Cited by 679Hydrogels: synthesis, properties, applicationsAdvanced Polymer Synthesis and CharacterizationSupramolecular Self-Assembly in Materials

Synthesis and Physicochemical Characterization of End-Linked Poly(ethylene glycol)-co-peptide Hydrogels Formed by Michael-Type Addition

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Lutolf, M. P., Hubbell, J. A.·2003·Biomacromolecules

Abstract

The synthesis of novel hybrid hydrogels by stepwise copolymerization of multiarm vinyl sulfone-terminated poly(ethylene glycol) macromers and alpha-omega cysteine oligopeptides via Michael-type additions is described. Cross-linking kinetics, studied by in situ rheometry, can be controlled by pH and the presence of charged amino acid residues in close proximity to the Cys, which modulates the pK(a) of the thiol group. These end-linked networks were characterized by their equilibrium swelling in water, by their viscoelastic properties in the swollen state, and by their soluble fraction. It was demonstrated that structure and properties are very sensitive to the preparation state including stoichiometry and precursor concentration and less sensitive to the pH during cross-linking. For each network the concentration of elastically active chains (nu) was calculated from experimentally determined sol fractions using Miller-Macosko theory and compared to values obtained from swelling and rheometry studies and by calculation from Flory's classical network models. Hydrogels were also prepared with varying macromer structures, and their properties were shown to respond to both macromer functionality and molecular weight.

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Crossref · 10.1021/bm025744e

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  • Synthetic hydrogels can be functionalized with biologically active moieties that permit the growth and spread of cells; concentration and spacing of these cues can be changed independently.

    “Synthetic hydrogels can be functionalized with biologically active moieties that permit the growth and spread of cells146–151; concentration and spacing of these cues can be changed independently152–155.”

    From Towards organoid culture without Matrigel (2021) · cites this paper as [150]

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Authors
Lutolf, M. P., & Hubbell, J. A.
Affiliations
  • Department of Materials and Institute for Biomedical Engineering, ETH Zurich and the University of Zurich, Zurich, Switzerland
  • University of Zurich (CH)
Corresponding author
Hubbell, J. A.
Venue
Biomacromolecules
Published
2003-02-26
Volume
4
Issue
3
Pages
713-722
ISSN
  • 1525-7797 (ISSN-L)
  • 1526-4602
OpenAlex
W2082182581
Cited by
679
References
26
Citations by year
2026: 10 · 2025: 12 · 2024: 13 · 2023: 21 · 2022: 13 · 2021: 28 · 2020: 36 · 2019: 30 · 2018: 27 · 2017: 42 · 2016: 39 · 2015: 33 · 2014: 43 · 2013: 44 · 2012: 47
FWCI
6.29
Type
journal article
Language
en
Open access
Locations
  • Biomacromolecules (publishedVersion)
  • PubMed (publishedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Physical Sciences › Chemistry › Organic Chemistry › Advanced Polymer Synthesis and Characterization
  • Physical Sciences › Materials Science › Biomaterials › Supramolecular Self-Assembly in Materials
Keywords
Michael-type addition, thiol pKa, swelling behavior, viscoelasticity, sol fraction, molecular weight effects, hybrid hydrogels
MeSH terms
Chemistry, Physical, Oligopeptides, Polyethylene Glycols, DNA Adducts, Hydrogels, Chemical Phenomena

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