Cited by 81Biofuel production and bioconversionEnzyme Production and CharacterizationEnzyme Catalysis and Immobilization

The complete enzymatic saccharification of agarose and its application to simultaneous saccharification and fermentation of agarose for ethanol production

Kim, Hee Taek, Lee, Saeyoung, Kim, Kyoung Heon, Choi, In-Geol·2012·Bioresource Technology

Abstract

A sugar platform equipped with acetic acid, multiple agarases and neoagarobiose hydrolase (NABH) converted recalcitrant agar polysaccharide into monosugars, which was evaluated by simultaneous saccharification and fermentation (SSF). The sugar platform was divided into chemical liquefaction and enzymatic saccharification. The chemical liquefaction was carried out in mild conditions (using a dilute acetic acid at 80°C for 1-6h) to avoid the production of fermentation inhibitors and hence the highest degree of liquefaction of 95.6% (w/w) was obtained. We mimicked the natural agarolytic pathway using three microbial agarases (Aga16B, Aga50D and DagA) and NABH, and the enzyme system converted 79.1% of agarose to monosugars. The chemical liquefaction and SSF of 30 g/l agarose resulted in 4.4 g/l ethanol concentration and 49.3% of the theoretical ethanol yield to d-galactose. This is the first report on the complete enzymatic conversion of agarose into its monosugars and the SSF of agarose into ethanol.

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Crossref · 10.1016/j.biortech.2011.11.120

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29 of 29 references verified0 citation errors · 0 not checkable

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  • To increase the yield, an agarose acid and heat pretreatment step, also known as chemical liquefaction, has been introduced preceding the enzymatic hydrolysis step.

    “To increase the yield, an agarose acid and heat pretreatment step (also known as chemical liquefaction) has been introduced preceding the enzymatic hydrolysis step.”

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Authors
Kim, H. T., Lee, S., Kim, K. H., & Choi, I.-G.
Affiliations
Korea University (KR)
Corresponding author
Choi, In-Geol
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2012-03
Volume
107
Pages
301-306
ISSN
0960-8524 (ISSN-L)
OpenAlex
W2007656033
Cited by
81
References
31
Citations by year
2025: 4 · 2024: 2 · 2023: 3 · 2022: 7 · 2021: 4 · 2020: 9 · 2019: 6 · 2018: 8 · 2017: 9 · 2016: 10 · 2015: 6 · 2014: 7 · 2013: 4 · 2012: 2
FWCI
2.46
Type
journal article
Language
en
Open access
closed
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Education, Science and Technology (2011-0015629)
  • National Research Foundation
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
Keywords
agarose, enzymatic saccharification, simultaneous saccharification and fermentation, ethanol production, agarase, galactose, fermentation inhibitors, DAGA
MeSH terms
Ethanol, Base Sequence, Carbohydrates, Cloning, Molecular, Enzymes, Fermentation, Sepharose, DNA Primers

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