Cited by 238Algal biology and biofuel productionCoagulation and Flocculation StudiesAquatic Ecosystems and Phytoplankton Dynamics

Microalgae-associated bacteria play a key role in the flocculation of Chlorella vulgaris

Lee, Jimin, Cho, Dae-Hyun, Ramanan, Rishiram, Kim, Byung-Hyuk, Oh, Hee-Mock, Kim, Hee-Sik·2013·Bioresource Technology

Abstract

Flocculation is most preferred method for harvesting microalgae, however, the role of bacteria in microalgal flocculation process is still unknown. This study proves that bacteria play a profound role in flocculating by increasing the floc size resulting in sedimentation of microalgae. A flocculating activity of 94% was achieved with xenic Chlorella vulgaris culture as compared to 2% achieved with axenic culture. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene of xenic C. vulgaris culture revealed the presence of Flavobacterium sp., Terrimonas sp., Sphingobacterium sp., Rhizobium sp. and Hyphomonas sp. as microalgae-associated bacteria. However when Flavobacterium, Terrimonas, Sphingobacterium were eliminated by fluorescence activated cell sorter (FACS), flocculating activity reduced to 3%. Further studies with cell free extracts also suggest that bacterial extracellular substances might also have a role in enhancing flocculation. We conclude that the collective presence of certain bacteria is the determining factor in flocculation of C. vulgaris.

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Crossref · 10.1016/j.biortech.2012.11.130

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Authors
Lee, J., Cho, D.-H., Ramanan, R., Kim, B.-H., Oh, H.-M., & Kim, H.-S.
Affiliations
Korea Research Institute of Bioscience and Biotechnology (KR)
Corresponding author
Kim, Hee-Sik
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2013-03
Volume
131
Pages
195-201
ISSN
0960-8524 (ISSN-L)
OpenAlex
W2069771863
Cited by
238
References
39
Citations by year
2026: 8 · 2025: 13 · 2024: 19 · 2023: 21 · 2022: 20 · 2021: 27 · 2020: 29 · 2019: 21 · 2018: 16 · 2017: 17 · 2016: 9 · 2015: 18 · 2014: 17 · 2013: 3
FWCI
4.67
Type
journal article
Language
en
Open access
closed
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
  • KRIBB Repository (submittedVersion)
Funders
Korea Research Institute of Bioscience and Biotechnology
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Physical Sciences › Environmental Science › Water Science and Technology › Coagulation and Flocculation Studies
  • Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
Keywords
Chlorella vulgaris, microalgae harvesting, floc size, sedimentation, Flavobacterium, Terrimonas, Sphingobacterium, Rhizobium, Hyphomonas, xenic culture, axenic culture
MeSH terms
Flocculation, Bioreactors, Chlorella vulgaris, Microalgae

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