Optimization of cell disruption methods for efficient recovery of bioactive metabolites via NMR of three freshwater microalgae (chlorophyta)
Abstract
This study demonstrates the use of NMR techniques coupled with chemometric analysis as a high throughput data mining method to identify and examine the efficiency of different disruption techniques tested on microalgae (Chlorella variabilis, Scenedesmus regularis and Ankistrodesmus gracilis). The yield and chemical diversity from the disruptions together with the effects of pre-oven and pre-freeze drying prior to disruption techniques were discussed. HCl extraction showed the highest recovery of oil compounds from the disrupted microalgae (up to 90%). In contrast, NMR analysis showed the highest intensity of bioactive metabolites obtained for homogenized extracts pre-treated with freeze-drying, indicating that homogenizing is a more favorable approach to recover bioactive substances from the disrupted microalgae. The results show the potential of NMR as a useful metabolic fingerprinting tool for assessing compound diversity in complex microalgae extracts.
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Ma et al. optimized a microalgae cell disruption technique using nuclear magnetic resonance (NMR) coupled with chemometrics.
“Ma et al. [108] optimized a microalgae cell disruption technique using nuclear magnetic resonance (NMR) coupled with chemometrics.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [108]
Recent studies in microalgae employ extraction strategies that involve the selection of improved formula for solvents, as well as other technologies like supercritical fluid extraction and electrical disruption, which do not use toxic solvents.
“Recent studies in microalgae employ extraction strategies that involve the selection of improved formula for solvents, as well as other technologies like supercritical fluid extraction and electrical disruption, which do not use toxic solvents [108].”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [108]
In Ma et al.'s study, identification of metabolites from a high-complexity microalgae extract was achieved.
“The identification of metabolites from a high complexity of microalgae extract was achieved in this study.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [108]
In Ma et al.'s study, PCA and PLSDA revealed the effect of pre-treatment.
“Furthermore, the PCA and partial list squares discriminant analysis (PLSDA) revealed the effect of pre-treatment.”
From Factors Affecting Microalgae Production for Biofuels and the Potentials of Chemometric Methods in Assessing and Optimizing Productivity (2019) · cites this paper as [108]
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- Authors
- Ma, N. L., Teh, K. Y., Lam, S. S., Kaben, A. M., & Cha, T. S.
- Affiliations
- Universiti Malaysia Terengganu (MY)
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2015-08
- Volume
- 190
- Pages
- 536-542
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2063366506
- Cited by
- 19
- References
- 28
- Citations by year
- 2026: 2 · 2025: 3 · 2023: 1 · 2021: 2 · 2020: 5 · 2019: 3 · 2017: 2 · 2016: 1
- FWCI
- 0.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Universiti Malaysia Terengganu (59264)
- Ministry of Higher Education, Malaysia
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- cell disruption, microalgae, Chlorella variabilis, bioactive metabolites, NMR metabolomics, homogenization, sonication, HCl extraction, freeze-drying, metabolic fingerprinting, chemometrics
- MeSH terms
- Chlorophyta, Biological Factors, Cell Fractionation, Freezing, Hydrochloric Acid, Magnetic Resonance Spectroscopy, Sonication
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