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Cited by 117Tissue Engineering and Regenerative Medicine3D Printing in Biomedical ResearchMesenchymal stem cell research

Cryo-chemical decellularization of the whole liver for mesenchymal stem cells-based functional hepatic tissue engineering

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Jiang, Wei-Cheng, Cheng, Yu-Hao, Yen, Meng-Hua, Chang, Yin, Yang, Vincent W., Lee, Oscar K.·2014·Biomaterials
DOI 10.1016/j.biomaterials.2014.01.024PMC PMC4678102
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Abstract

Liver transplantation is the ultimate treatment for severe hepatic failure to date. However, the limited supply of donor organs has severely hampered this treatment. So far, great potentials of using mesenchymal stem cells (MSCs) to replenish the hepatic cell population have been shown; nevertheless, there still is a lack of an optimal three-dimensional scaffold for generation of well-transplantable hepatic tissues. In this study, we utilized a cryo-chemical decellularization method which combines physical and chemical approach to generate acellular liver scaffolds (ALS) from the whole liver. The produced ALS provides a biomimetic three-dimensional environment to support hepatic differentiation of MSCs, evidenced by expression of hepatic-associated genes and marker protein, glycogen storage, albumin secretion, and urea production. It is also found that hepatic differentiation of MSCs within the ALS is much more efficient than two-dimensional culture in vitro. Importantly, the hepatic-like tissues (HLT) generated by repopulating ALS with MSCs are able to act as functional grafts and rescue lethal hepatic failure after transplantation in vivo. In summary, the cryo-chemical method used in this study is suitable for decellularization of liver and create acellular scaffolds that can support hepatic differentiation of MSCs and be used to fabricate functional tissue-engineered liver constructs.

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  • Jiang et al. found that the three-dimensional environment of decellularized liver supported hepatic differentiation of MSCs, as evidenced by higher expression levels of hepatic-specific genes and marker proteins, increased glycogen storage, albumin secretion, and urea production compared to cells differentiated in 2D cultures.

    “The three-dimensional environment of the decellularized liver supported the hepatic differentiation of MSCs, as evidenced by higher expression levels of hepatic-specific genes and marker proteins, increased glycogen storage, albumin secretion, and urea production compared to cells differentiated in 2D cultures.”

    From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [211]

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Authors
Jiang, W.-C., Cheng, Y.-H., Yen, M.-H., Chang, Y., Yang, V. W., & Lee, O. K.
Affiliations
  • National Yang Ming University (TW)
  • Stony Brook School (US)
  • Taipei Veterans General Hospital (TW)
Corresponding author
Lee, Oscar K.
ORCID
Venue
Biomaterials
Publisher
Elsevier BV
Published
2014-04
Volume
35
Issue
11
Pages
3607-3617
ISSN
0142-9612 (ISSN-L)
DOI
10.1016/j.biomaterials.2014.01.024
PMC
PMC4678102
OpenAlex
W2067425028
Cited by
117
References
3
Citations by year
2026: 3 · 2025: 4 · 2024: 4 · 2023: 7 · 2022: 5 · 2021: 17 · 2020: 10 · 2019: 11 · 2018: 11 · 2017: 12 · 2016: 12 · 2015: 16 · 2014: 4
FWCI
14.55
Type
journal article
Language
en
Open access
Licence
Locations
  • Biomaterials (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Taiwan National Science Council I-RiCE Program (NSC101-2911-I-009-101)
  • Taipei Veterans General Hospital (VGH102E1-007, VGH102C-001)
  • National Science Council, Taiwan (NSC102-2120-M-010-002, NSC100-2314-B-010-030-MY3, NSC102-2321-B-010-008)
  • Ministry of Economic Affairs, Taiwan (102-EC-17-A-17-S1-203)
  • Ministry of Education
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
Keywords
mesenchymal stem cells, hepatic differentiation, liver transplantation, liver failure, 3D scaffolds, biomimetic scaffolds
MeSH terms
Animals, Cell Differentiation, Cold Temperature, Gene Expression Regulation, Liver, Mice, Inbred BALB C, Biomarkers, Liver Transplantation, Mice, SCID, Tissue Engineering, Mice, Tissue Scaffolds, Mesenchymal Stem Cells, Real-Time Polymerase Chain Reaction

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