The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.
Engineering adipose-like tissue in vitro and in vivo utilizing human bone marrow and adipose-derived mesenchymal stem cells with silk fibroin 3D scaffolds
Open accessAbstract
Biomaterials derived from silk fibroin prepared by aqueous (AB) and organic (HFIP) solvent-based processes, along with collagen (COL) and poly-lactic acid (PLA)-based scaffolds were studied in vitro and in vivo for their utility in adipose tissue engineering strategies. For in vitro studies, human bone marrow and adipose-derived mesenchymal stem cells (hMSCs and hASCs) were seeded on the various biomaterials and cultured for 21 days in the presence of adipogenic stimulants (AD) or maintained as noninduced controls. Alamar Blue analysis revealed each biomaterial supported initial attachment of hMSCs and hASCs to similar levels for all matrices except COL in which higher levels were observed. hASCs and hMSCs cultured on all biomaterials in the presence of AD showed significant upregulation of adipogenic mRNA transcript levels (LPL, GLUT4, FABP4, PPARgamma, adipsin, ACS) to similar extents when compared to noninduced controls. Similarly Oil-Red O analysis of hASC or hMSC-seeded scaffolds displayed substantial amounts of lipid accumulating adipocytes following cultivation with AD. The data revealed AB and HFIP scaffolds supported similar extents of lipid accumulating cells while PLA and COL scaffolds qualitatively displayed lower and higher extents by comparison, respectively. Following a 4-week implantation period in a rat muscle pouch defect model, both AB and HFIP scaffolds supported in vivo adipogenesis either alone or seeded with hASCs or hMSCs as assessed by Oil-Red O analysis, however the presence of exogenous cell sources substantially increased the extent and frequency of adipogenesis observed. In contrast, COL and PLA scaffolds underwent rapid scaffold degradation and were irretrievable following the implantation period. The results suggest that macroporous 3D AB and HFIP silk fibroin scaffolds offer an important platform for cell-based adipose tissue engineering applications, and in particular, provide longer-term structural integrity to promote the maintenance of soft tissue in vivo.
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“Although the silk protein-based scaffold does not contain any cell-specific signal expression in its primary sequence, it provides a suitable matrix for supporting the proliferation and adhesion of mesenchymal stem cells [31,32].”
From Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review (2025) · cites this paper as [32]
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Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
Altman, G. H., Diaz, F., Jakuba, C., Calabro, T., Horan, R. L., Chen, J., Lu, H., Richmond, J., & Kaplan, D. L. (2003) · Biomaterials · doi:10.1016/s0142-9612(02)00353-8 · Cited by 3,458
Self-citationPPARγ Is Required for the Differentiation of Adipose Tissue In Vivo and In Vitro
Rosen, E. D., Sarraf, P., Troy, A. E., Bradwin, G., Moore, K. J., Milstone, D. S., Spiegelman, B. M., & Mortensen, R. M. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80211-7 · Cited by 2,081
Principles of Tissue Engineering
(2014) · Elsevier eBooks · doi:10.1016/c2011-0-07193-4 · Cited by 1,912
C/EBPα induces adipogenesis through PPARγ: a unified pathwayPreprint
Rosen, E. D., Hsu, C.-H., Wang, X., Sakai, S., Freeman, M. W., Gonzalez, F. J., & Spiegelman, B. M. (2002) · Genes & Development · doi:10.1101/gad.948702 · Cited by 1,497
Control of the peroxisomal β-oxidation pathway by a novel family of nuclear hormone receptors
Dreyer, C., Krey, G. D., Keller, H., Givel, F., Helftenbein, G., & Wahli, W. (1992) · Cell · doi:10.1016/0092-8674(92)90031-7 · Cited by 1,393
PPARγ Mediates High-Fat Diet–Induced Adipocyte Hypertrophy and Insulin Resistance
Kubota, N., Terauchi, Y., Miki, H., Tamemoto, H., Yamauchi, T., Komeda, K., Satoh, S., Nakano, R., Ishii, C., 杉山, 拓., et al. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80210-5 · Cited by 1,351
Schoonjans, K., Staels, B., & Auwerx, J. H. (1996) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(96)00066-5 · Cited by 1,045
Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin
Kim, U., Park, J. H., Kim, H. J., Wada, M., & Kaplan, D. L. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.07.044 · Cited by 979
Self-citationPorous 3-D Scaffolds from Regenerated Silk Fibroin
Nazarov, R., Jin, H., & Kaplan, D. L. (2004) · Biomacromolecules · doi:10.1021/bm034327e · Cited by 888
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Materials fabrication from Bombyx mori silk fibroin
Rockwood, D. N., Preda, R. C., Yücel, T., Wang, X., Lovett, M. L., & Kaplan, D. L. (2011) · Nature Protocols · doi:10.1038/nprot.2011.379 · Cited by 3,149
Self-citationBiopolymer-Based Hydrogels As Scaffolds for Tissue Engineering Applications: A Review
Vlierberghe, S. V., Dubruel, P., & Schacht, E. H. (2011) · Biomacromolecules · doi:10.1021/bm200083n · Cited by 1,719
Biodegradation of Silk Biomaterials
Cao, Y., & Wang, B. (2009) · International Journal of Molecular Sciences · doi:10.3390/ijms10041514 · Cited by 719
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Knight, E., & Przyborski, S. A. (2014) · Journal of Anatomy · doi:10.1111/joa.12257 · Cited by 569
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Murphy, A. R., & Kaplan, D. L. (2009) · Journal of Materials Chemistry · doi:10.1039/b905802h · Cited by 522
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Metadata
- Authors
- Mauney, J. R., Nguyen, T., Gillen, K., Kirker-Head, C., Gimble, J. M., & Kaplan, D. L.
- Affiliations
- Tufts University (US)
- Pennington Biomedical Research Center (US)
- Corresponding author
- Kaplan, David L.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2007-12
- Volume
- 28
- Issue
- 35
- Pages
- 5280-5290
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC2695965
- OpenAlex
- W2095560028
- Cited by
- 373
- References
- 57
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 9 · 2023: 10 · 2022: 12 · 2021: 22 · 2020: 11 · 2019: 21 · 2018: 16 · 2017: 29 · 2016: 25 · 2015: 25 · 2014: 35 · 2013: 34 · 2012: 30
- FWCI
- 9.15
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institute of Biomedical Imaging and Bioengineering (R01EB003210, P41EB002520)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- adipose tissue engineering, silk fibroin scaffolds, adipose-derived stem cells, bone marrow mesenchymal stem cells, adipogenesis, collagen scaffolds, polylactic acid scaffolds, scaffold degradation, cell seeding, macroporous scaffolds
- MeSH terms
- Adipose Tissue, Adult, Animals, Biocompatible Materials, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Fibroins, Humans, Male, Rats, Nude, Bombyx, Tissue Engineering, Silk, Rats, Tissue Scaffolds, Mesenchymal Stem Cells
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