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Long-term storage of lipid-like nanoparticles for mRNA delivery
Open accessAbstract
Lipid-like nanoparticles (LLNs) have been extensively explored for messenger RNA (mRNA) delivery in various biomedical applications. However, the long-term storage of these nanoparticles is still a challenge for their clinical translation. In this study, we investigated a series of conditions for the long-term storage of LLNs with encapsulation of mRNA. We evaluated the stability of LLNs with different concentrations of cryoprotectants (sucrose, trehalose or mannitol) under the conditions of freezing or lyophilization processes. Through in vitro and in vivo mRNA delivery studies, we identified the optimal storage condition, and found that the addition with 5% (w/v) sucrose or trehalose to LLNs could remain their mRNA delivery efficiency for at least three months in the liquid nitrogen storage condition.
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“For example, the addition of 5% (w/v) sucrose or trehalose to lipid nanoparticle–mRNA formulations, stored in liquid nitrogen, allows maintenance of mRNA delivery efficacy for at least 3 months in vivo168.”
From Lipid nanoparticles for mRNA delivery (2021) · cites this paper as [168]
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“Storage conditions of lipid nanoparticle–mRNA formulations are an important consideration for their clinical translation, because storage (aqueous, freezing and lyophilized storage) and the type of cryoprotectants (sucrose, trehalose or mannitol) affect the long-term stability of lipid nanoparticle–mRNA formulations168.”
From Lipid nanoparticles for mRNA delivery (2021) · cites this paper as [168]
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Cites
Systemic RNA delivery to dendritic cells exploits antiviral defence for cancer immunotherapy
Kranz, L. M., Diken, M., Haas, H., Kreiter, S., Loquai, C., Reuter, K. C., Meng, M., Fritz, D., Vascotto, F., Hefesha, H., et al. (2016) · Nature · doi:10.1038/nature18300 · Cited by 1,819
Targeted delivery of RNAi therapeutics with endogenous and exogenous ligand-based mechanisms
Akinc, A., Querbes, W., De, S., Qin, J., Frank-Kamenetsky, M., Jayaprakash, K. N., Jayaraman, M., Rajeev, K. G., Cantley, W. L., Dorkin, J. R., et al. (2010) · Molecular Therapy · doi:10.1038/mt.2010.85 · Cited by 1,261 · cited 1×
“LLNs-mediated mRNA delivery in vivo involves complex interactions with serum proteins [34].”
BackgroundTools for translation: non-viral materials for therapeutic mRNA delivery
Hajj, K. A., & Whitehead, K. A. (2017) · Nature Reviews Materials · doi:10.1038/natrevmats.2017.56 · Cited by 799
An overview of liposome lyophilization and its future potential
Chen, C., Han, D., Cai, C., & Tang, X. (2009) · Journal of Controlled Release · doi:10.1016/j.jconrel.2009.10.024 · Cited by 547
Islam, M. A., Xu, Y., Tao, W., Ubellacker, J. M., Lim, M., Aum, D., Lee, G. Y., Zhou, K., Zope, H., Yu, M., et al. (2018) · Nature Biomedical Engineering · doi:10.1038/s41551-018-0284-0 · Cited by 339
Ball, R., Bajaj, P., & Whitehead, K. (2016) · International Journal of Nanomedicine · doi:10.2147/ijn.s123062 · Cited by 333
Sago, C. D., Lokugamage, M. P., Paunovska, K., Vanover, D. A., Monaco, C. M., Shah, N. N., Gamboa Castro, M., Anderson, S. E., Rudoltz, T. G., Lando, G. N., et al. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1811276115 · Cited by 313
In Vitro and in Vivo mRNA Delivery Using Lipid-Enveloped pH-Responsive Polymer Nanoparticles
Su, X., Fricke, J., Kavanagh, D. G., & Irvine, D. J. (2011) · Molecular Pharmaceutics · doi:10.1021/mp100390w · Cited by 287
An Orthogonal Array Optimization of Lipid-like Nanoparticles for mRNA Delivery in Vivo
Li, B., Luo, X., Deng, B., Wang, J., McComb, D. W., Shi, Y., Gaensler, K. M. L., Tan, X., Dunn, A. L., Kerlin, B. A., et al. (2015) · Nano Letters · doi:10.1021/acs.nanolett.5b03528 · Cited by 261
Self-citationLipid nanoparticles for delivery of messenger RNA to the back of the eye
Patel, S., Ryals, R. C., Weller, K. K., Pennesi, M. E., & Sahay, G. (2019) · Journal of Controlled Release · doi:10.1016/j.jconrel.2019.04.015 · Cited by 260
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Fang, R. M., Sane, P., Sebastiao, I. B., & Bhatnagar, B. S. (2023) · AAPS advances in the pharmaceutical sciences series · doi:10.1007/978-3-031-12634-5_11 · Cited by 3
Luo, D., Zhang, Z., Guo, J., Zhang, J., Huang, A. C. C., Cao, Q., Zheng, J., Yuan, Y., Wang, D., & Cao, Y. (2025) · Advanced Science · doi:10.1002/advs.202502387 · Cited by 2
Zahedipour, F., Gol, T. M., Wisser, S., Ramesh, A., Ureña-Bailén, G., Jaafari, M. R., & Mezger, M. (2025) · Current Research in Translational Medicine · doi:10.1016/j.retram.2025.103545 · Cited by 3
Shi, P., Liu, H., Refaat, A., Nguyen, T., Nguyen, A., Miao, K., Song, Y., Trépout, S., Tabor, R. F., de Campo, L., et al. (2026) · Small · doi:10.1002/smll.202511946 · Cited by 2
Degey, M., Meloni, L., Dede, B., Guerreiro, I., Penoy, N., Sacré, P., Berger, M., Conrard, L., Hendrickx, E., Maquoi, E., et al. (2026) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2026.127032 · Cited by 2
Critical chemistry manufacturing and controls considerations for mRNA lipid nanoparticle translation
Liu, L., Wang, Y., Gao, H., Ruan, J., Lu, W., Liu, T., Li, X., Zhang, G., & Xiao, Y. (2026) · Discover Nano · doi:10.1186/s11671-026-04864-4 · Cited by 3
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Potupchik, T. V., Kucherenko, A. M., Generalov, S., Alikin, Y., Spitsyn, A., Ermolaev, V., Evert, L., Nikolova, S., Sidorova, V., Kurdova, L., et al. (2026) · Problems of Biological Medical and Pharmaceutical Chemistry · doi:10.29296/25877313-2026-04-01 · Cited by 2
Dowaidar, M. (2021) · OSF Preprints (OSF Preprints) · doi:10.31219/osf.io/9vxrb · Cited by 2
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Chen, X., Fang, J., Ge, X., Li, M., Jiang, F., Hong, L., & Liu, Z. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.09.01.610730 · Cited by 2
Chen, X., Ye, Y., Li, M., Zuo, T., Xie, Z., Ke, Y., Cheng, H., Hong, L., & Liu, Z. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.09.27.614859 · Cited by 2
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Arpagaus, C. (2023) · Springer eBooks · doi:10.1007/978-3-031-24323-3_6 · Cited by 2
Nanoparticle‐Based mRNA Vaccines
Nalla, L. V., Gajula, S. N. R., & Chavda, V. P. (2024) · Wiley eBooks · doi:10.1002/9781394175482.ch8 · Cited by 1
Jackson, N. A. C. (2022) · RNA technologies · doi:10.1007/978-3-031-08415-7_2
Kimura, Y., Osuda, H., & Hara, M. (2023) · Food Bioscience · doi:10.1016/j.fbio.2023.103276
(2024) · Elsevier eBooks · doi:10.1016/b978-0-443-28934-7.15001-0
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Ruiz-Matus, S., Andraca, A., & Goldstein, P. (2025) · Essential Chem · doi:10.1080/28378083.2025.2464144
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Fule, R. A., Rangurwar, A. S., Nagdeve, A., & Nagpure, P. (2025) · Springer eBooks · doi:10.1007/978-981-95-0221-9_7 · Cited by 1
Du, C., Xiong, C., Wang, Y., Hu, Z. H., Xing, R., & Fang, W.-J. (2026) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.5c01450
Vectorisation d'ARN par voie orale et parentérale
Stalder, T. (2024) · theses.fr (ABES) · doi:10.70675/d509592fzbb96z4f17zb286z5040de22fd65
Ishikawa, U., Mabrouk, M. T., & Yang, Y. (2026) · ACS Nano Medicine · doi:10.1021/acsnanomed.5c00166
Chowdhury, A., Silva-Santos, A. R., Azevedo, A. M., & Rathore, A. S. (2026) · Pharmaceutical Research · doi:10.1007/s11095-026-04132-1
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Lipid Nanoparticles at the Crossroads of Chemistry and Medicine: A Tutorial
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Metadata
- Authors
- Zhao, P., Hou, X., Yan, J., Du, S., Xue, Y., Li, W., Xiang, G., & Dong, Y.
- Affiliations
- Huazhong University of Science and Technology (CN)
- The Ohio State University (US)
- Corresponding author
- Xiang, Guangya
- Dong, Yizhou
- Venue
- Bioactive Materials
- Publisher
- Elsevier BV
- Published
- 2020-06
- Volume
- 5
- Issue
- 2
- Pages
- 358-363
- ISSN
- 2097-1192 (ISSN-L)
- 2452-199X
- PMC
- PMC7078456
- OpenAlex
- W3011313923
- Cited by
- 233
- References
- 34
- Citations by year
- 2026: 17 · 2025: 39 · 2024: 48 · 2023: 53 · 2022: 47 · 2021: 24 · 2020: 4
- FWCI
- 10.38
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Bioactive Materials (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ohio State University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
- Keywords
- mRNA delivery, long-term storage, cryoprotectants, sucrose, trehalose, mannitol, freeze-drying, freezing, liquid nitrogen storage, nanoparticle stability
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