Imaging γ-Glutamyltranspeptidase for tumor identification and resection guidance via enzyme-triggered fluorescent probe
Open accessAbstract
Development of high selectivity, accurate targeted and noninvasive fluorescent probe for monitoring specific enzyme activity associated with the tumor is urgent needed for early diagnosis of cancer and clinical fluorescence interventional resection guidance treatment. Owing to the invasion of malignant tumor cells, tumor cells are mixed with normal cells in the actual tumor location, which make it quite difficult for clinician to diagnose early diagnosis of tumor as well as resection of tumor. To overcome aforementioned obstacle, herein, an ingenious enzyme-activated one and two-photon fluorescent probe TCF-GGT was constructed and synthesized including γ-GGT enzyme specific identification site and far-red fluorophore for imaging. Under simulative physiological condition, probe TCF-GGT demonstrated high selectivity, sensitivity (DL 0.014 mU/mL), rapid response (T e 14 min) for the detection of γ-GGT enzyme. By virtue of its biocompatibility, probe was employed for the identification of ovarian cancer cells (A2780 cells) from normal cells (NIH-3T3 cells), particularly in mixed cultivation dish (simulate the actual environment of tumor) through 2D&3D fluorescence imaging with "dual" mark (Nucleic acid labeling used by Hoechst 33342 dye and γ-GGT enzyme labeling used by probe TCF-GGT) for the first time. Probe TCF-GGT could be visualize endogenous γ-GGT activity in HepG-2 cells and zebrafish on the two-photon confocal platform, which is conduce to estimate the inhibitor of γ-GGT enzyme in vivo. Through NaBu (a potential anticancer drug) stimulation, the changes of γ-GGT activity were observed in living MCF-7 cells by using this probe. More importantly, the deep tissue penetration ability of far-red fluorescence allowed the two-photon fluorescent probe TCF-GGT to real-time track γ-GGT activity in tissue slices and tumor xenotransplantation model of mice by the tail vein injection, which showed that this enzyme-triggered fluorogenic probe would be a potential tool for preclinical applications of tumor resection.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Hyaluronidase, γ-Glutamyltranspeptidase, prostate-specific antigen, Trysin, β-galactosidase, Azoreductase, etc. are also used in enzyme-stimulated response smart nano-delivery systems.
“In addition, hyaluronidase, γ-Glutamyltranspeptidase, prostate-specific antigen, Trysin, β-galactosidase, Azoreductase, etc. are also used in enzyme-stimulated response smart nano-delivery systems.157–162”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [157]
Cites
Cancer statistics in China, 2015
Chen, W., Zheng, R., Baade, P. D., Zhang, S., Zeng, H., Bray, F., Jemal, A., Yu, X. Q., & He, J. (2016) · CA: A Cancer Journal for Clinicians · doi:10.3322/caac.21338 · Cited by 17,038 · see citing works
Siegel, R. L., Miller, K. D., & Jemal, A. (2016) · CA: A Cancer Journal for Clinicians · doi:10.3322/caac.21332 · Cited by 16,003 · see citing works
Molecular logic gates: the past, present and future
Erbaş-Çakmak, S., Kölemen, S., Sedgwick, A. C., et al. (2018) · Chemical Society Reviews · doi:10.1039/c7cs00491e · Cited by 668 · see citing works
Gu, K., Xu, Y., Li, H., Guo, Z., Zhu, S., Zhu, S., Shi, P., James, T. D., Tian, H., & Zhu, W. (2016) · Journal of the American Chemical Society · doi:10.1021/jacs.6b01705 · Cited by 499 · see citing works
Self-citationDual-Site Fluorescent Probe for Visualizing the Metabolism of Cys in Living Cells
Yue, Y., Huo, F., Ning, P., Zhang, Y., Chao, J., Meng, X., & Yin, C. (2017) · Journal of the American Chemical Society · doi:10.1021/jacs.6b12845 · Cited by 497 · see citing works
Rapid Cancer Detection by Topically Spraying a γ-Glutamyltranspeptidase–Activated Fluorescent Probe
Urano, Y., Sakabe, M., Kosaka, N., Ogawa, M., Mitsunaga, M., Asanuma, D., Kamiya, M., Young, M. R., Nagano, T., Choyke, P. L., et al. (2011) · Science Translational Medicine · doi:10.1126/scitranslmed.3002823 · Cited by 468 · see citing works
Preoperative assessment enables the early diagnosis and successful treatment of lymphedema
Stout, N. L., Pfalzer, L., McGarvey, C. L., Springer, B. A., Gerber, L. H., & Soballe, P. W. (2008) · Cancer · doi:10.1002/cncr.23494 · Cited by 468 · see citing works
Asanuma, D., Sakabe, M., Kamiya, M., Yamamoto, K., Hiratake, J., Ogawa, M., Kosaka, N., Choyke, P. L., Nagano, T., Kobayashi, H., et al. (2015) · Nature Communications · doi:10.1038/ncomms7463 · Cited by 420 · see citing works
Orłowski, M., & Meister, A. (1963) · Biochimica et Biophysica Acta · doi:10.1016/0006-3002(63)90348-2 · Cited by 381 · see citing works
Orłowski, M., & Meister, A. (1963) · Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects · doi:10.1016/0926-6569(63)90197-4 · Cited by 363 · see citing works
Li, X., Kim, C., Lee, D., Lee, D., Chung, H., Kim, G., Heo, S.-H., Kim, C., Hong, K., & Yoon, J. (2017) · Journal of the American Chemical Society · doi:10.1021/jacs.7b05916 · Cited by 343 · see citing works
An Off–On COX-2-Specific Fluorescent Probe: Targeting the Golgi Apparatus of Cancer Cells
Zhang, H., Fan, J., Wang, J., Zhang, S., Dou, B., & Peng, X. (2013) · Journal of the American Chemical Society · doi:10.1021/ja4056905 · Cited by 324 · see citing works
Self-citationFluorogenic reaction-based prodrug conjugates as targeted cancer theranostics
Lee, M. H., Sharma, A. K., Chang, M. J., Lee, J., Son, S., Sessler, J. L., Kang, C., & Kim, J. S. (2017) · Chemical Society Reviews · doi:10.1039/c7cs00557a · Cited by 318 · see citing works
Expression of γ-glutamyltransferase in cancer cells and its significance in drug resistance
Alfonso Pompella, C., Tata, V. D., Paolicchi, A., & Zunino, F. (2005) · Biochemical Pharmacology · doi:10.1016/j.bcp.2005.10.005 · Cited by 280 · see citing works
Hanigan, M. H., & Ricketts, W. A. (1993) · Biochemistry · doi:10.1021/bi00075a026 · Cited by 273 · see citing works
Disulfide-Based Multifunctional Conjugates for Targeted Theranostic Drug Delivery
Lee, M. H., Sessler, J. L., & Kim, J. S. (2015) · Accounts of Chemical Research · doi:10.1021/acs.accounts.5b00406 · Cited by 255 · see citing works
Dong, B., Song, X., Kong, X., Wang, C., Tang, Y., Liu, Y., & Lin, W. (2016) · Advanced Materials · doi:10.1002/adma.201602939 · Cited by 217 · see citing works
Wang, F., Zhu, Y. J., Zhou, L., Pan, L., Cui, Z., Fei, Q., Luo, S., Pan, D., Huang, Q., Wang, R., et al. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201502899 · Cited by 201 · see citing works
Liu, H., Hu, X., Li, K., Liu, Y., Rong, Q., Zhu, L., Yuan, L., Qu, F., Zhang, X., & Tan, W. (2017) · Chemical Science · doi:10.1039/c7sc03454g · Cited by 201 · see citing works
Lei, Z., Li, X., Luo, X., He, H., Zheng, J., Qian, X., & Yang, Y. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201612301 · Cited by 177 · see citing works
Jun, Y. W., Kim, H. R., Reo, Y. J., Dai, M., & Ahn, K. H. (2017) · Chemical Science · doi:10.1039/c7sc03362a · Cited by 176 · see citing works
Liu, K., Kong, X., Ma, Y., et al. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201707518 · Cited by 173 · see citing works
Ye, M., Wang, X., Tang, J., Guo, Z., Shen, Y., Tian, H., & Zhu, W. (2016) · Chemical Science · doi:10.1039/c6sc00970k · Cited by 155 · see citing works
Zhang, P., Jiang, X., Nie, X., Huang, Y. X., Zeng, F., Xia, X., & Wu, S. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.11.047 · Cited by 147 · see citing works
Mao, Z., Feng, W., Li, Z., Zeng, L., Lv, W., & Liu, Z. (2016) · Chemical Science · doi:10.1039/c6sc01313a · Cited by 136 · see citing works
Kim, E.-J., Kumar, R., Sharma, A., Yoon, B., Kim, H. M., Lee, H., Hong, K. S., & Kim, J. S. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.01.009 · Cited by 129 · see citing works
A Strategy for Specific Fluorescence Imaging of Monoamine Oxidase A in Living Cells
Wu, X., Shi, W. S., Li, X., & Ma, H. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201708428 · Cited by 123 · see citing works
A fluorescent probe for H 2 S in vivo with fast response and high sensitivity
Li, H. D., Yao, Q., Fan, J., et al. (2015) · Chemical Communications · doi:10.1039/c5cc06612c · Cited by 122 · see citing works
Self-citationSedgwick, A. C., Han, H., Gardiner, J. E., Bull, S. D., He, X., & James, T. D. (2017) · Chemical Communications · doi:10.1039/c7cc07845e · Cited by 122 · see citing works
A two-photon NIR-to-NIR fluorescent probe for imaging hydrogen peroxide in living cells
Li, H., Yao, Q., Fan, J., Du, J., Wang, J., & Peng, X. (2017) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2017.03.039 · Cited by 116 · see citing works
Self-citationLi, L., Shi, W. S., Wu, X., Gong, Q., Li, X., & Ma, H. (2016) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2016.03.021 · Cited by 107 · see citing works
Rao, M. S., Tatematsu, M., Subbarao, V., et al. (1986) · PubMed · Cited by 98 · see citing works
Fluorescent Probes Containing Selenium as a Guest or Host
Wu, D., Chen, L., Kwon, N., & Yoon, J. (2016) · Chem · doi:10.1016/j.chempr.2016.10.005 · Cited by 98 · see citing works
A Fluorescent Probe for Ratiometric Imaging of SO2 Derivatives in Mitochondria of Living Cells
Li, H., Yao, Q., Fan, J., et al. (2016) · Industrial & Engineering Chemistry Research · doi:10.1021/acs.iecr.5b04530 · Cited by 92 · see citing works
Self-citationLi, L., Shi, W. S., Wang, Z., Gong, Q., & Ma, H. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b01535 · Cited by 91 · see citing works
Ilio, C. D., Polidoro, G., Arduini, A., et al. (1983) · Biochemical Medicine · doi:10.1016/0006-2944(83)90034-0 · Cited by 90 · see citing works
Gao, M., Wang, R., Yu, F., & Chen, L. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.01.011 · Cited by 85 · see citing works
He, X., Xu, Y., Shi, W. S., & Ma, H. (2017) · Analytical Chemistry · doi:10.1021/acs.analchem.7b00021 · Cited by 84 · see citing works
Wang, F., Xu, G., Gu, X., Wang, Z., Wang, Z., Shi, B., Lu, C., Gong, X., & Zhao, C. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.01.009 · Cited by 78 · see citing works
Localization and significance of gamma-glutamyltranspeptidase in normal and neoplastic mouse skin.
Young, L. M. D., Richards, W. L., Bonzelet, W., et al. (1978) · PubMed · Cited by 77 · see citing works
Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro
Wang, H., Huang, X., Shen, P., Li, L., XUE, H.-T., & Ji, G. (2013) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2013.1285 · Cited by 71 · see citing works
An NIR fluorescent probe of uric HSA for renal diseases warning
Li, H., Yao, Q., Fan, J., et al. (2016) · Dyes and Pigments · doi:10.1016/j.dyepig.2016.05.039 · Cited by 70 · see citing works
Self-citationLi, H., Zhou, X. Y., Fan, J., et al. (2017) · Sensors and Actuators B Chemical · doi:10.1016/j.snb.2017.07.082 · Cited by 65 · see citing works
Self-citationWang, Y., Shi, Y., Wang, Z., Zhu, Z., Zhao, X., Nie, H., Qian, J., Qin, A., Sun, J. Z., & Tang, B. Z. (2016) · Chemistry - A European Journal · doi:10.1002/chem.201600125 · Cited by 64 · see citing works
Shinozuka, H., Sells, M. A., Katyal, S. L., et al. (1979) · PubMed · Cited by 63 · see citing works
Zhou, Q., Xiang, L., Shao, J., Hu, R., Lu, Y., Yao, H. P., & Dai, L. (2006) · Journal of Cellular Biochemistry · doi:10.1002/jcb.20970 · Cited by 62 · see citing works
Park, S., Lim, S.-Y., Bae, S. M., Kim, S.-Y., Myung, S., & Kim, H.-J. (2016) · ACS Sensors · doi:10.1021/acssensors.5b00249 · Cited by 53 · see citing works
Zhang, H., Liu, J., Wang, L., et al. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2017.12.013 · Cited by 52 · see citing works
Zhang, H., Wang, K., Xuan, X., Lv, Q., Nie, Y., & Guo, H. (2016) · Chemical Communications · doi:10.1039/c6cc02290a · Cited by 51 · see citing works
Park, Y., Ryu, Y., Wang, T., Jung, Y., Kim, S., Hwang, S., Park, J., Bae, D., Kim, J., Moon, H., et al. (2017) · Advanced Functional Materials · doi:10.1002/adfm.201703450 · Cited by 49 · see citing works
Strasak, A. M., Goebel, G., Concin, H., Pfeiffer, R. M., Brant, L. J., Nagel, G., Oberaigner, W., Concin, N., Diem, G., Ruttmann, E., et al. (2010) · Cancer Research · doi:10.1158/0008-5472.can-09-3197 · Cited by 46 · see citing works
Fan, J., Guo, S., Wang, S., Kang, Y., Yao, Q., Wang, J., Gao, X., Wang, H., Du, J., & Peng, X. (2017) · Chemical Communications · doi:10.1039/c7cc01798g · Cited by 43 · see citing works
Self-citationPark, S., Bae, D. J., Ryu, Y., et al. (2016) · Chemical Communications · doi:10.1039/c6cc05573g · Cited by 38 · see citing works
A new useful modification for the determination of γ-glutamyltranspeptidase activity
Kulhánek, V., & Dimov, D. M. (1966) · Clinica Chimica Acta · doi:10.1016/0009-8981(66)90186-0 · Cited by 37 · see citing works
Kiuchi, K., Kiuchi, K., Nagatsu, T., et al. (1986) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)95820-0 · Cited by 36 · see citing works
Wang, P., Zhang, J., Liu, H., Hu, X., Feng, L., Yin, X., & Zhang, X. (2017) · The Analyst · doi:10.1039/c7an00229g · Cited by 32 · see citing works
Lei, Z., Li, X., Luo, X. L., et al. (2017) · Angewandte Chemie · doi:10.1002/ange.201612301 · Cited by 29 · see citing works
Chen, X., Lim, C. S., Lee, D., Lee, S., Park, S. J., Kim, H. M., & Yoon, J. (2017) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2017.01.042 · Cited by 26 · see citing works
Kim, H. R., Kumar, R., Kim, W., et al. (2016) · Chemical Communications · doi:10.1039/c6cc02330d · Cited by 20 · see citing works
Kwiecień, I., Rokita, H., Lorenc‐Koci, E., Sokołowska, M. M., & Włodek, L. B. (2007) · Fundamental and Clinical Pharmacology · doi:10.1111/j.1472-8206.2006.00458.x · Cited by 17 · see citing works
Visible Fluorescence Detection of Type III Protein Secretion from Bacterial Pathogens
Yount, J. S., Tsou, L. K., Dossa, P. D., Kullas, A. L., van der Velden, A. W. M., & Hang, H. C. (2010) · Journal of the American Chemical Society · doi:10.1021/ja102257v · Cited by 14 · see citing works
A proton-activatable aminated-chrysophanol sensitizer for photodynamic therapy
Bian, Y., Li, M., Fan, J., et al. (2017) · Dyes and Pigments · doi:10.1016/j.dyepig.2017.08.029 · Cited by 14 · see citing works
Self-citationFluorescent Probes for the Recognition of Hypochlorous Acid
Li, H., Fan, J., & Peng, X. (2017) · Huaxue jinzhan · doi:10.7536/pc161210 · Cited by 14 · see citing works
Self-citationWajchenberg (2000) · doi:10.1002/(sici)1097-0142(20000215)88:4
Flagged first, then by citations
Cited by
Sorted by citations
Smart nanoparticles for cancer therapy
Sun, L., Liu, H., Ye, Y., Lei, Y., Islam, R., Tan, S., Tong, R., Miao, Y.-B., & Cai, L. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01642-x · Cited by 1,073 · see citing works
Recent progresses in small-molecule enzymatic fluorescent probes for cancer imaging
Liu, H.-W., Chen, L., Xu, C., Li, Z., Zhang, H., Zhang, X.-B., & Tan, W. (2018) · Chemical Society Reviews · doi:10.1039/c7cs00862g · Cited by 948 · see citing works
- Authors
- Li, H., Yao, Q., Xu, F., Xu, N., Duan, R., Long, S., Fan, J., Du, J., Wang, J., & Peng, X.
- Affiliations
- Dalian University of Technology (CN)
- Corresponding author
- Peng, Xiaojun
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2018-10
- Volume
- 179
- Pages
- 1-14
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2808778980
- Cited by
- 109
- References
- 64
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 13 · 2023: 19 · 2022: 16 · 2021: 16 · 2020: 19 · 2019: 8 · 2018: 5
- FWCI
- 5.18
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (am, 270 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Biomaterials (acceptedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (21421005, 21576037, U1608222, 21422601, 21376039)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Sulfur Compounds in Biology
- Keywords
- gamma-glutamyltranspeptidase, far-red fluorescence, two-photon imaging, tumor resection guidance, ovarian cancer, HepG2 cells, zebrafish, tumor xenografts, enzyme activity imaging, NABU, MCF-7 cells, tumor cell identification
- MeSH terms
- Animals, Cell Survival, Female, Fluorescent Dyes, gamma-Glutamyltransferase, HeLa Cells, Humans, Zebrafish, NIH 3T3 Cells, Mice, Hep G2 Cells, Optical Imaging, MCF-7 Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
