The DOI matches its Crossref record; 57 of the 59 references it cites were found in a registry or an index.
Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system
Abstract
End-stage renal failure is a devastating disease, with donor organ transplantation as the only functional restorative treatment. The current number of donor organs meets less than one-fifth of demand, so regenerative medicine approaches have been proposed as potential therapeutic alternatives. One such approach for whole large-organ bioengineering is to combine functional renal cells with a decellularized porcine kidney scaffold. The efficacy of cellular removal and biocompatibility of the preserved porcine matrices, as well as scaffold reproducibility, are critical to the success of this approach. We evaluated the effectiveness of 0.25 and 0.5% sodium dodecyl sulfate (SDS) and 1% Triton X-100 in the decellularization of adult porcine kidneys. To perform the decellularization, a high-throughput system was designed and constructed. In this study all three methods examined showed significant cellular removal, but 0.5% SDS was the most effective detergent (<50 ng DNA/mg dry tissue). Decellularized organs retained intact microarchitecture including the renal vasculature and essential extracellular matrix components. The SDS-treated decellularized scaffolds were non-cytotoxic to primary human renal cells. This method ensures clearance of porcine cellular material (which directly impacts immunoreactivity during transplantation) and preserves the extracellular matrix and cellular compatibility of these renal scaffolds. Thus, we have developed a rapid decellularization method that can be scaled up for use in other large organs, and this represents a step toward development of a transplantable organ using tissue engineering techniques.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Although chemical agents play a key role in the decellularization and removal of the immunogenic materials from the decellularized tissues, they may cause ECM damage and affect the biocompatibility of the yielded decellularized tissues [5,7,[90], [91], [92]].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [90]
Citations
Cites
1 retracted
RETRACTED: Clinical transplantation of a tissue-engineered airway
Macchiarini, P., Jungebluth, P., Go, T., Asnaghi, M. A., Rees, L., Cogan, T. A., Dodson, A., Martorell, J., Bellini, S., Parnigotto, P. P., et al. (2008) · The Lancet · doi:10.1016/s0140-6736(08)61598-6 · Cited by 1,431
RetractedTheory and Practice of Histological Techniques
Bancroft, J. D., & Gamble, M. (1990) · Cited by 6,948
Wolfe, R. A., Ashby, V. B., Milford, E. L., OJO, A. O., Ettenger, R. E., Agodoa, L. Y. C., Held, P. J., & Port, F. K. (1999) · New England Journal of Medicine · doi:10.1056/nejm199912023412303 · Cited by 5,370
Prevalence of Chronic Kidney Disease in the United States
Coresh, J., Selvin, E., Stevens, L. A., Manzi, J., Kusek, J. W., Eggers, P., Van Lente, F., & Levey, A. S. (2007) · JAMA · doi:10.1001/jama.298.17.2038 · Cited by 4,895
An overview of tissue and whole organ decellularization processes
Crapo, P. M., Gilbert, T. W., & Badylak, S. F. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.01.057 · Cited by 3,591
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Ott, H. C., Matthiesen, T. S., Goh, S.-K., Black, L. D., Kren, S. M., Netoff, T. I., & Taylor, D. A. (2008) · Nature Medicine · doi:10.1038/nm1684 · Cited by 2,650
Decellularization of tissues and organs
Gilbert, T. W., Sellaro, T. L., & Badylak, S. F. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.02.014 · Cited by 2,222
Tissue-engineered autologous bladders for patients needing cystoplasty
Atala, A., Bauer, S. B., Söker, S., Yoo, J. J., & Retik, A. B. (2006) · The Lancet · doi:10.1016/s0140-6736(06)68438-9 · Cited by 1,857
Self-citationExtracellular matrix as a biological scaffold material: Structure and function
BADYLAK, S., FREYTES, D., & GILBERT, T. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2008.09.013 · Cited by 1,671
Uygun, B. E., Soto-Gutierrez, A., Yagi, H., Izamis, M.-L., Guzzardi, M. A., Shulman, C., Milwid, J., Kobayashi, N., Tilles, A., Berthiaume, F., et al. (2010) · Nature Medicine · doi:10.1038/nm.2170 · Cited by 1,370
Flagged first, then by citations
2 of the 59 references couldn’t be matched to a known paper.
Research works printed but not linked: 1
- Baptista, 2007, Liver tissue engineering using an acellular whole organ bioscaffold with perfusion cell seeding, Tissue Eng, 13, 1721
Cited by
Sorted by citations
Items 301–363 of 363
Off-the-Shelf Tissue-Engineered Vascular Conduits: Clinical Translation
Fioretta, E. S., von Boehmer, L., Generali, M., Hoerstrup, S. P., & Emmert, M. Y. (2020) · Springer eBooks · doi:10.1007/978-3-030-05336-9_21 · Cited by 2
Decellularization Techniques for Tissue Engineering
Chen, G., Kawazoe, N., & Lu, H. (2015) · Encyclopedia of Analytical Chemistry · doi:10.1002/9780470027318.a9472 · Cited by 2
Naturally Derived Biomaterials: An Overview
Naveen Kumar, C. P., Kumar, V., Shrivastava, S., Gangwar, A. K., Gopinathan, A., Maiti, S. K., Saxena, S., Palakkara, S., Raja, R., & Raghuvanshi, P. D. S. (2022) · Springer protocols handbooks/Springer protocols · doi:10.1007/978-1-0716-2425-8_1 · Cited by 1
de Jong, J. M., Werkman, I. L., Kats, K., Bosma, J., Kooistra, S. M., Eggen, B. J. L., & Baron, W. (2026) · Heliyon · doi:10.1016/j.heliyon.2026.e44653 · Cited by 1
Tissue Bioengineering in Transplantation
Katari, R., Edgar, L., Enck, K., Peloso, A., Tamburrini, R., & Orlando, G. (2017) · Springer eBooks · doi:10.1007/978-3-319-62142-5_8 · Cited by 2
Whole-Organ Bioengineering—Current Tales of Modern Alchemy
Moran, E. C., Dhal, A., Vyas, D., Lanas, A., Söker, S., & Baptista, P. M. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-800548-4.00003-6 · Cited by 1
Self-citationEngineering of tissues and organs
Lee, S. J., & Atala, A. (2014) · Elsevier eBooks · doi:10.1533/9780857097163.3.347 · Cited by 2
Self-citationProduction of Decellularized Tissue-Derived Materials
Ahearne, M. (2024) · Springer eBooks · doi:10.1007/978-3-031-56363-8_41 · Cited by 1
Whole-Heart Tissue Engineering: Use of Three-Dimensional Matrix Scaffolds
Aubin, H., Hülsmann, J., Pinto, A. V., Lichtenberg, A., & Akhyari, P. (2014) · Springer eBooks · doi:10.1007/978-3-319-10972-5_7 · Cited by 1
Biological scaffolds for regenerative medicine
Agrawal, V., & Badylak, S. F. (2014) · Cambridge University Press eBooks · doi:10.1017/cbo9780511997839.012 · Cited by 1
Biomaterial-Assisted Stem Cell Engineering for Tissue Construction and Regeneration
Yan, X., Wang, B., Qi, C., Lowrey, J. J., & Du, Y. (2015) · Translational medicine research · doi:10.1007/978-94-017-7273-0_11 · Cited by 1
Renal Regeneration: The Bioengineering Approach
Salvatori, M., Peloso, A., Patel, T. H., Hemal, S., Zambon, J. P., Katari, R., & Orlando, G. (2013) · Elsevier eBooks · doi:10.1016/b978-0-12-398523-1.00017-3 · Cited by 1
Masereeuw, R., Vriend, J., & Wilmer, M. J. G. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-801734-0.00082-5 · Cited by 1
Santeramo, I., Wilm, B., & Murray, P. A. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-801734-0.00076-x · Cited by 1
Detecting Changes to the Extracellular Matrix in Liver Diseases
Dolin, C. E., Sato, T., Merchant, M. L., & Arteel, G. E. (2020) · Biology of extracellular matrix · doi:10.1007/978-3-030-58330-9_3 · Cited by 1
Modeli in vitro endokrine trebušne slinavke
Milojević, M., Stožer, A., & Maver, U. (2021) · Slovenian Medical Journal · doi:10.6016/zdravvestn.3001 · Cited by 1
Where Are We Going? Future Trends and Challenges
Lee, S. J., & Atala, A. (2014) · Wiley eBooks · doi:10.1002/9781118356692.ch11 · Cited by 1
Self-citationDecellularization of fish skin for tissue engineering applications
Lau, C. S. (2019) · doi:10.32657/10356/100060 · Cited by 1
Chen, G., & Kawazoe, N. (2019) · Encyclopedia of Analytical Chemistry · doi:10.1002/9780470027318.a9472.pub2 · Cited by 1
Keshvari, M. A., Afshar, A., Daneshi, S., Khoradmehr, A., Baghban, M., Miri, M. R., Azari, H., Behrouzi, P., Nabipour, I., Mahmudpour, M., et al. (2020) · Preprints.org · doi:10.20944/preprints202012.0027.v1 · Cited by 1
Moaness, M., & Mabrouk, M. (2024) · Wiley eBooks · doi:10.1002/9781119894407.ch3 · Cited by 1
Goh, S. K. (2015) · D-Scholarship@Pitt (University of Pittsburgh)
Developing a Scaffold from Porcine Adipose Tissue
Roehm, K. D. (2014) · SHAREOK (University of Oklahoma; Oklahoma State University; Central Oklahoma University)
Mitchell, M. G. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-805369-0.00004-3
Tissue Engineering of Renal Tissue (Kidney)
Rodrigues‐Díez, R., Benedetti, V., Remuzzi, G., & Xinaris, C. (2017) · Wiley eBooks · doi:10.1002/9783527689934.ch18
Controlled Differentiation of Human and Mouse Embryonic Stem Cells to Kidney Precursors
Sambi, M. (2013) · TSpace (University of Toronto)
Endothelialization of the Coronary Vasculature in Decellularized Heart Scaffolds
Canavan, J. L. (2016) · TigerPrints (Clemson University)
Regenerative Medicine Approaches for the Kidney
Ko, I. K., Yoo, J. J., & Atala, A. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-809880-6.00066-7
Self-citationKuna, V. K. (2018) · Gothenburg University Publications Electronic Archive (Gothenburg University)
Zambon, J. P., Chandra, P. K., Atala, A., & Yoo, J. J. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-410396-2.00033-5
Self-citationRecellularization of Decellularized Whole Organ Scaffolds: Elements, Progresses, and Challenges
Hu, J.-G., Huang, Y., Tan, J., Da, L., & Xie, H. (2021) · Springer eBooks · doi:10.1007/978-981-33-6962-7_6
Decellularized liver extracellular matrix for iPSC-based liver engineering
Acun, A., & Uygun, B. E. (2021) · Elsevier eBooks · doi:10.1016/b978-0-323-85766-6.00006-1
Matrix scaffolds in kidney engineering
Muir, S. M., Pointe, C. L., Marino, D. I., Asthana, A., & Orlando, G. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-823318-4.00028-7
Sabetkish, S., & Kajbafzadeh, A. M. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-82735-9_9 · Cited by 1
Fabricating a Low-cost and Low-immune Xenogeneic Aortic Valve Bio ProsthesisPreprint
Walawalkar, S., Almelkar, S., Christoforidis, S., & Rath, S. N. (2021) · Research Square · doi:10.21203/rs.3.rs-1023576/v1
El budismo y el problema del absoluto
Ferrer, A. (2004) · Dialnet (Universidad de la Rioja)
Establishment of optimal decellularization conditions using porcine placenta
Son, J. H., Kim, D., Lee, D.-M., & Seo, B. B. (2021) · Journal of Animal Reproduciton and Biotechnology · doi:10.12750/jarb.36.4.253
Kim, B. S., & Kim, H. T. (2017) · Springer eBooks · doi:10.1007/978-981-10-2723-9_7
Urology and Nephrology: Regenerative Medicine Applications
Saba, I., Chabaud, S., Ramsay, S., Orabi, H., & Bolduc, S. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-801238-3.11016-5
Keshvari, M. A., Afshar, A., Daneshi, S., Khoradmehr, A., Baghban, M., Muhaddesi, M., Behrouzi, P., Miri, M. R., Azari, H., Nabipour, I., et al. (2021) · Research Square · doi:10.21203/rs.3.rs-229858/v1
Chandra, P. K., & Atala, A. A. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-801238-3.65846-4 · Cited by 1
Self-citationThree-Dimensional Bioprinting of Naturally Derived Protein-Based Biopolymers
Griffanti, G., & Nazhat, S. N. (2022) · ASM International eBooks · doi:10.31399/asm.hb.v23a.a0006894
A modified hydrogel production protocol to decrease cellular content
Braga, G. C. D., Camargo, C. P., Harmsen, M. C., Correia, A. T., Souza, S., Seelaender, M. C. L., Nunes, V. A., dos Santos, J. F., Neri, E. A., Valadão, I. C., et al. (2022) · Acta Cirúrgica Brasileira · doi:10.1590/acb371005
Development of an advanced multi-cellular and dynamic flow model of the human alveolar airway
Duarte, J. A. (2022) · Cronfa (Swansea University) · doi:10.23889/suthesis.62324
Engineering the (vascularized) Proximal Tubule, a Guide Towards Functionality
van Genderen, A. M. (2023) · Utrecht University Repository (Utrecht University) · doi:10.33540/1697
Production of Decellularized Tissue-Derived Materials
Ahearne, M. (2023) · Springer eBooks · doi:10.1007/978-3-030-92090-6_41-1
Evaluating different methods for kidney recellularizationPreprint
Luque-Badillo, A. C., Monjarás-Ávila, C. U., Adomat, H. H., So, A., & Chavez-Munoz, C. I. (2024) · Research Square · doi:10.21203/rs.3.rs-3943939/v1
Kidney Mesenchymal Stem Cell Differentiation: Effect of Scaffold and Basic Fibroblast Growth Factor
Keshavarz, A., Nejad, D. B., Neisi, N., Moghadam, M. T., & Mansouri, E. (2024) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2024.0066
The progress in tissue engineering of kidney
Dalal, S., Maleki, A., Mozafari, M., Saeinasab, M., & Sefat, F. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-15834-6.00002-9
Extracellular Matrix Isolation: Sources and Methods
Mendibil, U., Ruiz-Hernández, R., Arnáiz, B., Khatami, N., & Abarrategi, A. (2024) · Springer eBooks · doi:10.1007/978-3-031-56363-8_7
Digital Protocol for the Bioprinting of a Three-Dimensional Acellular Dermal Scaffold
Ijeoma, P., Ridel, A. F., & Parkar, H. (2024) · Biomedical visualization · doi:10.1007/978-3-031-68501-9_5
Biomaterials for Liver and Kidney Implants
Shokuhfar, T. (2025) · doi:10.1002/9781119851004.ch6
Belova, A. D., Nemets, E. A., Filin, D. D., Ponomareva, A. S., Кирсанова, Л. А., Basok, Y. B., & Sevastianov, V. I. (2024) · Russian Journal of Transplantology and Artificial Organs · doi:10.15825/1995-1191-2025-1-103-113
Kidney Tissue Engineering Using Decellularized Organs and Pluripotent Stem Cells
Williams, M. J. (2013) · University of Florida Digital Collections (University of Florida)
Matrix Biology of the Salivary Gland: A Guide for Tissue Engineering
Martinez, M., Wu, D., Farach‐Carson, M. C., & Harrington, D. A. (2026) · Springer eBooks · doi:10.1007/978-3-032-07458-4_8
Zhang, W., Wang, S., Sun, C., Wu, L., & (23906), D. W. (2026) · Materials Letters · doi:10.1016/j.matlet.2026.141051
Guchhait, B., Tiwari, V., Yadav, R., Pal, S., Roy, S., & Tripathi, D. M. (2026) · Journal of Controlled Release · doi:10.1016/j.jconrel.2026.115080
Reactors for tissue and organ engineering
Schüler, K., Marcelino, P., Ferreira, F. C., & Silva, J. C. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-36330-6.00009-6
Zhang, T., Xu, H., Hao, H., & An, M. (2026) · Applied Materials Today · doi:10.1016/j.apmt.2026.103444
Metadata
- Authors
- Sullivan, D. C., Mirmalek-Sani, S.-H., Deegan, D. B., Baptista, P. M., Aboushwareb, T., Atala, A., & Yoo, J. J.
- Affiliations
- Virginia Tech - Wake Forest University School of Biomedical Engineering & Sciences (US)
- Wake Forest University Health Sciences (US)
- Corresponding author
- Yoo, James J.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2012-11
- Volume
- 33
- Issue
- 31
- Pages
- 7756-7764
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W1967540288
- Cited by
- 363
- References
- 59
- Citations by year
- 2026: 7 · 2025: 14 · 2024: 15 · 2023: 17 · 2022: 18 · 2021: 43 · 2020: 28 · 2019: 23 · 2018: 30 · 2017: 33 · 2016: 28 · 2015: 42 · 2014: 38 · 2013: 23 · 2012: 2
- FWCI
- 34.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Defense (W81XWH-07-1-0718)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Keywords
- decellularization, porcine kidney, sodium dodecyl sulfate, Triton X-100, extracellular matrix preservation, renal vasculature, biocompatibility, end-stage renal disease, organ transplantation, renal cell culture
- MeSH terms
- Animals, Cells, Cultured, Detergents, Extracellular Matrix, Humans, Kidney, Perfusion, Cell Death, Tissue Engineering, Sus scrofa, Tissue Scaffolds, High-Throughput Screening Assays
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
