Mesenchymal stem cell differentiation to neuronal cells on electrospun nanofibrous substrates for nerve tissue engineering
Abstract
Bone marrow Mesenchymal stem cells capable of differentiating into neuronal cells on engineered nanofibrous scaffolds have great potential for bionanomaterial-cell transplantation therapy of neurodegenerative diseases and injuries of the nervous system. MSCs have been the highlight of many tissue engineering studies mainly because of their multipotential properties. We investigated the potential of human bone marrow derived Mesenchymal stem cells (MSCs) for neuronal differentiation in vitro on poly(L-lactic acid)-co-poly-(3-caprolactone)/Collagen (PLCL/Coll) nanofibrous scaffolds. PLCL and PLCL/Coll nanofibrous scaffolds were fabricated by electrospinning process and their chemical and mechanical characterizations were carried out using SEM, contact angle, FTIR, and tensile instrument. The differentiation of MSCs was carried out using neuronal inducing factors including beta-mercaptoethanol, epidermal growth factor, nerve growth factor and brain derived growth factor in DMEM/F12 media. The proliferations of MSCs evaluated by MTS assay showed that the cells grown on PLCL/Coll nanofibrous scaffolds were comparatively higher (80%) than those on PLCL. Scanning electron microscopy results showed that MSCs differentiated on PLCL/Coll nanofibrous scaffolds showed neuronal morphology, with multipolar elongations and expressed neurofilament and nestin protein by immuno-fluorescent microscopy. Our studies on the differentiation of MSCs to neuronal cells on nanofibrous scaffolds suggest their potential application towards nerve regeneration.
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Introducing collagen, gelatin and fibroin into PLCL significantly improves cell adhesion ability of PLCL nerve conduit and promotes migration adhesion and growth of nerve cells.
“By introducing collagen, gelatin and fibroin into PLCL, the cell adhesion ability of PLCL nerve conduit can be significantly improved, and the migration adhesion and growth of nerve cells can be promoted [183, 184].”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [184]
Preparation of PLCL into oriented or patterned spinning is beneficial to differentiation of neural stem cells and establishment of signal pathways between nerve cells.
“The preparation of PLCL into oriented or patterned spinning is beneficial to the differentiation of neural stem cells and the establishment of signal pathways between nerve cells [184].”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [184]
Cites
Multilineage potential of adult human mesenchymal stem cells
Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K., Douglas, R., Mosca, J. D., Moorman, M. A., Simonetti, D. W., Craig, S., & Marshak, D. R. (1999) · Science · doi:10.1126/science.284.5411.143 · Cited by 21,052 · see citing works
Human Mesenchymal Stem Cells Differentiate to a Cardiomyocyte Phenotype in the Adult Murine Heart
Toma, C., Pittenger, M. F., Cahill, K. S., Byrne, B. J., & Kessler, P. (2002) · Circulation · doi:10.1161/hc0102.101442 · Cited by 2,234 · see citing works
Biomimetic materials for tissue engineering
Shin, H., Jo, S., & Mikos, A. G. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00339-9 · Cited by 1,616 · see citing works
Adult Bone Marrow Stromal Cells Differentiate into Neural Cells in Vitro
Sanchez‐Ramos, J., Song, S., Cardozo‐Pelaez, F., Hazzi, C., Stedeford, T. J., Willing, A. E., Freeman, T. B., Saporta, S., Janssen, W. E., Patel, N. A., et al. (2000) · Experimental Neurology · doi:10.1006/exnr.2000.7389 · Cited by 1,562 · see citing works
Biomimetic materials for tissue engineering
Ma, P. X. (2007) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2007.08.041 · Cited by 1,310 · see citing works
Induction of Midbrain Dopaminergic Neurons from ES Cells by Stromal Cell–Derived Inducing Activity
Kawasaki, H., Mizuseki, K., Nishikawa, S., Kaneko, S., Kuwana, Y., Nakanishi, S., Nishikawa, S., & Sasai, Y. (2000) · Neuron · doi:10.1016/s0896-6273(00)00083-0 · Cited by 1,277 · see citing works
Electrospun poly(ɛ-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering
Ghasemi‐Mobarakeh, L., Prabhakaran, M. P., Morshed, M., Nasr‐Esfahani, M. H., & Ramakrishna, S. (2008) · Biomaterials · doi:10.1016/j.biomaterials.2008.08.007 · Cited by 1,153 · see citing works
Self-citationLi, W., Tuli, R., Okafor, C. C., Derfoul, A., Danielson, K. G., Hall, D. J., & Tuan, R. S. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2004.03.005 · Cited by 928 · see citing works
Cellular Adhesiveness and Extracellular Substrata
Grinnell, F. (1978) · International review of cytology · doi:10.1016/s0074-7696(08)62241-x · Cited by 825 · see citing works
Human bone marrow stromal cell responses on electrospun silk fibroin mats
Jin, H., Chen, J., Karageorgiou, V., Altman, G. H., & Kaplan, D. L. (2003) · Biomaterials · doi:10.1016/s0142-9612(03)00609-4 · Cited by 631 · see citing works
Barberi, T., Klivènyi, P., Calingasan, N. Y., Lee, H., Kawamata, H., Loonam, K., Perrier, A. L., Brusés, J. L., Rubio, M. E., Topf, N., et al. (2003) · Nature Biotechnology · doi:10.1038/nbt870 · Cited by 622 · see citing works
Li, W., Tuli, R., Huang, X., Laquerrière, P., & Tuan, R. S. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.01.002 · Cited by 615 · see citing works
Electrospinning of Nanofiber Fibrinogen Structures
Wnek, G. E., Carr, M. E., Simpson, D. G., et al. (2002) · Nano Letters · doi:10.1021/nl025866c · Cited by 555 · see citing works
Deng, W., Obrocka, M., Fischer, I., & Prockop, D. J. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.4570 · Cited by 485 · see citing works
Efficient generation of neural stem cell-like cells from adult human bone marrow stromal cells
Hermann, A., Gastl, R., Liebau, S., Popa, M. O., Fiedler, J., Boehm, B. O., Maisel, M., Lerche, H., Schwarz, J., Brenner, R. E., et al. (2004) · Journal of Cell Science · doi:10.1242/jcs.01307 · Cited by 449 · see citing works
Wislet‐Gendebien, S., Hans, G. A., Leprince, P., Rigo, J., Moonen, G., & Rogister, B. (2005) · Stem Cells · doi:10.1634/stemcells.2004-0149 · Cited by 427 · see citing works
Xin, X., Hussain, M. A., & Mao, J. J. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.08.042 · Cited by 378 · see citing works
Growth of Mesenchymal Stem Cells on Electrospun Type I Collagen Nanofibers
Shih, Y.-R. V., Chen, C., Tsai, S., Wang, Y. J., & Lee, O. K. (2006) · Stem Cells · doi:10.1634/stemcells.2006-0253 · Cited by 322 · see citing works
Mechanical properties of acellular peripheral nerve
Borschel, G. H., Kia, K. F., Kuzon, W. M., & Dennis, R. G. (2003) · Journal of Surgical Research · doi:10.1016/s0022-4804(03)00255-5 · Cited by 284 · see citing works
Krampera, M., Marconi, S., Pasini, A., Galiè, M., Rigotti, G., Mosna, F., Tinelli, M., Lovato, L., Anghileri, E., Andreini, A., et al. (2006) · Bone · doi:10.1016/j.bone.2006.09.006 · Cited by 241 · see citing works
Osteogenic differentiation of mesenchymal stem cells in self-assembled peptide-amphiphile nanofibers
Hosseinkhani, H., Hosseinkhani, M., Tian, F., Kobayashi, H., & Tabata, Y. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.03.030 · Cited by 225 · see citing works
Surface modified electrospun nanofibrous scaffolds for nerve tissue engineering
Prabhakaran, M. P., Venugopal, J. R., Chan, C. K., et al. (2008) · Nanotechnology · doi:10.1088/0957-4484/19/45/455102 · Cited by 221 · see citing works
Self-citationRathjen, J., Haines, B. P., Hudson, K. M., Nesci, A., Dunn, S., & Rathjen, P. D. (2002) · Development · doi:10.1242/dev.129.11.2649 · Cited by 162 · see citing works
Mareschi, K., Novara, M., Rustichelli, D., Ferrero, I., Guido, D., Carbone, E., Médico, E., Madon, E., Vercelli, A., & Fagioli, F. (2006) · Experimental Hematology · doi:10.1016/j.exphem.2006.06.020 · Cited by 153 · see citing works
Ye, W. P., Du, F. S., Jin, W. H., Yang, J. Y., & Xu, Y. (1997) · Reactive and Functional Polymers · doi:10.1016/s1381-5148(96)00081-8 · Cited by 142 · see citing works
Electrospun‐modified nanofibrous scaffolds for the mineralization of osteoblast cells
Venugopal, J. R., Low, S., Choon, A. T., Kumar, A., & Ramakrishna, S. (2007) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.31538 · Cited by 140 · see citing works
Self-citationChen, Y., Teng, F. Y. H., & Tang, B. L. (2006) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-006-6019-5 · Cited by 98 · see citing works
Kobune, M., Kawano, Y., Ito, Y., et al. (2003) · Experimental Hematology · doi:10.1016/s0301-472x(03)00177-2 · Cited by 95 · see citing works
Hosseinkhani, H., Hosseinkhani, M., & Kobayashi, H. (2006) · Biomedical Materials · doi:10.1088/1748-6041/1/1/002 · Cited by 86 · see citing works
Design of Resorbable Porous Tubular Copolyester Scaffolds for Use in Nerve Regeneration
Plikk, P., Målberg, S., & Albertsson, A. (2009) · Biomacromolecules · doi:10.1021/bm900093r · Cited by 86 · see citing works
Generating neuron-like cells from BM-derived mesenchymal stromal cells in vitro
Choong, P., Mok, P. L., Cheong, S., Leong, C. F., & Then, K.-Y. (2007) · Cytotherapy · doi:10.1080/14653240701196829 · Cited by 77 · see citing works
Proteasome inhibition results in increased poly-ADP-ribosylation: Implications for neuron death
Keller, J. N., & Markesbery, W. R. (2000) · Journal of Neuroscience Research · doi:10.1002/1097-4547(20000815)61:4 · Cited by 54 · see citing works
Wenisch, S., Trinkaus, K., Hild, A., et al. (2006) · Bone · doi:10.1016/j.bone.2005.10.021 · Cited by 53 · see citing works
Ishii, K., Katayama, M., Hori, K., et al. (1993) · Neuroscience Letters · doi:10.1016/0304-3940(93)90371-q · Cited by 49 · see citing works
Lemmouchi, Y., & Schacht, E. H. (1997) · Journal of Controlled Release · doi:10.1016/s0168-3659(96)01569-6 · Cited by 43 · see citing works
Schofer, M. D., Boudriot, U., Wack, C., et al. (2008) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-008-3634-8 · Cited by 43 · see citing works
Zhou, J., Chu, J., & Chen, X. (2007) · Cell Biology International · doi:10.1016/j.cellbi.2007.08.015 · Cited by 21 · see citing works
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Neural tissue engineering options for peripheral nerve regeneration
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Zhao, Y., & Liu, H. (2026) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-026-07016-3 · Cited by 7 · see citing works
- Authors
- Prabhakaran, M. P., Venugopal, J. R., & Ramakrishna, S.
- Affiliations
- National University of Singapore (SG)
- Corresponding author
- Prabhakaran, Molamma P.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2009-10
- Volume
- 30
- Issue
- 28
- Pages
- 4996-5003
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2075477218
- Cited by
- 311
- References
- 37
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 6 · 2023: 12 · 2022: 11 · 2021: 14 · 2020: 17 · 2019: 22 · 2018: 24 · 2017: 22 · 2016: 22 · 2015: 31 · 2014: 26 · 2013: 23 · 2012: 31
- FWCI
- 10.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- National University of Singapore (submittedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Keywords
- mesenchymal stem cells, neuronal differentiation, bone marrow-derived stem cells, electrospun nanofibrous scaffolds, poly(L-lactic acid), collagen, neural tissue engineering, nerve regeneration, neurotrophic factors, cell proliferation, neurofilaments, nestin, neurodegenerative diseases
- MeSH terms
- Cells, Cultured, Humans, Nerve Tissue, Neurons, Cell Culture Techniques, Tissue Engineering, Cell Proliferation, Nanostructures, Tissue Scaffolds, Neurogenesis, Mesenchymal Stem Cells
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