Cited by 265Bone Tissue Engineering MaterialsSilk-based biomaterials and applications3D Printing in Biomedical Research

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Nucleation and growth of mineralized bone matrix on silk-hydroxyapatite composite scaffolds

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Bhumiratana, Sarindr, Grayson, Warren L., Castaneda, Andrea, Rockwood, Danielle N., Gil, Eun S., Kaplan, David L., Vunjak-Novakovic, Gordana·2011·Biomaterials

DOI 10.1016/j.biomaterials.2010.12.058PMC PMC3042545

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Abstract

We describe a composite hydroxyapatite (HA)-silk fibroin scaffold designed to induce and support the formation of mineralized bone matrix by human mesenchymal stem cells (hMSCs) in the absence of osteogenic growth factors. Porous three-dimensional silk scaffolds were extensively used in our previous work for bone tissue engineering and showed excellent biodegradability and biocompatibility. However, silk is not an osteogenic material and has a compressive stiffness significantly lower than that of native bone. In the present study, we explored the incorporation of silk sponge matrices with HA (bone mineral) micro-particles to generate highly osteogenic composite scaffolds capable of inducing the in vitro formation of tissue-engineered bone. Different amounts of HA were embedded in silk sponges at volume fractions of 0%, 1.6%, 3.1% and 4.6% to enhance the osteoconductive activity and mechanical properties of the scaffolds. The cultivation of hMSCs in the silk/HA composite scaffolds under perfusion conditions resulted in the formation of bone-like structures and an increase in the equilibrium Young's modulus (up to 4-fold or 8-fold over 5 or 10 weeks of cultivation, respectively) in a manner that correlated with the initial HA content. The enhancement in mechanical properties was associated with the development of the structural connectivity of engineered bone matrix. Collectively, the data suggest two mechanisms by which the incorporated HA enhanced the formation of tissue engineered bone: through osteoconductivity of the material leading to increased bone matrix production, and by providing nucleation sites for new mineral resulting in the connectivity of trabecular-like architecture.

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Authors: Bhumiratana, S., Grayson, W. L., Castaneda, A., Rockwood, D. N., Gil, E. S., Kaplan, D. L., & Vunjak-Novakovic, G.
Affiliations: Columbia University (US)
Johns Hopkins University (US)
Tufts University (US)
ORCID: Bhumiratana, Sarindr
Grayson, Warren L.
Kaplan, David L.
Venue: Biomaterials
Publisher: Elsevier BV
Published: 2011-04
Volume: 32
Issue: 11
Pages: 2812-2820
ISSN: 0142-9612 (ISSN-L)
DOI: 10.1016/j.biomaterials.2010.12.058
PMC: PMC3042545
OpenAlex: W2020617826
Cited by: 265
References: 41
Citations by year: 2026: 2 · 2025: 9 · 2024: 15 · 2023: 9 · 2022: 12 · 2021: 9 · 2020: 15 · 2019: 16 · 2018: 18 · 2017: 23 · 2016: 30 · 2015: 25 · 2014: 24 · 2013: 32 · 2012: 18
FWCI: 15.51
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Biomaterials (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
Funders: National Institutes of Health
National Institute of Biomedical Imaging and Bioengineering (EB 003210, R01EB003210, P41EB002520)
National Institute of Dental and Craniofacial Research (DE-016525, R01DE016525)
Topics: Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords: silk fibroin, hydroxyapatite, composite scaffolds, bone tissue engineering, human mesenchymal stem cells, osteoconductivity, mineral nucleation, scaffold mechanical properties, Young's modulus, trabecular bone microarchitecture, perfusion bioreactor
MeSH terms: Calcification, Physiologic, Cell Survival, Cells, Cultured, Humans, Immunohistochemistry, Microscopy, Electron, Scanning, Durapatite, Tissue Engineering, Silk, Tissue Scaffolds, Mesenchymal Stem Cells

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Nucleation and growth of mineralized bone matrix on silk-hydroxyapatite composite scaffolds

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