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Long-term storage of lipid-like nanoparticles for mRNA delivery
Open accessAbstract
Lipid-like nanoparticles (LLNs) have been extensively explored for messenger RNA (mRNA) delivery in various biomedical applications. However, the long-term storage of these nanoparticles is still a challenge for their clinical translation. In this study, we investigated a series of conditions for the long-term storage of LLNs with encapsulation of mRNA. We evaluated the stability of LLNs with different concentrations of cryoprotectants (sucrose, trehalose or mannitol) under the conditions of freezing or lyophilization processes. Through in vitro and in vivo mRNA delivery studies, we identified the optimal storage condition, and found that the addition with 5% (w/v) sucrose or trehalose to LLNs could remain their mRNA delivery efficiency for at least three months in the liquid nitrogen storage condition.
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“For example, the addition of 5% (w/v) sucrose or trehalose to lipid nanoparticle–mRNA formulations, stored in liquid nitrogen, allows maintenance of mRNA delivery efficacy for at least 3 months in vivo168.”
From Lipid nanoparticles for mRNA delivery (2021) · cites this paper as [168]
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“Storage conditions of lipid nanoparticle–mRNA formulations are an important consideration for their clinical translation, because storage (aqueous, freezing and lyophilized storage) and the type of cryoprotectants (sucrose, trehalose or mannitol) affect the long-term stability of lipid nanoparticle–mRNA formulations168.”
From Lipid nanoparticles for mRNA delivery (2021) · cites this paper as [168]
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Multivalent ionizable lipid-polypeptides for tumor-confined mRNA transfection
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Metadata
- Authors
- Zhao, P., Hou, X., Yan, J., Du, S., Xue, Y., Li, W., Xiang, G., & Dong, Y.
- Affiliations
- Huazhong University of Science and Technology (CN)
- The Ohio State University (US)
- Corresponding author
- Xiang, Guangya
- Dong, Yizhou
- Venue
- Bioactive Materials
- Publisher
- Elsevier BV
- Published
- 2020-06
- Volume
- 5
- Issue
- 2
- Pages
- 358-363
- ISSN
- 2097-1192 (ISSN-L)
- 2452-199X
- PMC
- PMC7078456
- OpenAlex
- W3011313923
- Cited by
- 233
- References
- 34
- Citations by year
- 2026: 17 · 2025: 39 · 2024: 48 · 2023: 53 · 2022: 47 · 2021: 24 · 2020: 4
- FWCI
- 10.38
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Bioactive Materials (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ohio State University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Keywords
- mRNA delivery, long-term storage, cryoprotectants, sucrose, trehalose, mannitol, freeze-drying, freezing, liquid nitrogen storage, nanoparticle stability
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