Time Evolution of the Nanoparticle Protein Corona
Abstract
In this work, we explore the formation of the protein corona after exposure of metallic Au nanoparticles (NPs), with sizes ranging from 4 to 40 nm, to cell culture media containing 10% of fetal bovine serum. Under in vitro cell culture conditions, zeta potential measurements, UV-vis spectroscopy, dynamic light scattering and transmission electron microscope analysis were used to monitor the time evolution of the inorganic NP-protein corona formation and to characterize the stability of the NPs and their surface state at every stage of the experiment. As expected, the red-shift of the surface plasmon resonance peak, as well as the drop of surface charge and the increase of the hydrodynamic diameter indicated the conjugation of proteins to NPs. Remarkably, an evolution from a loosely attached toward an irreversible attached protein corona over time was observed. Mass spectrometry of the digested protein corona revealed albumin as the most abundant component which suggests an improved biocompatibility.
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Exchangeable apolipoproteins (such as ApoA, C, and E) are found in protein coronas formed on the surface of a variety of drug delivery systems.
“Exchangeable apolipoproteins (such as ApoA, C, and E) that can direct the transport of lipids through lymphatic and circulatory systems have been found in protein coronas formed on the surface of a variety of drug delivery systems15–17.”
From Brain-targeted drug delivery by manipulating protein corona functions (2019) · cites this paper as [16]
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Block copolymer micelles in nanomedicine applications
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Brain-targeted drug delivery by manipulating protein corona functions
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Self-citation
- Authors
- Casals, E., Pfaller, T., Duschl, A., Oostingh, G. J., & Puntes, V.
- Affiliations
- Institut Català de Nanociència i Nanotecnologia (ES)
- University of Salzburg (AT)
- Institut Català de Nanotecnologia, Bellaterra, Barcelona, Spain
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-06-16
- Volume
- 4
- Issue
- 7
- Pages
- 3623-3632
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W1997153101
- Cited by
- 1,211
- References
- 1
- Citations by year
- 2026: 50 · 2025: 56 · 2024: 38 · 2023: 65 · 2022: 79 · 2021: 83 · 2020: 87 · 2019: 105 · 2018: 85 · 2017: 79 · 2016: 101 · 2015: 87 · 2014: 105 · 2013: 79 · 2012: 71
- FWCI
- 25.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Nano (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
- Keywords
- protein corona, gold nanoparticles, time evolution, fetal bovine serum, zeta potential, hydrodynamic diameter, albumin, nanoparticle stability, biocompatibility
- MeSH terms
- Animals, Cattle, Citrates, Fatty Acids, Gold, Particle Size, Proteins, Serum Albumin, Bovine, Sulfhydryl Compounds, Time Factors, Metal Nanoparticles, Static Electricity
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