Aligned neurite outgrowth and directed cell migration in self-assembled monodomain gels
Open accessAbstract
Regeneration of neural tissues will require regrowth of axons lost due to trauma or degeneration to reestablish neuronal connectivity. One approach toward this goal is to provide directional cues to neurons that can promote and guide neurite growth. Our laboratory previously reported the formation of aligned monodomain gels of peptide amphiphile (PA) nanofibers over macroscopic length scales. In this work, we modified these aligned scaffolds specifically to support neural cell growth and function. This was achieved by displaying extracellular matrix (ECM) derived bioactive peptide epitopes on the surface of aligned nanofibers of the monodomain gel. Presentation of IKVAV or RGDS epitopes enhanced the growth of neurites from neurons encapsulated in the scaffold, while the alignment guided these neurites along the direction of the nanofibers. After two weeks of culture in the scaffold, neurons displayed spontaneous electrical activity and established synaptic connections. Scaffolds encapsulating neural progenitor cells were formed in situ within the spinal cord and resulted in the growth of oriented processes in vivo. Moreover, dorsal root ganglion (DRG) cells demonstrated extensive migration inside the scaffold, with the direction of their movement guided by fiber orientation. The bioactive and macroscopically aligned scaffold investigated here and similar variants can potentially be tailored for use in neural tissue regeneration.
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Similar work has shown promise in stem cell differentiation and neural regeneration following spinal injury in vivo, further demonstrating the utility of peptide-based materials in cell culture.
“Similar work has shown promise in stem cell differentiation and neural regeneration following spinal injury in vivo200–202, further demonstrating the utility of peptide-based materials in cell culture.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [202]
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Towards organoid culture without Matrigel
Kozlowski, M. T., Crook, C. J., & Ku, H. T. (2021) · Communications Biology · doi:10.1038/s42003-021-02910-8 · Cited by 409 · see citing works
- Authors
- Berns, E. J., Sur, S., Pan, L., Goldberger, J. E., Suresh, S., Zhang, S., Kessler, J. A., & Stupp, S. I.
- Affiliations
- Northwestern University (US)
- Corresponding author
- Stupp, Samuel I.
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2014-01
- Volume
- 35
- Issue
- 1
- Pages
- 185-195
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC3865987
- OpenAlex
- W1992603067
- Cited by
- 208
- References
- 43
- Citations by year
- 2026: 11 · 2025: 11 · 2024: 9 · 2023: 17 · 2022: 20 · 2021: 11 · 2020: 21 · 2019: 10 · 2018: 23 · 2017: 22 · 2016: 22 · 2015: 17 · 2014: 13 · 2013: 1
- FWCI
- 6.74
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biomaterials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health/National Institute Of Biomedical Imaging And Bioengineering
- Topics
- Physical Sciences › Materials Science › Biomaterials › Supramolecular Self-Assembly in Materials
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Keywords
- neurite outgrowth, directed cell migration, peptide amphiphile nanofibers, neural tissue regeneration, RGDS, neural progenitor cells, dorsal root ganglion neurons, spinal cord, synaptic connectivity, axon guidance
- MeSH terms
- Animals, Cell Movement, Cells, Cultured, Gels, Microscopy, Electron, Scanning, Synaptic Transmission, Oligopeptides, Neurites, Patch-Clamp Techniques, Microscopy, Electron, Transmission, Mice, Tissue Scaffolds, Nanofibers
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