Cited by 233RNA Interference and Gene DeliveryNanoparticle-Based Drug DeliveryAdvancements in Transdermal Drug Delivery

The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.

Long-term storage of lipid-like nanoparticles for mRNA delivery

Open access

Abstract

Lipid-like nanoparticles (LLNs) have been extensively explored for messenger RNA (mRNA) delivery in various biomedical applications. However, the long-term storage of these nanoparticles is still a challenge for their clinical translation. In this study, we investigated a series of conditions for the long-term storage of LLNs with encapsulation of mRNA. We evaluated the stability of LLNs with different concentrations of cryoprotectants (sucrose, trehalose or mannitol) under the conditions of freezing or lyophilization processes. Through in vitro and in vivo mRNA delivery studies, we identified the optimal storage condition, and found that the addition with 5% (w/v) sucrose or trehalose to LLNs could remain their mRNA delivery efficiency for at least three months in the liquid nitrogen storage condition.

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Crossref · 10.1016/j.bioactmat.2020.03.001

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Metadata

Authors
Zhao, P., Hou, X., Yan, J., Du, S., Xue, Y., Li, W., Xiang, G., & Dong, Y.
Affiliations
  • Huazhong University of Science and Technology (CN)
  • The Ohio State University (US)
Corresponding author
  • Xiang, Guangya
  • Dong, Yizhou
Venue
Bioactive Materials
Publisher
Elsevier BV
Published
2020-06
Volume
5
Issue
2
Pages
358-363
ISSN
  • 2097-1192 (ISSN-L)
  • 2452-199X
OpenAlex
W3011313923
Cited by
233
References
34
Citations by year
2026: 17 · 2025: 39 · 2024: 48 · 2023: 53 · 2022: 47 · 2021: 24 · 2020: 4
FWCI
10.38
Type
journal article
Language
en
Open access
Locations
  • Bioactive Materials (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Ohio State University
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
Keywords
mRNA delivery, long-term storage, cryoprotectants, sucrose, trehalose, mannitol, freeze-drying, freezing, liquid nitrogen storage, nanoparticle stability

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