Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Abstract
About 3,000 individuals in the United States are awaiting a donor heart; worldwide, 22 million individuals are living with heart failure. A bioartificial heart is a theoretical alternative to transplantation or mechanical left ventricular support. Generating a bioartificial heart requires engineering of cardiac architecture, appropriate cellular constituents and pump function. We decellularized hearts by coronary perfusion with detergents, preserved the underlying extracellular matrix, and produced an acellular, perfusable vascular architecture, competent acellular valves and intact chamber geometry. To mimic cardiac cell composition, we reseeded these constructs with cardiac or endothelial cells. To establish function, we maintained eight constructs for up to 28 d by coronary perfusion in a bioreactor that simulated cardiac physiology. By day 4, we observed macroscopic contractions. By day 8, under physiological load and electrical stimulation, constructs could generate pump function (equivalent to about 2% of adult or 25% of 16-week fetal heart function) in a modified working heart preparation.
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Ott's group created contractile 3D cardiac tissues from decellularized matrices and iPSC-derived cardiomyocytes, offering potential for heart repair.
“Recellularization, the reintroduction of cells into ECM scaffolds to form functional tissues, has been exemplified by Ott's group, which created contractile 3D cardiac tissues from decellularized matrices and iPSC‐derived cardiomyocytes, offering potential for heart repair.[ 148 ]”
From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [148]
Upon repopulation with cardiomyocytes or endothelial cells and subsequent culture, decellularized rat heart constructs regain cardiac function.
“Upon repopulation with cardiomyocytes or endothelial cells and subsequent culture, these constructs regain cardiac function.”
From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [148]
Ott et al. successfully optimized decellularization of rat hearts, creating scaffolds with preserved vasculature, valves, and chambers.
“Ott et al. successfully optimized the decellularization of rat hearts, creating scaffolds with preserved vasculature, valves, and chambers.[ 148 ]”
From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [148]
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The bioengineered cardiac left ventricle
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Li, S., Liang, C., Jiang, W., Deng, J., Gu, R., Li, W., Tian, F., Tang, L., & Sun, H. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c01610 · Cited by 12 · see citing works
- Authors
- Ott, H. C., Matthiesen, T. S., Goh, S.-K., Black, L. D., Kren, S. M., Netoff, T. I., & Taylor, D. A.
- Affiliations
- Harvard University (US)
- University of Minnesota (US)
- Corresponding author
- Taylor, Doris A
- Venue
- Nature Medicine
- Published
- 2008-01-13
- Volume
- 14
- Issue
- 2
- Pages
- 213-221
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- OpenAlex
- W2101228997
- Cited by
- 2,650
- References
- 3
- Citations by year
- 2026: 55 · 2025: 55 · 2024: 83 · 2023: 109 · 2022: 103 · 2021: 160 · 2020: 155 · 2019: 163 · 2018: 158 · 2017: 177 · 2016: 202 · 2015: 213 · 2014: 222 · 2013: 194 · 2012: 180
- FWCI
- 146.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Minnesota
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Mechanical Circulatory Support Devices
- Keywords
- bioartificial heart, extracellular matrix, coronary perfusion, endothelial cells, cardiac pump function, bioreactors, heart failure, left ventricular support, cardiac tissue engineering
- MeSH terms
- Animals, Cadaver, Extracellular Matrix, Heart, Artificial, Male, Myocardium, Perfusion, Rats, Inbred F344, Bioartificial Organs, Tissue Engineering, Endothelial Cells, Rats
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