The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds
Open accessAbstract
Biomaterials derived from silk fibroin prepared by aqueous (AB) and organic (HFIP) solvent-based processes, along with collagen (COL) and poly-lactic acid (PLA)-based scaffolds were studied in vitro and in vivo for their utility in adipose tissue engineering strategies. For in vitro studies, human bone marrow and adipose-derived mesenchymal stem cells (hMSCs and hASCs) were seeded on the various biomaterials and cultured for 21 days in the presence of adipogenic stimulants (AD) or maintained as noninduced controls. Alamar Blue analysis revealed each biomaterial supported initial attachment of hMSCs and hASCs to similar levels for all matrices except COL in which higher levels were observed. hASCs and hMSCs cultured on all biomaterials in the presence of AD showed significant upregulation of adipogenic mRNA transcript levels (LPL, GLUT4, FABP4, PPARgamma, adipsin, ACS) to similar extents when compared to noninduced controls. Similarly Oil-Red O analysis of hASC or hMSC-seeded scaffolds displayed substantial amounts of lipid accumulating adipocytes following cultivation with AD. The data revealed AB and HFIP scaffolds supported similar extents of lipid accumulating cells while PLA and COL scaffolds qualitatively displayed lower and higher extents by comparison, respectively. Following a 4-week implantation period in a rat muscle pouch defect model, both AB and HFIP scaffolds supported in vivo adipogenesis either alone or seeded with hASCs or hMSCs as assessed by Oil-Red O analysis, however the presence of exogenous cell sources substantially increased the extent and frequency of adipogenesis observed. In contrast, COL and PLA scaffolds underwent rapid scaffold degradation and were irretrievable following the implantation period. The results suggest that macroporous 3D AB and HFIP silk fibroin scaffolds offer an important platform for cell-based adipose tissue engineering applications, and in particular, provide longer-term structural integrity to promote the maintenance of soft tissue in vivo.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Although the silk protein-based scaffold does not contain any cell-specific signal expression in its primary sequence, it provides a suitable matrix for supporting the proliferation and adhesion of mesenchymal stem cells [31,32].”
From Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review (2025) · cites this paper as [32]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
Altman, G. H., Diaz, F., Jakuba, C., Calabro, T., Horan, R. L., Chen, J., Lu, H., Richmond, J., & Kaplan, D. L. (2003) · Biomaterials · doi:10.1016/s0142-9612(02)00353-8 · Cited by 3,458
Self-citationPPARγ Is Required for the Differentiation of Adipose Tissue In Vivo and In Vitro
Rosen, E. D., Sarraf, P., Troy, A. E., Bradwin, G., Moore, K. J., Milstone, D. S., Spiegelman, B. M., & Mortensen, R. M. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80211-7 · Cited by 2,081
Principles of Tissue Engineering
(2014) · Elsevier eBooks · doi:10.1016/c2011-0-07193-4 · Cited by 1,912
C/EBPα induces adipogenesis through PPARγ: a unified pathwayPreprint
Rosen, E. D., Hsu, C.-H., Wang, X., Sakai, S., Freeman, M. W., Gonzalez, F. J., & Spiegelman, B. M. (2002) · Genes & Development · doi:10.1101/gad.948702 · Cited by 1,497
Control of the peroxisomal β-oxidation pathway by a novel family of nuclear hormone receptors
Dreyer, C., Krey, G. D., Keller, H., Givel, F., Helftenbein, G., & Wahli, W. (1992) · Cell · doi:10.1016/0092-8674(92)90031-7 · Cited by 1,393
PPARγ Mediates High-Fat Diet–Induced Adipocyte Hypertrophy and Insulin Resistance
Kubota, N., Terauchi, Y., Miki, H., Tamemoto, H., Yamauchi, T., Komeda, K., Satoh, S., Nakano, R., Ishii, C., 杉山, 拓., et al. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80210-5 · Cited by 1,351
Schoonjans, K., Staels, B., & Auwerx, J. H. (1996) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(96)00066-5 · Cited by 1,045
Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin
Kim, U., Park, J. H., Kim, H. J., Wada, M., & Kaplan, D. L. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.07.044 · Cited by 979
Self-citationPorous 3-D Scaffolds from Regenerated Silk Fibroin
Nazarov, R., Jin, H., & Kaplan, D. L. (2004) · Biomacromolecules · doi:10.1021/bm034327e · Cited by 888
Self-citationThe inflammatory responses to silk films in vitro and in vivo
Meinel, L., Hofmann, S., Karageorgiou, V., Kirker‐Head, C. A., McCool, J., Gronowicz, G., Zichner, L., Langer, R., Vunjak‐Novakovic, G., & Kaplan, D. L. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.02.047 · Cited by 785
Self-citationIn vitro degradation of silk fibroin
Horan, R. L., Antle, K. A., Collette, A. L., Wang, Y., Huang, J., Moreau, J. E., Volloch, V., Kaplan, D. L., & Altman, G. H. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.09.020 · Cited by 741
Self-citationElectrospinning Bombyx mori Silk with Poly(ethylene oxide)
Jin, H., Fridrikh, S. V., Rutledge, G. C., & Kaplan, D. L. (2002) · Biomacromolecules · doi:10.1021/bm025581u · Cited by 715
Self-citationCell differentiation by mechanical stress
Altman, G. H., Horan, R. L., Martín, I., Farhadi, J., Stark, P. R. H., Volloch, V., Richmond, J. C., Vunjak‐Novakovic, G., & Kaplan, D. L. (2001) · The FASEB Journal · doi:10.1096/fj.01-0656fje · Cited by 670
Self-citationPPARγ, the ultimate thrifty gene
Auwerx, J. (1999) · Diabetologia · doi:10.1007/s001250051268 · Cited by 612
Principles of Tissue Engineering
Pegg, D. E. (1999) · Cryobiology · doi:10.1006/cryo.1999.2214 · Cited by 566
Meinel, L., Karageorgiou, V., Fajardo, R., Snyder, B. D., Patil, V. R. S., Zichner, L., Kaplan, D. L., Langer, R., & Vunjak‐Novakovic, G. (2003) · Annals of Biomedical Engineering · doi:10.1023/b:abme.0000007796.48329.b4 · Cited by 561
Self-citationSignaling Mechanisms That Regulate Glucose Transport
Czech, M., & Corvera, S. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.4.1865 · Cited by 519
The Adipocyte as an Endocrine Cell
Halberg, N., Asterholm, I. W., & Scherer, P. E. (2008) · Endocrinology and Metabolism Clinics of North America · doi:10.1016/j.ecl.2008.07.002 · Cited by 442
Cartilage tissue engineering with silk scaffolds and human articular chondrocytes
Wang, Y., Blasioli, D. J., Kim, H.-J., Kim, H. S., & Kaplan, D. L. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.03.050 · Cited by 420
Self-citationHuman adipsin is identical to complement factor D and is expressed at high levels in adipose tissue.
White, R. T., Damm, D. L., Hancock, N., Rosen, B. S., Lowell, B. B., Usher, P., Flier, J. S., & Spiegelman, B. M. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50409-4 · Cited by 409
Frank, O., Heim, M., Jakob, M., Barbero, A., Schäfer, D., Bendik, I., Dick, W., Heberer, M., & Martín, I. (2002) · Journal of Cellular Biochemistry · doi:10.1002/jcb.10174 · Cited by 370
Engineering bone‐like tissue in vitro using human bone marrow stem cells and silk scaffolds
Meinel, L., Karageorgiou, V., Hofmann, S., Fajardo, R., Snyder, B. D., Li, C., Zichner, L., Langer, R., Vunjak‐Novakovic, G., & Kaplan, D. L. (2004) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30117 · Cited by 349
Self-citationEngineering cartilage‐like tissue using human mesenchymal stem cells and silk protein scaffolds
Meinel, L., Hofmann, S., Karageorgiou, V., Zichner, L., Langer, R., Kaplan, D. L., & Vunjak‐Novakovic, G. (2004) · Biotechnology and Bioengineering · doi:10.1002/bit.20252 · Cited by 310
Self-citationFatty Acid Binding Proteins—The Evolutionary Crossroads of Inflammatory and Metabolic Responses
Makowski, L., & Hotamışlıgil, G. S. (2004) · Journal of Nutrition · doi:10.1093/jn/134.9.2464s · Cited by 309
Preadipocyte Seeded PLGA Scaffolds for Adipose Tissue Engineering
Patrick, C., Chauvin, P., Hobley, J., & Reece, G. P. (1999) · Tissue Engineering · doi:10.1089/ten.1999.5.139 · Cited by 283
Molecular regulation of adipocyte differentiation
Cowherd, R. M., Lyle, R. E., & McGehee, R. E. (1999) · Seminars in Cell and Developmental Biology · doi:10.1006/scdb.1998.0276 · Cited by 270
Frontiers in Tissue Engineering
(1998) · Elsevier eBooks · doi:10.1016/b978-0-08-042689-1.x5001-8 · Cited by 267
Kimura, Y., Ozeki, M., Inamoto, T., & Tabata, Y. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00049-8 · Cited by 257
Tissue engineering strategies for adipose tissue repair
Patrick, C. (2001) · The Anatomical Record · doi:10.1002/ar.1113 · Cited by 255
Human preadipocytes seeded on freeze-dried collagen scaffolds investigated in vitro and in vivo
von Heimburg, D., Zachariah, S., Kühling, H., Heschel, I., Schoof, H., Hafemann, B., & Pallua, N. (2001) · Biomaterials · doi:10.1016/s0142-9612(00)00186-1 · Cited by 229
Long-Term Implantation of Preadipocyte-Seeded PLGA Scaffolds
Patrick, C., Zheng, B., Johnston, C. M., & Reece, G. P. (2002) · Tissue Engineering · doi:10.1089/107632702753725049 · Cited by 210
Patrick, C. (2000) · Seminars in Surgical Oncology · doi:10.1002/1098-2388(200010/11)19:3 · Cited by 204
Mauney, J. R., Jaquiéry, C., Volloch, V., Heberer, M., Martín, I., & Kaplan, D. L. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.08.020 · Cited by 183
Self-citationApplication and evaluation of the alamarblue assay for cell growth and survival of fibroblasts
Voytik‐Harbin, S. L., Brightman, A. O., Waisner, B. Z., Lamar, C. H., & Badylak, S. F. (1998) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-998-0130-x · Cited by 167
Samuelsson, L., Strömberg, K., Vikman, K., Bjursell, G., & Enerbäck, S. (1991) · The EMBO Journal · doi:10.1002/j.1460-2075.1991.tb04948.x · Cited by 160
Lazar, M. A. (2004) · Biochimie · doi:10.1016/j.biochi.2004.10.021 · Cited by 138
Engineering of volume-stable adipose tissues
Cho, S., Kim, S. S., Rhie, J. W., Cho, H. M., Choi, C. Y., & Kim, B. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.09.013 · Cited by 135
The adipocyte as an endocrine cell1
Miner, J. L. (2004) · Journal of Animal Science · doi:10.2527/2004.823935x · Cited by 131
Mauney, J. R., Volloch, V., & Kaplan, D. L. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.03.024 · Cited by 126
Self-citationBioreactor cultivation of osteochondral grafts
Vunjak‐Novakovic, G., Meinel, L., Altman, G. H., & Kaplan, D. L. (2005) · Orthodontics and Craniofacial Research · doi:10.1111/j.1601-6343.2005.00334.x · Cited by 120
Self-citationNeubauer, M., Hacker, M. C., Bauer‐Kreisel, P., Weiser, B., Fischbach, C., Schulz‐Siegmund, M., Göpferich, A. M., & Blunk, T. (2005) · Tissue Engineering · doi:10.1089/ten.2005.11.1840 · Cited by 119
Fischbach, C., Spruß, T., Weiser, B., Neubauer, M., Becker, C., Hacker, M. C., Göpferich, A. M., & Blunk, T. (2004) · Experimental Cell Research · doi:10.1016/j.yexcr.2004.05.036 · Cited by 115
Adipogenic differentiation of adipose tissue derived adult stem cells in nude mouse
Choi, Y. S., Cha, S. M., Lee, Y. Y., Kwon, S. W., Park, C. J., & Kim, M. (2006) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2006.04.128 · Cited by 104
Engineered Adipose Tissue Supplied by Functional Microvessels
Borges, J., Mueller, M. C., Padron, N. T., Tegtmeier, F. T., Lang, E., & Stark, G. B. (2003) · Tissue Engineering · doi:10.1089/10763270360728170 · Cited by 88
Kang, X., Xie, Y., & Kniss, D. A. (2005) · Tissue Engineering · doi:10.1089/ten.2005.11.458 · Cited by 80
Three-Dimensional in Vitro Model of Adipogenesis: Comparison of Culture Conditions
Fischbach, C., Seufert, J., Staiger, H., Hacker, M. C., Neubauer, M., Göpferich, A. M., & Blunk, T. (2004) · Tissue Engineering · doi:10.1089/107632704322791862 · Cited by 71
De novo adipose formation in a vascularized engineered construct
Walton, R. L., Beahm, E. K., & Wu, L. C. (2004) · Microsurgery · doi:10.1002/micr.20056 · Cited by 70
Induction of preadipocyte differentiation by mature fat cells in the rat.
Shillabeer, G., Forden, J., & Lau, D. C. W. (1989) · Journal of Clinical Investigation · doi:10.1172/jci114177 · Cited by 62
Expression and regulation of pOb24 and lipoprotein lipase genes during adipose conversion
Dani, C., Amri, E., Bertrand, B., Enerbäck, S., Bjursell, G., Grimaldi, P. A., & Ailhaud, G. P. (1990) · Journal of Cellular Biochemistry · doi:10.1002/jcb.240430202 · Cited by 56
Fatty acid binding proteins stabilize leukotriene A4
Zimmer, J. S. D., Dyckes, D. F., Bernlohr, D., & Murphy, R. C. (2004) · Journal of Lipid Research · doi:10.1194/jlr.m400240-jlr200 · Cited by 55
Lipografting: Autologous Fat Grafting for Total Facial Rejuvenation
Tzikas, T. L. (2004) · Facial Plastic Surgery · doi:10.1055/s-2004-861754 · Cited by 50
Kim, C.-S., Kawada, T., Yoo, H., Kwon, B., & Yu, R. (2003) · FEBS Letters · doi:10.1016/s0014-5793(03)00728-2 · Cited by 45
Shimomura, I., Tokunaga, K., Jiao, S., Funahashi, T., Keno, Y., Kobatake, T., Kotani, K., Suzuki, H., Yamamoto, T. T., Tarui, S., et al. (1992) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(92)90086-b · Cited by 42
Diabetes with partial lipodystrophy following sclerodermatous chronic graft vs. host disease
Rooney, D. P., & Ryan, M. F. (2006) · Diabetic Medicine · doi:10.1111/j.1464-5491.2006.01855.x · Cited by 34
Lee, K. Y., Halberstadt, C. R., Holder, W. D., & Mooney, D. J. (2000) · Elsevier eBooks · doi:10.1016/b978-012436630-5/50035-0 · Cited by 26
Powers, M. P., & Bosker, H. (1996) · Journal of Oral and Maxillofacial Surgery · doi:10.1016/s0278-2391(96)90386-9 · Cited by 19
Flagged first, then by citations
Cited by
Sorted by citations
Materials fabrication from Bombyx mori silk fibroin
Rockwood, D. N., Preda, R. C., Yücel, T., Wang, X., Lovett, M. L., & Kaplan, D. L. (2011) · Nature Protocols · doi:10.1038/nprot.2011.379 · Cited by 3,149
Self-citationBiopolymer-Based Hydrogels As Scaffolds for Tissue Engineering Applications: A Review
Vlierberghe, S. V., Dubruel, P., & Schacht, E. H. (2011) · Biomacromolecules · doi:10.1021/bm200083n · Cited by 1,719
Biodegradation of Silk Biomaterials
Cao, Y., & Wang, B. (2009) · International Journal of Molecular Sciences · doi:10.3390/ijms10041514 · Cited by 719
Advances in 3D cell culture technologies enabling tissue‐like structures to be created in vitro
Knight, E., & Przyborski, S. A. (2014) · Journal of Anatomy · doi:10.1111/joa.12257 · Cited by 569
Biomedical applications of chemically-modified silk fibroin
Murphy, A. R., & Kaplan, D. L. (2009) · Journal of Materials Chemistry · doi:10.1039/b905802h · Cited by 522
Self-citation
Metadata
- Authors
- Mauney, J. R., Nguyen, T., Gillen, K., Kirker-Head, C., Gimble, J. M., & Kaplan, D. L.
- Affiliations
- Tufts University (US)
- Pennington Biomedical Research Center (US)
- Corresponding author
- Kaplan, David L.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2007-12
- Volume
- 28
- Issue
- 35
- Pages
- 5280-5290
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC2695965
- OpenAlex
- W2095560028
- Cited by
- 373
- References
- 57
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 9 · 2023: 10 · 2022: 12 · 2021: 22 · 2020: 11 · 2019: 21 · 2018: 16 · 2017: 29 · 2016: 25 · 2015: 25 · 2014: 35 · 2013: 34 · 2012: 30
- FWCI
- 9.15
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Biomedical Imaging and Bioengineering (R01EB003210, P41EB002520)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- adipose tissue engineering, silk fibroin scaffolds, adipose-derived stem cells, bone marrow mesenchymal stem cells, adipogenesis, collagen scaffolds, polylactic acid scaffolds, scaffold degradation, cell seeding, macroporous scaffolds
- MeSH terms
- Adipose Tissue, Adult, Animals, Biocompatible Materials, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Fibroins, Humans, Male, Rats, Nude, Bombyx, Tissue Engineering, Silk, Rats, Tissue Scaffolds, Mesenchymal Stem Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
