Modification of silk fibroin using diazonium coupling chemistry and the effects on hMSC proliferation and differentiation
Open accessAbstract
A simple chemical modification method using diazonium coupling chemistry was developed to tailor the structure and hydrophilicity of silk fibroin protein. The extent of modification using several aniline derivatives was characterized using UV-vis and 1H NMR spectroscopies, and the resulting protein structure was analyzed with ATR-FTIR spectroscopy. Introduction of hydrophobic functional groups facilitated rapid conversion of the protein from a random coil to a beta-sheet structure, while addition of hydrophilic groups inhibited this process. hMSCs were grown on these modified silks to assess the biocompatibility of these materials. The hydrophilicity of the silk derivatives was found to affect the growth rate and morphology, but hMSCs were able to attach, proliferate and differentiate into an osteogenic lineage on all of the silk derivatives.
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Exposing silk fibroin membranes to water or methanol vapor improves the stability of the silk fibroin film by increasing β-sheet content.
“By exposing the silk fibroin membranes to water or methanol vapor, the stability of the silk fibroin film can also be improved, which will increase the β-sheet content of the silk fibroin, thereby enhancing the stability of the silk fibroin film [81].”
From Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review (2025) · cites this paper as [81]
The Results (§3.4; page unavailable) say methanol treatment induces β-sheet formation in silk films. The Methods (§2.4; page unavailable) say soaking the films in 70% methanol makes them insoluble in water. The paper does not establish that exposure to water vapor or methanol vapor has these effects; its film treatment was a methanol-solution soak. (LLM, conf=0.88)
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“By exposing the silk fibroin membranes to water or methanol vapor, the stability of the silk fibroin film can also be improved, which will increase the β -sheet content of the silk fibroin, thereby enhancing the stability of the silk fibroin film [81].”
- Authors
- Murphy, A. R., John, P. S., & Kaplan, D. L.
- Affiliations
- Tufts University (US)
- Corresponding author
- Kaplan, David L.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2008-07
- Volume
- 29
- Issue
- 19
- Pages
- 2829-2838
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC2698958
- OpenAlex
- W2033592937
- Cited by
- 277
- References
- 56
- Citations by year
- 2026: 13 · 2025: 14 · 2024: 9 · 2023: 16 · 2022: 21 · 2021: 17 · 2020: 21 · 2019: 16 · 2018: 19 · 2017: 14 · 2016: 15 · 2015: 20 · 2014: 20 · 2013: 12 · 2012: 15
- FWCI
- 5.03
- Type
- journal article
- Language
- en
- Update notices
- erratum (2008-11-01)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (K12GM074869)
- National Institute of Biomedical Imaging and Bioengineering (P41EB002520)
- National Institute of Dental and Craniofacial Research (R01DE013405, R01DE017207)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Keywords
- silk fibroin, diazonium salt coupling, chemical modification, aniline derivatives, hydrophilicity, protein secondary structure, β-sheet formation, hydrophobic functional groups, human mesenchymal stem cells, cell proliferation, osteogenic differentiation, biocompatibility, silk-based biomaterials
- MeSH terms
- Cell Differentiation, Diazonium Compounds, Fibroins, Humans, Spectrophotometry, Ultraviolet, Spectroscopy, Fourier Transform Infrared, Nuclear Magnetic Resonance, Biomolecular, Reverse Transcriptase Polymerase Chain Reaction, Silk, Cell Proliferation, Mesenchymal Stem Cells
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