Adhesion and proliferation of OCT-1 osteoblast-like cells on micro- and nano-scale topography structured poly(l-lactide)
Abstract
The impact of the surface topography of polylactone-type polymer on cell adhesion was to be concerned because the micro-scale texture of a surface can provide a significant effect on the adhesion behavior of cells on the surface. Especially for the application of tissue engineering scaffold, the pore size could have an influence on cell in-growth and subsequent proliferation. Micro-fabrication technology was used to generate specific topography to investigate the relationship between the cells and surface. In this study the pits-patterned surfaces of polystyrene (PS) film with diameters 2.2 and 0.45 microm were prepared by phase-separation, and the corresponding scale islands-patterned PLLA surface was prepared by a molding technique using the pits-patterned PS as a template. The adhesion and proliferation behavior of OCT-1 osteoblast-like cells morphology on the pits- and islands-patterned surface were characterized by SEM observation, cell attachment efficiency measurement and MTT assay. The results showed that the cell adhesion could be enhanced on PLLA and PS surface with nano-scale and micro-scale roughness compared to the smooth surfaces of the PLLA and PS. The OCT-1 osteoblast-like cells could grow along the surface with two different size islands of PLLA and grow inside the micro-scale pits of the PS. However, the proliferation of cells on the micro- and nano-scale patterned surface has not been enhanced compared with the controlled smooth surface.
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Cells in the body perform bioactivities in response to stimulation from a highly complex, 3D microenvironment.
“Cells in our body perform bioactivities in response to the stimulation from a highly complex, 3D microenvironment (15,98,112).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [112]
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- Authors
- Wan, Y., Wang, Y., Liu, Z., Qu, X., Han, B., Bei, J., & Wang, S.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Wang, Shenguo
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2005-07
- Volume
- 26
- Issue
- 21
- Pages
- 4453-4459
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2019520860
- Cited by
- 342
- References
- 18
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 6 · 2023: 12 · 2022: 8 · 2021: 8 · 2020: 7 · 2019: 11 · 2018: 16 · 2017: 14 · 2016: 14 · 2015: 19 · 2014: 17 · 2013: 27 · 2012: 27
- FWCI
- 12.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- cell adhesion, cell proliferation, poly(L-lactic acid), surface topography, polystyrene template, phase separation, tissue engineering scaffolds, surface roughness
- MeSH terms
- Animals, Biocompatible Materials, Cell Adhesion, Cell Line, Humans, Materials Testing, Osteoblasts, Polyesters, Surface Properties, Cell Culture Techniques, Tissue Engineering, Cell Size, Cell Proliferation, Nanostructures
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