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Cited by 147Bone Tissue Engineering MaterialsPeriodontal Regeneration and TreatmentsElectrospun Nanofibers in Biomedical Applications

Cerium oxide nanoparticles loaded nanofibrous membranes promote bone regeneration for periodontal tissue engineering

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Ren, Shuangshuang, Zhou, Yi, Zheng, Kai, Xu, Xuanwen, Yang, Jie, Wang, Xiaoyu, Miao, Leiying, Wei, Hui +1 more·2022·Bioactive Materials
DOI 10.1016/j.bioactmat.2021.05.037PMC PMC8379477
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Abstract

Bone regeneration is a crucial part in the treatment of periodontal tissue regeneration, in which new attempts come out along with the development of nanomaterials. Herein, the effect of cerium oxide nanoparticles (CeO2 NPs) on the cell behavior and function of human periodontal ligament stem cells (hPDLSCs) was investigated. Results of CCK-8 and cell cycle tests demonstrated that CeO2 NPs not only had good biocompatibility, but also promoted cell proliferation. Furthermore, the levels of alkaline phosphatase activity, mineralized nodule formation and expressions of osteogenic genes and proteins demonstrated CeO2 NPs could promote osteogenesis differentiation of hPDLSCs. Then we chose electrospinning to fabricate fibrous membranes containing CeO2 NPs. We showed that the composite membranes improved mechanical properties as well as realized release of CeO2 NPs. We then applied the composite membranes to in vivo study in rat cranial defect models. Micro-CT and histopathological evaluations revealed that nanofibrous membranes with CeO2 NPs further accelerated new bone formation. Those exciting results demonstrated that CeO2 NPs and porous membrane contributed to osteogenic ability, and CeO2 NPs contained electrospun membrane may be a promising candidate material for periodontal bone regeneration.

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  • CeNPs promote angiogenesis and osteogenesis by alleviating oxidative stress and modulating osteocyte signaling via the TGF-β/BMP pathway.

    “Additionally, CeNPs promote angiogenesis and osteogenesis by alleviating oxidative stress and modulating osteocyte signaling via the TGF-β/BMP pathway22,23.”

    From Microenvironment-responsive multifunctional enzyme-linked hydrogel for diabetic bone defect regeneration. (2025) · cites this paper as [22]

    The Results (In vitro effects of CeO2 NPs on osteogenic differentiation; page unavailable) report increased osteogenic differentiation of periodontal ligament stem cells, and the Introduction describes angiogenic potential. The Discussion only proposes that ROS scavenging might protect stem cells; measuring BMP-2 expression does not establish TGF-β/BMP pathway signaling in osteocytes or show that oxidative-stress relief caused angiogenesis and osteogenesis. (LLM, conf=0.87)

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Cited by

Sorted by citations

Authors
Ren, S., Zhou, Y., Zheng, K., Xu, X., Yang, J., Wang, X., Miao, L., Wei, H., & Xu, Y.
Affiliations
  • Nanjing Medical University (CN)
  • Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
  • Nanjing University (CN)
  • State Key Laboratory of Analytical Chemistry for Life Science (CN)
Corresponding author
  • Miao, Leiying
  • Wei, Hui
  • Xu, Yan
ORCID
Venue
Bioactive Materials
Publisher
Elsevier BV
Published
2022-01
Volume
7
Pages
242-253
ISSN
  • 2097-1192 (ISSN-L)
  • 2452-199X
DOI
10.1016/j.bioactmat.2021.05.037
PMC
PMC8379477
OpenAlex
W3170983871
Cited by
147
References
3
Citations by year
2026: 14 · 2025: 33 · 2024: 38 · 2023: 38 · 2022: 21 · 2021: 3
FWCI
7.47
Type
journal article
Language
en
Open access
Licence
Locations
  • Bioactive Materials (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Natural Science Foundation of Jiangsu Province (20200665)
  • Priority Academic Program Development of Jiangsu Higher Education Institutions
  • National Natural Science Foundation of China (81771074, 82001049)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
  • Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
Keywords
cerium oxide nanoparticles, nanofibrous membranes, periodontal tissue engineering, periodontal bone regeneration, periodontal ligament stem cells, osteogenic differentiation, electrospinning, biocompatibility, cranial defects, bone formation, osteogenic gene expression, alkaline phosphatase activity, membrane mechanics, nanoparticle release

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