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Serum heat inactivation affects protein corona composition and nanoparticle uptake
Abstract
Nanoparticles are of an appropriate size to interact with cells, and are likely to use a range of cellular machinery for internalisation and trafficking to various sub-cellular compartments. It is now understood that once in contact with biological fluids, the nanoparticle surface gets covered by a highly specific layer of proteins, forming the nanoparticle protein corona. This protein layer is stable for times longer than the typical time scale of nanoparticle import, and thus can impact on particle uptake and trafficking inside the cells. In this work, the effect of the corona composition on nanoparticle uptake has been investigated, by studying the impact of serum heat inactivation and complement depletion on the load of nanoparticles accumulated inside the cell. For the same material and nanoparticle size, cellular uptake was found to be significantly different when the nanoparticles were dispersed in medium where the serum was heat inactivated or not heat inactivated, even for non-specialized cells, suggesting that different sera can lead to different nanoparticle doses. The fact that uptake was correlated with the amount of protein bound into the nanoparticle corona suggests the need for commonly agreed dispersion protocols for in vitro nanoparticle-cell studies.
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“Indeed, due to interactions with their surroundings, the dispersion media play an important role in defining the NPs dose, but also in determining the NPs “biological” identity, which is the set of proteins and other biomolecules associated with the NP and specifically the outer surface of the absorbed corona which forms the interface to engage with biological receptors [113–116].”
From Particle toxicology and health - where are we? (2019) · cites this paper as [114]
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Cites
Time Evolution of the Nanoparticle Protein Corona
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Sorted by citations
Monopoli, M. P., Walczyk, D., Campbell, A., Elia, G., Lynch, I., Baldelli Bombelli, F., & Dawson, K. A. (2011) · Journal of the American Chemical Society · doi:10.1021/ja107583h · Cited by 1,835
Self-citationWalkey, C. D., & Chan, W. C. W. (2012) · Chem. Soc. Rev. · doi:10.1039/c1cs15233e · Cited by 1,758
Particle toxicology and health - where are we?
Riediker, M., Zink, D., Kreyling, W., Oberdörster, G., Elder, A., Graham, U., Lynch, I., Duschl, A., Ichihara, G., Ichihara, S., et al. (2019) · Particle and Fibre Toxicology · doi:10.1186/s12989-019-0302-8 · Cited by 237
Self-citationHo, Y. T., Kamm, R. D., & Kah, J. C. Y. (2018) · Nanoscale · doi:10.1039/c8nr02393j · Cited by 102
Derakhshankhah, H., Sajadimajd, S., Jafari, S., Izadi, Z., Sarvari, S., Sharifi, M., Falahati, M., Moakedi, F., Muganda, W. C. A., Müller, M., et al. (2020) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2020.102149 · Cited by 48
Metadata
- Authors
- Lesniak, A., Campbell, A., Monopoli, M. P., Lynch, I., Salvati, A., & Dawson, K. A.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2010-12
- Volume
- 31
- Issue
- 36
- Pages
- 9511-9518
- ISSN
- 0142-9612
- Cited by
- 266
- References
- 1
- Language
- en
- Funders
- Irish Government’s Programme for Research
- SIGHT (IST-2005-033700)
- EU FP6 NanoInteract (NMP4-CT-2006-033231)
- Irish Research Council for Science, Engineering and Technology
- Science Foundation Ireland (SFI/SRC/B1155)
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