Hydrogels as extracellular matrix mimics for 3D cell culture

Tibbitt, Mark W.; Anseth, Kristi S.

doi:10.1002/bit.22361

Cited by 2,727Hydrogels: synthesis, properties, applications3D Printing in Biomedical ResearchCellular Mechanics and Interactions

Hydrogels as extracellular matrix mimics for 3D cell culture

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Tibbitt, Mark W., Anseth, Kristi S.·2009·Biotechnology and Bioengineering

DOI 10.1002/bit.22361PMC PMC2997742

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Abstract

Methods for culturing mammalian cells ex vivo are increasingly needed to study cell and tissue physiology and to grow replacement tissue for regenerative medicine. Two‐dimensional culture has been the paradigm for typical in vitro cell culture; however, it has been demonstrated that cells behave more natively when cultured in three‐dimensional environments. Permissive, synthetic hydrogels and promoting, natural hydrogels have become popular as three‐dimensional cell culture platforms; yet, both of these systems possess limitations. In this perspective, we discuss the use of both synthetic and natural hydrogels as scaffolds for three‐dimensional cell culture as well as synthetic hydrogels that incorporate sophisticated biochemical and mechanical cues as mimics of the native extracellular matrix. Ultimately, advances in synthetic–biologic hydrogel hybrids are needed to provide robust platforms for investigating cell physiology and fabricating tissue outside of the organism. Biotechnol. Bioeng. 2009;103: 655–663. © 2009 Wiley Periodicals, Inc.

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PEG-based hydrogels can be functionalized with biological moieties in a controlled and systematic manner to create environments specific to a tissue engineering application.
“They present a ‘blank slate,’ which can be functionalized with biological moieties in a controlled and systematic manner creating environments that are specific for the tissue engineering application.1”
From Student award winner in the undergraduate category for the society of biomaterials 9th World Biomaterials Congress, Chengdu, China, June 1–5, 2012: The effects of substrate stiffness on the in vitro activation of macrophages and in vivo host response to poly(ethylene glycol)‐based hydrogels (2012) · cites this paper as [1]
Many tissue engineering strategies using PEG hydrogels incorporate proteins or peptides to support cell adhesion and promote cell-material interactions.
“Many tissue engineering strategies that use PEG hydrogels incorporate proteins or peptides to support cell adhesion and promote cell-material interactions.1”
From Student award winner in the undergraduate category for the society of biomaterials 9th World Biomaterials Congress, Chengdu, China, June 1–5, 2012: The effects of substrate stiffness on the in vitro activation of macrophages and in vivo host response to poly(ethylene glycol)‐based hydrogels (2012) · cites this paper as [1]

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Authors: Tibbitt, M. W., & Anseth, K. S.
Affiliations: University of Colorado Boulder (US)
Howard Hughes Medical Institute (US)
Corresponding author: Anseth, Kristi S.
ORCID: Tibbitt, Mark W.
Anseth, Kristi S.
Venue: Biotechnology and Bioengineering
Publisher: Wiley
Published: 2009-05-26
Volume: 103
Issue: 4
Pages: 655-663
ISSN: 0006-3592 (ISSN-L)
1097-0290
DOI: 10.1002/bit.22361
PMC: PMC2997742
OpenAlex: W2021550972
Cited by: 2,727
References: 69
Citations by year: 2026: 132 · 2025: 141 · 2024: 153 · 2023: 185 · 2022: 202 · 2021: 238 · 2020: 229 · 2019: 222 · 2018: 246 · 2017: 195 · 2016: 187 · 2015: 132 · 2014: 126 · 2013: 106 · 2012: 92
FWCI: 3.76
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Biotechnology and Bioengineering (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: Howard Hughes Medical Institute
National Institute of General Medical Sciences (T32GM065103)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords: hydrogels, extracellular matrix mimics, 3D cell culture, synthetic hydrogels, natural hydrogels, tissue engineering scaffolds, mechanical cues, biochemical cues, regenerative medicine, ex vivo cell culture
MeSH terms: Animals, Cells, Cultured, Extracellular Matrix, Humans, Mammals, Hydrogel, Polyethylene Glycol Dimethacrylate, Tissue Engineering

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Hydrogels as extracellular matrix mimics for 3D cell culture

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