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Cited by 363Tissue Engineering and Regenerative Medicine3D Printing in Biomedical ResearchHydrogels: synthesis, properties, applications

The DOI matches its Crossref record; 57 of the 59 references it cites were found in a registry or an index.

Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system

Sullivan, David C., Mirmalek-Sani, Sayed-Hadi, Deegan, Daniel B., Baptista, Pedro M., Aboushwareb, Tamer, Atala, Anthony, Yoo, James J.·2012·Biomaterials
DOI 10.1016/j.biomaterials.2012.07.023
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Abstract

End-stage renal failure is a devastating disease, with donor organ transplantation as the only functional restorative treatment. The current number of donor organs meets less than one-fifth of demand, so regenerative medicine approaches have been proposed as potential therapeutic alternatives. One such approach for whole large-organ bioengineering is to combine functional renal cells with a decellularized porcine kidney scaffold. The efficacy of cellular removal and biocompatibility of the preserved porcine matrices, as well as scaffold reproducibility, are critical to the success of this approach. We evaluated the effectiveness of 0.25 and 0.5% sodium dodecyl sulfate (SDS) and 1% Triton X-100 in the decellularization of adult porcine kidneys. To perform the decellularization, a high-throughput system was designed and constructed. In this study all three methods examined showed significant cellular removal, but 0.5% SDS was the most effective detergent (<50 ng DNA/mg dry tissue). Decellularized organs retained intact microarchitecture including the renal vasculature and essential extracellular matrix components. The SDS-treated decellularized scaffolds were non-cytotoxic to primary human renal cells. This method ensures clearance of porcine cellular material (which directly impacts immunoreactivity during transplantation) and preserves the extracellular matrix and cellular compatibility of these renal scaffolds. Thus, we have developed a rapid decellularization method that can be scaled up for use in other large organs, and this represents a step toward development of a transplantable organ using tissue engineering techniques.

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  1. Baptista, 2007, Liver tissue engineering using an acellular whole organ bioscaffold with perfusion cell seeding, Tissue Eng, 13, 1721

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Metadata

Authors
Sullivan, D. C., Mirmalek-Sani, S.-H., Deegan, D. B., Baptista, P. M., Aboushwareb, T., Atala, A., & Yoo, J. J.
Affiliations
  • Virginia Tech - Wake Forest University School of Biomedical Engineering & Sciences (US)
  • Wake Forest University Health Sciences (US)
Corresponding author
Yoo, James J.
ORCID
Venue
Biomaterials
Publisher
Elsevier BV
Published
2012-11
Volume
33
Issue
31
Pages
7756-7764
ISSN
0142-9612 (ISSN-L)
DOI
10.1016/j.biomaterials.2012.07.023
OpenAlex
W1967540288
Cited by
363
References
59
Citations by year
2026: 7 · 2025: 14 · 2024: 15 · 2023: 17 · 2022: 18 · 2021: 43 · 2020: 28 · 2019: 23 · 2018: 30 · 2017: 33 · 2016: 28 · 2015: 42 · 2014: 38 · 2013: 23 · 2012: 2
FWCI
34.22
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Biomaterials (publishedVersion)
  • PubMed (publishedVersion)
Funders
U.S. Department of Defense (W81XWH-07-1-0718)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
Keywords
decellularization, porcine kidney, sodium dodecyl sulfate, Triton X-100, extracellular matrix preservation, renal vasculature, biocompatibility, end-stage renal disease, organ transplantation, renal cell culture
MeSH terms
Animals, Cells, Cultured, Detergents, Extracellular Matrix, Humans, Kidney, Perfusion, Cell Death, Tissue Engineering, Sus scrofa, Tissue Scaffolds, High-Throughput Screening Assays

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