The complete enzymatic saccharification of agarose and its application to simultaneous saccharification and fermentation of agarose for ethanol production
Abstract
A sugar platform equipped with acetic acid, multiple agarases and neoagarobiose hydrolase (NABH) converted recalcitrant agar polysaccharide into monosugars, which was evaluated by simultaneous saccharification and fermentation (SSF). The sugar platform was divided into chemical liquefaction and enzymatic saccharification. The chemical liquefaction was carried out in mild conditions (using a dilute acetic acid at 80°C for 1-6h) to avoid the production of fermentation inhibitors and hence the highest degree of liquefaction of 95.6% (w/w) was obtained. We mimicked the natural agarolytic pathway using three microbial agarases (Aga16B, Aga50D and DagA) and NABH, and the enzyme system converted 79.1% of agarose to monosugars. The chemical liquefaction and SSF of 30 g/l agarose resulted in 4.4 g/l ethanol concentration and 49.3% of the theoretical ethanol yield to d-galactose. This is the first report on the complete enzymatic conversion of agarose into its monosugars and the SSF of agarose into ethanol.
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To increase the yield, an agarose acid and heat pretreatment step, also known as chemical liquefaction, has been introduced preceding the enzymatic hydrolysis step.
“To increase the yield, an agarose acid and heat pretreatment step (also known as chemical liquefaction) has been introduced preceding the enzymatic hydrolysis step.”
From The Enzymatic Conversion of Major Algal and Cyanobacterial Carbohydrates to Bioethanol (2016) · cites this paper as Kim et al., 2012
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Kim, D. H., Park, S. Y., & Kim, K. H. (2022) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-022-12274-6 · Cited by 3 · see citing works
Self-citationShen, J. F., Zhou, M., Dan, M., Zheng, Y., Zhao, G., & Wang, D. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.135682 · Cited by 2 · see citing works
Lee, S., & Kim, Y.-H. (2020) · Microbiology and Biotechnology Letters · doi:10.4014/mbl.1909.09008 · Cited by 2 · see citing works
Self-citationGarcia‐Ruiz, E., Badur, A. H., Rao, C. V., & Zhao, H. (2016) · Green chemistry and sustainable technology · doi:10.1007/978-3-662-53704-6_5 · Cited by 2 · see citing works
Torulaspora quercuum shows great potential for bioethanol production from macroalgal hydrolysate
Morimoto, K., Chiou, T., & Konishi, M. (2022) · Bioresource Technology Reports · doi:10.1016/j.biteb.2022.100952 · Cited by 1 · see citing works
Dutschei, T., Zühlke, M.-K., Welsch, N., Eisenack, T., Hilkmann, M., Krull, J., Stühle, C., Brott, S., Dürwald, A., Reisky, L., et al. (2022) · Figshare · doi:10.6084/m9.figshare.21308677
Jiang, C., & Mao, X. (2025) · Springer eBooks · doi:10.1007/978-981-96-9517-1_7
- Authors
- Kim, H. T., Lee, S., Kim, K. H., & Choi, I.-G.
- Affiliations
- Korea University (KR)
- Corresponding author
- Choi, In-Geol
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2012-03
- Volume
- 107
- Pages
- 301-306
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2007656033
- Cited by
- 81
- References
- 31
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 3 · 2022: 7 · 2021: 4 · 2020: 9 · 2019: 6 · 2018: 8 · 2017: 9 · 2016: 10 · 2015: 6 · 2014: 7 · 2013: 4 · 2012: 2
- FWCI
- 2.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Science and Technology (2011-0015629)
- National Research Foundation
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Keywords
- agarose, enzymatic saccharification, simultaneous saccharification and fermentation, ethanol production, agarase, galactose, fermentation inhibitors, DAGA
- MeSH terms
- Ethanol, Base Sequence, Carbohydrates, Cloning, Molecular, Enzymes, Fermentation, Sepharose, DNA Primers
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