The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
The performance of human mesenchymal stem cells encapsulated in cell-degradable polymer-peptide hydrogels
Open accessAbstract
Thiol-ene photopolymerization offers a unique platform for the formation of peptide-functionalized poly(ethylene glycol) hydrogels and the encapsulation, culture and differentiation of cells. Specifically, this photoinitiated polymerization scheme occurs at neutral pH and can be controlled both spatially and temporally. Here, we have encapsulated human mesenchymal stem cells (hMSCs) in matrix metalloproteinase (MMP) degradable and cell-adhesive hydrogels using thiol-ene photopolymerization. We find that hMSCs survive equally well in this system, regardless of MMP-degradability. When hMSCs are encapsulated in these cell-degradable hydrogels, they survive and are able to proliferate. In classic hMSC differentiation medias, hMSCs locally remodel their microenvironment and take on characteristic morphologies; hMSCs cultured in growth or osteogenic differentiation media are less round, as measured by elliptical form factor, and are smaller than hMSCs cultured in chondrogenic or adipogenic differentiation media. In addition, hMSCs encapsulated in completely cell-degradable hydrogels and cultured in osteogenic, chondrogenic, or adipogenic differentiation media generally express increased levels of specific differentiation markers as compared to cells in hydrogels that are not cell-degradable. These studies demonstrate the ability to culture and differentiate hMSCs in MMP-degradable hydrogels polymerized via a thiol-ene reaction scheme and that increased cell-mediated hydrogel degradability facilitates directed differentiation of hMSCs.
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Cites
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Self-citationSubstrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture
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Designing degradable hydrogels for orthogonal control of cell microenvironments
Kharkar, P. M., Kiick, K. L., & Kloxin, A. M. (2013) · Chem. Soc. Rev. · doi:10.1039/c3cs60040h · Cited by 717
Sulfur and Its Role In Modern Materials Science
Boyd, D. A. (2016) · Angewandte Chemie International Edition · doi:10.1002/anie.201604615 · Cited by 512
Analyte-Responsive Hydrogels: Intelligent Materials for Biosensing and Drug Delivery
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Metadata
- Authors
- Anderson, S. B., Lin, C.-C., Kuntzler, D. V., & Anseth, K. S.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2011-05
- Volume
- 32
- Issue
- 14
- Pages
- 3564-3574
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W1964681850
- Cited by
- 352
- References
- 39
- FWCI
- 14.20
- Type
- journal article
- Language
- en
- Open access
- green
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