 98 · see citing works

The αvβ3 Integrin Regulates α5β1-mediated Cell Migration toward Fibronectin

Simon, K. O., Nutt, E. M., Abraham, D. G., Rodan, G. A., & Duong, L. T. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.46.29380 · Cited by 91 · see citing works

The SPRY domain of SSB-2 adopts a novel fold that presents conserved Par-4–binding residues

Masters, S. L., Yao, S., Willson, T. A., Zhang, J., Palmer, K. R., Smith, B. J., Babon, J. J., Nicola, N. A., Norton, R. S., & Nicholson, S. E. (2005) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1034 · Cited by 82 · see citing works

Regulation of Inducible Nitric-oxide Synthase by the SPRY Domain- and SOCS Box-containing Proteins

Nishiya, T., Matsumoto, K., Maekawa, S., Kajita, E., Horinouchi, T., Fujimuro, M., Ogasawara, K., Uehara, T., & Miwa, S. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.190678 · Cited by 72 · see citing works

Structural Basis for Par-4 Recognition by the SPRY Domain- and SOCS Box-Containing Proteins SPSB1, SPSB2, and SPSB4

Filippakopoulos, P., Low, A., Sharpe, T., Uppenberg, J., Yao, S., Kuang, Z., Savitsky, P. A., Lewis, R. S., Nicholson, S. E., Norton, R. S., et al. (2010) · Journal of Molecular Biology · doi:10.1016/j.jmb.2010.06.017 · Cited by 67 · see citing works

Nitric oxide synthase gene therapy enhances the toxicity of cisplatin in cancer cells

Adams, C., McCarthy, H. O., Coulter, J. A., Worthington, J., Murphy, C., Robson, T., & Hirst, D. G. (2008) · The Journal of Gene Medicine · doi:10.1002/jgm.1280 · Cited by 64 · see citing works

TLR Regulation of SPSB1 Controls Inducible Nitric Oxide Synthase Induction

Lewis, R. S., Kolesnik, T. B., Kuang, Z., D’Cruz, A. A., Blewitt, M. E., Masters, S. L., Low, A., Willson, T. A., Norton, R. S., & Nicholson, S. E. (2011) · The Journal of Immunology · doi:10.4049/jimmunol.1002993 · Cited by 57 · see citing works

Protein–protein interactions involving inducible nitric oxide synthase

Zhang, W., Kuncewicz, T., Yu, Z. ‐., Zou, L., Xu, X., & Kone, B. C. (2003) · Acta Physiologica Scandinavica · doi:10.1046/j.1365-201x.2003.01119.x · Cited by 49 · see citing works

SPRY Domain-Containing SOCS Box Protein 2: Crystal Structure and Residues Critical for Protein Binding

Kuang, Z., Yao, S., Xu, Y., Lewis, R. S., Low, A., Masters, S. L., Willson, T. A., Kolesnik, T. B., Nicholson, S. E., Garrett, T., et al. (2009) · Journal of Molecular Biology · doi:10.1016/j.jmb.2008.12.078 · Cited by 44 · see citing works

Site-directed delivery of nitric oxide to cancers

Sharma, K., & Chakrapani, H. (2014) · Nitric Oxide · doi:10.1016/j.niox.2014.07.005 · Cited by 43 · see citing works

A Potent Cyclic Peptide Targeting SPSB2 Protein as a Potential Anti-infective Agent

Yap, B. K., Leung, E. W. W., Yagi, H., Galea, C. A., Chhabra, S., Chalmers, D. K., Nicholson, S. E., Thompson, P. E., & Norton, R. S. (2014) · Journal of Medicinal Chemistry · doi:10.1021/jm500596j · Cited by 35 · see citing works

19F NMR as a Probe of Ligand Interactions with the iNOS Binding site of SPRY Domain‐Containing SOCS Box Protein 2

Leung, E. W. W., Yagi, H., Harjani, J. R., Mulcair, M. D., Scanlon, M. J., Baell, J. B., & Norton, R. S. (2014) · Chemical Biology & Drug Design · doi:10.1111/cbdd.12355 · Cited by 27 · see citing works

Design, Synthesis, and Characterization of Cyclic Peptidomimetics of the Inducible Nitric Oxide Synthase Binding Epitope That Disrupt the Protein–Protein Interaction Involving SPRY Domain-Containing Suppressor of Cytokine Signaling Box Protein (SPSB) 2 and Inducible Nitric Oxide Synthase

Harjani, J. R., Yap, B. K., Leung, E. W. W., Lucke, A. J., Nicholson, S. E., Scanlon, M. J., Chalmers, D. K., Thompson, P. E., Norton, R. S., & Baell, J. B. (2016) · Journal of Medicinal Chemistry · doi:10.1021/acs.jmedchem.6b00386 · Cited by 25 · see citing works

The ECS(SPSB) E3 ubiquitin ligase is the master regulator of the lifetime of inducible nitric-oxide synthase

Matsumoto, K., Nishiya, T., Maekawa, S., Horinouchi, T., Ogasawara, K., Uehara, T., & Miwa, S. (2011) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2011.04.103 · Cited by 22 · see citing works

Redox‐stable cyclic peptide inhibitors of the SPSB2–iNOS interaction

Yap, B. K., Harjani, J. R., Leung, E. W. W., Nicholson, S. E., Scanlon, M. J., Chalmers, D. K., Thompson, P. E., Baell, J. B., & Norton, R. S. (2016) · FEBS Letters · doi:10.1002/1873-3468.12115 · Cited by 19 · see citing works

Letter to the Editor: Backbone 1H, 13C and 15N assignments of the 25 kDa SPRY domain-containing SOCS box protein 2 (SSB-2)

Yao, S., Masters, S. L., Zhang, J., Palmer, K. R., Babon, J. J., Nicola, N. A., Nicholson, S. E., & Norton, R. S. (2005) · Journal of Biomolecular NMR · doi:10.1007/s10858-004-6924-6 · Cited by 14 · see citing works

Targeted protein degradation: expanding the toolbox

Schapira, M., Calabrese, M. F., Bullock, A. N., & Crews, C. M. (2019) · Nature Reviews Drug Discovery · doi:10.1038/s41573-019-0047-y · Cited by 960 · see citing works

E3 Ligase Ligands for PROTACs: How They Were Found and How to Discover New Ones

Ishida, T., & Ciulli, A. (2020) · SLAS DISCOVERY · doi:10.1177/2472555220965528 · Cited by 395 · see citing works

Crystal Structures of Protein-Bound Cyclic Peptides

Malde, A. K., Hill, T. A., Iyer, A., & Fairlie, D. P. (2019) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00807 · Cited by 113 · see citing works

Docking Flexible Cyclic Peptides with AutoDock CrankPep

Zhang, Y., & Sanner, M. F. (2019) · Journal of Chemical Theory and Computation · doi:10.1021/acs.jctc.9b00557 · Cited by 61 · see citing works

In silico, in vitro antioxidant and density functional theory based structure activity relationship studies of plant polyphenolics as prominent natural antioxidants

Rammohan, A., Bhaskar, B. V., Camilo, A., Gunasekar, D., Gu, W., & Zyryanov, G. V. (2020) · Arabian Journal of Chemistry · doi:10.1016/j.arabjc.2019.12.017 · Cited by 37 · see citing works

A Cyclic Peptide Inhibitor of the iNOS–SPSB Protein–Protein Interaction as a Potential Anti-Infective Agent

Sadek, M. M., Barlow, Ν., Leung, E. W. W., Williams‐Noonan, B. J., Yap, B. K., Shariff, F. M., Caradoc-Davies, T. T., Nicholson, S. E., Chalmers, D. K., Thompson, P. E., et al. (2018) · ACS Chemical Biology · doi:10.1021/acschembio.8b00561 · Cited by 21 · see citing works

A strategy to assess the cellular activity of E3 ligase components against neo-substrates using electrophilic probes

Pinch, B. J., Buckley, D. L., Gleim, S., Brittain, S. M., Tandeske, L., D'Alessandro, P. L., Hauseman, Z. J., Lipps, J., Xu, L., Harvey, E. P., et al. (2022) · Cell Chemical Biology · doi:10.1016/j.chembiol.2021.08.007 · Cited by 20 · see citing works

Selection of aptamers against Lactoferrin based on silver enhanced and fluorescence-activated cell sorting

Fang, Y., Li, H., Sun, W., Zhao, Y., Xu, D., & He, F. (2018) · Talanta · doi:10.1016/j.talanta.2018.09.063 · Cited by 20 · see citing works

Structural basis for the regulation of inducible nitric oxide synthase by the SPRY domain-containing SOCS box protein SPSB2, an E3 ubiquitin ligase

Li, K., You, T., Zhao, P., Luo, Y., Zhang, D., Wei, H. J., Wang, Y., Yang, J., Guan, X., & Kuang, Z. (2021) · Nitric Oxide · doi:10.1016/j.niox.2021.04.004 · Cited by 11 · see citing works

SPRY domain-containing SOCS box protein 2 complexed with WDINNN(BAL) cyclic peptide inhibitor

Law, R. H. P., Caradoc-Davies, T. T., & Norton, R. S. (2018) · Worldwide Protein Data Bank · doi:10.2210/pdb6dn5/pdb · Cited by 9 · see citing works

Crystal structure of the SPRY domain-containing protein 7 reveals unique structural features

Yang, J., Guan, X., Zhang, D., Zhao, P., Guo, S., & Kuang, Z. (2020) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2020.07.076 · Cited by 8 · see citing works

A reliable, quick and universally applicable method for monitoring aptamer SELEX progress

Baba, S. A., Jain, S., & Navani, N. K. (2021) · Gene · doi:10.1016/j.gene.2021.145416 · Cited by 7 · see citing works

Backbone 1H, 13C, and 15N resonance assignments of the PRY-SPRY domain of RNF135

Zhang, D., Wei, H. J., Xue, H., Guo, S., Wu, B., & Kuang, Z. (2019) · Biomolecular NMR Assignments · doi:10.1007/s12104-019-09895-w · Cited by 6 · see citing works

Crystal structure of the SPRY domain of human SPSB2 in the apo state

Luo, Y., Li, K., Yang, J., Zhang, D., Zhou, Y., & Kuang, Z. (2019) · Acta Crystallographica Section F Structural Biology Communications · doi:10.1107/s2053230x1900623x · Cited by 3 · see citing works

HFGuidedDesign: de novo design of cyclic peptide binders via structure-guided discrete diffusion

Haomeng, H., Zhu, R., Ning, Z., Zhang, C., Shang, T., Li, C., Guo, J., Wang, X., & Duan, H. (2026) · Chemical Science · doi:10.1039/d6sc02631a · Cited by 2 · see citing works

Subtle Structural Differences Affect the Inhibitory Potency of RGD-Containing Cyclic Peptide Inhibitors Targeting SPSB Proteins

Li, K., Luo, Y., Hu, W., Yang, J., Zhang, D., Wei, H. J., You, T., Lin, H., & Kuang, Z. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25126764 · Cited by 2 · see citing works

The B30.2/SPRY-Domain: A Versatile Binding Scaffold in Supramolecular Assemblies of Eukaryotes

Mittl, P. R. E., & Beer, H. (2025) · Crystals · doi:10.3390/cryst15030281 · Cited by 1 · see citing works

Overcoming Ligand Discovery Challenges: Developing Peptide-Based Tracers for SPSB2

Lenz, C., Elson, L., Dopfer, J., Farges, F., Krämer, A., Löhr, F., Müller, S., Guéret, S. M., Waldmann, H., Dötsch, V., et al. (2025) · ACS Chemical Biology · doi:10.1021/acschembio.5c00702

Coopération entre les isoformes TAp73 et la signalisation TGF-β dans la régulation de l'expression de la NO Synthase inductible

Cabrié, A. (2017) · doi:10.70675/df567380z9a01z4262z9a43z7666c8df7b0f

Structure-based design of peptide inhibitors targeting the SPSB2–iNOS interaction: a computational approach to modulating nitric oxide signaling

Shah, S., Mehmood, A., Wenjia, Z., Ahmad, M., Zhao, Y., & Zhou, J. (2026) · Journal of Biomolecular Structure and Dynamics · doi:10.1080/07391102.2026.2710745

Subtle Structural Differences Affect the Inhibitory Potency of RGD-Containing Cyclic Peptide Inhibitors Targeting SPSB Proteins

Li, K., Luo, Y., Hu, W., Yang, J., Zhang, D., Wei, H. J., You, T., Lin, H., & Kuang, Z. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25126764 · Cited by 2 · see citing works

A Cyclic Peptide Inhibitor of the iNOS–SPSB Protein–Protein Interaction as a Potential Anti-Infective Agent

Sadek, M. M., Barlow, Ν., Leung, E. W. W., Williams‐Noonan, B. J., Yap, B. K., Shariff, F. M., Caradoc-Davies, T. T., Nicholson, S. E., Chalmers, D. K., Thompson, P. E., et al. (2018) · ACS Chemical Biology · doi:10.1021/acschembio.8b00561 · Cited by 21 · see citing works

Inhibitors of SPSB-iNOS interactions as a potential novel class of anti-infectives

Yap, B. K. (2017) · Figshare · doi:10.4225/03/58b8ad3057b5b · Cited by 1 · see citing works

Molecular Insights into the Interaction Between the SPRY Domain-Containing SOCS Box Protein SPSB2 and Peptides Based on the Binding Motif from iNOS

Leung, E. W. W., Mulcair, M. D., Yap, B. K., Nicholson, S. E., Scanlon, M. J., & Norton, R. S. (2016) · Australian Journal of Chemistry · doi:10.1071/ch16510 · Cited by 11 · see citing works

Crystal structure of the SPRY domain of human SPSB2 in the apo state

Luo, Y., Li, K., Yang, J., Zhang, D., Zhou, Y., & Kuang, Z. (2019) · Acta Crystallographica Section F Structural Biology Communications · doi:10.1107/s2053230x1900623x · Cited by 3 · see citing works