Modeling of rhythmic behavior in neutral lipid production due to continuous supply of limited nitrogen: Mutual growth and lipid accumulation in microalgae
Open accessAbstract
The relative effects of three precise nitrogen limitation regimes on green micro-algae were assessed using the Trebouxiophycean alga Coccomyxa subellipsoidea grown in a chemostatic bioreactor system. The data provides further evidence that growth and triglyceride (TAG) accumulation are concurrent and independently proportional to the degree of nitrogen limitation in algae. Additionally, TAG accumulation was observed to proceed via oscillations with respect to time and percent dry weight quantity. The predator-prey model was applied to fit the experimental data and to obtain the physiological significance of these oscillations. The results determine the conditions of maximum neutral lipid productivity with respect to nitrate stress and highlight an area of potential future research.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751 · see citing works
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/y59-099 · Cited by 23,189 · see citing works
Brennan, L., & Owende, P. (2010) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2009.10.009 · Cited by 4,894 · see citing works
Microalgae as a raw material for biofuels production
Gouveia, L., & Oliveira, A. C. (2009) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-008-0495-6 · Cited by 1,284 · see citing works
A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae
Chen, W., Zhang, C., Song, L., Sommerfeld, M., & Hu, Q. (2009) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2009.01.001 · Cited by 681 · see citing works
Breuer, G., Lamers, P. P., Martens, D. E., Draaisma, R. B., & Wijffels, R. H. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.08.003 · Cited by 626 · see citing works
Computational approaches to cellular rhythms
Goldbeter, A. (2002) · Nature · doi:10.1038/nature01259 · Cited by 581 · see citing works
The Morphology of Chlamydomonas chlamydogama, Sp. Nov.
Bold, H. C. (1949) · Bulletin of the Torrey Botanical Club · doi:10.2307/2482218 · Cited by 494 · see citing works
Trentacoste, E. M., Shrestha, R. P., Smith, S. R., Glé, C., Hartmann, A. C., Hildebrand, M., & Gerwick, W. H. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1309299110 · Cited by 430 · see citing works
Adams, C., Godfrey, V., Wahlen, B., Seefeldt, L., & Bugbee, B. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2012.12.143 · Cited by 242 · see citing works
Devi, M. P., Subhash, G. V., & Mohan, S. V. (2011) · Renewable Energy · doi:10.1016/j.renene.2011.11.021 · Cited by 228 · see citing works
Simultaneous growth and neutral lipid accumulation in microalgae
Klok, A. J., Martens, D. E., Wijffels, R. H., & Lamers, P. P. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.02.006 · Cited by 213 · see citing works
Energy from algae: Current status and future trends
Yusuf Chisti, B., & Yan, J. (2011) · Applied Energy · doi:10.1016/j.apenergy.2011.04.038 · Cited by 210 · see citing works
Yeh, K.-L., & Chang, J. (2011) · Biotechnology Journal · doi:10.1002/biot.201000433 · Cited by 205 · see citing works
Richardson, B., Orcutt, D. M., Schwertner, H. A., Martinez, C. L., & Wickline, H. E. (1969) · Applied Microbiology · doi:10.1128/am.18.2.245-250.1969 · Cited by 153 · see citing works
Two phase microalgae growth in the open system for enhanced lipid productivity
Das, P., Aziz, S. S., & Obbard, J. P. (2011) · Renewable Energy · doi:10.1016/j.renene.2011.02.002 · Cited by 144 · see citing works
Hoffmann, M., Marxen, K., Schulz, R., & Vanselow, K. (2010) · Marine Drugs · doi:10.3390/md8092526 · Cited by 118 · see citing works
Hildebrand, M., & Dahlin, K. (2000) · Journal of Phycology · doi:10.1046/j.1529-8817.2000.99153.x · Cited by 111 · see citing works
Mitsutoshi Fujita, A. (2014) · Elsevier eBooks · doi:10.1016/c2012-0-00459-0 · Cited by 110 · see citing works
Tevatia, R., Demi̇rel, Y., & Blum, P. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.05.124 · Cited by 56 · see citing works
Self-citationLacour, T., Sciandra, A., Talec, A., Mayzaud, P., & Olivier Bernard, A. (2012) · Journal of Phycology · doi:10.1111/j.1529-8817.2012.01154.x · Cited by 53 · see citing works
Allen, J. W., Dirusso, C., & Black, P. N. (2014) · Algal Research · doi:10.1016/j.algal.2014.04.003 · Cited by 17 · see citing works
Self-citationSmouse, P. E. (1981) · Theoretical Population Biology · doi:10.1016/0040-5809(81)90007-1 · Cited by 8 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae
Maltsev, Y., Kulikovskiy, M., & Maltseva, S. (2023) · Microbial Cell Factories · doi:10.1186/s12934-023-02244-6 · Cited by 174 · see citing works
“Recent studies support the ability to regulate micro-algae growth and lipid accumulation through precision nutrient restriction [43].”
BackgroundZhang, T., Li, S., Chen, L., Zhang, W., & Sun, T. (2024) · ACS Sustainable Chemistry & Engineering · doi:10.1021/acssuschemeng.4c04497 · Cited by 6 · see citing works
- Authors
- Tevatia, R., Allen, J., Blum, P., Demirel, Y., & Black, P.
- Affiliations
- University of Nebraska–Lincoln (US)
- Corresponding author
- Tevatia, Rahul
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2014-10
- Volume
- 170
- Pages
- 152-159
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2068333195
- Cited by
- 29
- References
- 23
- Citations by year
- 2025: 4 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 2 · 2015: 5
- FWCI
- 1.10
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Bioresource Technology (acceptedVersion)
- PubMed (publishedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- Department of Energy (DE-FG36-08GO88055)
- National Science Foundation (EPSCOR)
- Office of Research and Economic Development, University of Nebraska, Lincoln, NE
- University of Nebraska-Lincoln Cell Development Facility
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- nitrogen limitation, triglyceride accumulation, rhythmic oscillations, predator-prey model, green microalgae, chemostat, nitrate stress, lipid productivity, mutual growth
- MeSH terms
- Chlorophyta, Chromatography, Liquid, Models, Biological, Nitrogen, Periodicity, Triglycerides, Biomass, Bioreactors, Tandem Mass Spectrometry, Microalgae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
