A biomimetic multilayer nanofiber fabric fabricated by electrospinning and textile technology from polylactic acid and Tussah silk fibroin as a scaffold for bone tissue engineering
Abstract
To engineer bone tissue, a scaffold with good biological properties should be provided to approximate the hierarchical structure of collagen fibrils in natural bone. In this study, we fabricated a novel scaffold consisting of multilayer nanofiber fabrics (MLNFFs) by weaving nanofiber yarns of polylactic acid (PLA) and Tussah silk fibroin (TSF). The yarns were fabricated by electrospinning, and we found that spinnability, as well as the mechanical properties of the resulting scaffold, was determined by the ratio between polylactic acid and Tussah silk fibroin. In particular, a 9:1 mixture can be spun continuously into nanofiber yarns with narrow diameter distribution and good mechanical properties. Accordingly, woven scaffolds based on this mixture had excellent mechanical properties, with Young's modulus 417.65MPa and tensile strength 180.36MPa. For nonwoven scaffolds fabricated from the same materials, the Young's modulus and tensile strength were 2- and 4-fold lower, respectively. Woven scaffolds also supported adhesion and proliferation of mouse mesenchymal stem cells, and promoted biomineralization via alkaline phosphatase and mineral deposition. Finally, the scaffolds significantly enhanced the formation of new bone in damaged femoral condyle in rabbits. Thus, the scaffolds are potentially suitable for bone tissue engineering because of biomimetic architecture, excellent mechanical properties, and good biocompatibility.
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PLA has good biocompatibility, biodegradability, gloss, transparency, and antibacterial, flame-retardant, and oil- and water-resistant properties.
“Simultaneously, polylactic acid material has good biocompatibility, biodegradability, gloss, and transparency, and antibacterial, flame-retardant, and oil- and water-resistant properties.”
From Synthesis and Biological Application of Polylactic Acid (2020) · cites this paper as [18]
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Synthesis and Biological Application of Polylactic Acid
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- Authors
- Shao, W., He, J., Han, Q., Sang, F., Wang, Q., Chen, L., Cui, S., & Ding, B.
- Affiliations
- Zhongyuan University of Technology (CN)
- First Affiliated Hospital of Henan University of Traditional Chinese Medicine (CN)
- Tiangong University (CN)
- Corresponding author
- He, Jianxin
- Ding, Bin
- Venue
- Materials Science and Engineering: C
- Publisher
- Elsevier BV
- Published
- 2016-10
- Volume
- 67
- Pages
- 599-610
- ISSN
- 0928-4931 (ISSN-L)
- OpenAlex
- W2402871203
- Cited by
- 114
- References
- 46
- Citations by year
- 2026: 7 · 2025: 13 · 2024: 18 · 2023: 9 · 2022: 10 · 2021: 9 · 2020: 18 · 2019: 8 · 2018: 8 · 2017: 12 · 2016: 2
- FWCI
- 4.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Materials Science and Engineering C (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (51203196, U1204510)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Keywords
- bone tissue engineering, polylactic acid, mechanical properties, biomineralization
- MeSH terms
- Animals, Calcification, Physiologic, Cells, Cultured, Femur, Fibroins, Polyesters, Rabbits, Bone Substitutes, Tissue Engineering, Mice, Tissue Scaffolds, Elastic Modulus, Nanofibers, Mesenchymal Stem Cells
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