The DOI matches its Crossref record; 26 of the 27 references it cites were found in a registry or an index.
Production, extraction and stabilization of lutein from microalga Chlorella sorokiniana MB-1
Abstract
The efficiencies of extraction and preservation of lutein from microalgae are critical for the success of its commercialization. In this study, lutein was produced by Chlorella sorokiniana MB-1 via semi-batch mixotrophic cultivation. The microalgal biomass with a lutein content of 5.21mg/g was pretreated by bead-beating and high pressure cell disruption methods, and the lutein content was harvested by a reduced pressure extraction method. The effect of pretreatment, pressure, solvent type, extraction time and temperature on lutein recovery was investigated. Using high pressure pretreatment followed by extraction with tetrahydrofuran (THF) as solvent resulted in high lutein recovery efficiencies of 87.0% (20min) and 99.5% (40min) at 850mbar and 25°C. In contrast, using ethanol as the solvent, 86.2% lutein recovery was achieved under 450mbar, 35°C and 40min extraction. The extracted lutein was stabilized in olive oil or sunflower oil with half-lives of 53.1 and 63.8days, respectively.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“For instance, Chen et al. (2016a, b) reported that the use of high pressure followed by extraction with tetrahydrofuran could result in 99.5% lutein recovery from C. sorokiniana (Chen et al. 2016a, b).”
From Production of microalgae with high lipid content and their potential as sources of nutraceuticals (2023) · cites this paper as Chen et al. 2016a, b
Citations
Cites
Recovery of microalgal biomass and metabolites: Process options and economics
Molina Grima, E., Belarbi, E.-H., Acién Fernández, F. G., Robles Medina, A., & Chisti, Y. (2003) · Biotechnology Advances · doi:10.1016/s0734-9750(02)00050-2 · Cited by 2,286
Jeffrey, S. W., Mantoura, R. F. C., & Wright, S. W. (2000) · Time to knit · doi:10.1016/s0967-0653(97)89288-5 · Cited by 1,265
Antioxidants in Photosynthesis and Human Nutrition
Demmig‐Adams, B., & Adams, W. W. (2002) · Science · doi:10.1126/science.1078002 · Cited by 685
Vitamin E and its function in membranes
Wang, X. (1999) · Progress in Lipid Research · doi:10.1016/s0163-7827(99)00008-9 · Cited by 560
Overview of Mechanisms of Action of Lycopene
Heber, D., & Lu, Q. (2002) · Experimental Biology and Medicine · doi:10.1177/153537020222701013 · Cited by 478
Microalgal cell disruption for biofuel development
Halim, R., Harun, R., Danquah, M. K., & Webley, P. A. (2011) · Applied Energy · doi:10.1016/j.apenergy.2011.08.048 · Cited by 347
Oxygenated Carotenoid Lutein and Progression of Early Atherosclerosis
Dwyer, J. H., Navab, M., Dwyer, K. M., Hassan, K., Sun, P., Shircore, A. M., Hama-Levy, S., Hough, G., Wang, X., Drake, T. A., et al. (2001) · Circulation · doi:10.1161/01.cir.103.24.2922 · Cited by 338
Relative solubility, stability, and absorptivity of lutein and .beta.-carotene in organic solvents
Craft, N. E., & Soares, J. H. (1992) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf00015a013 · Cited by 311
Carotenoid–radical interactions
Krinsky, N. I., & Yeum, K. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(03)00816-7 · Cited by 268
Food carotenoids and cancer prevention: An overview of current research
Astorg, P. (1997) · Trends in Food Science & Technology · doi:10.1016/s0924-2244(97)01092-3 · Cited by 234
Flagged first, then by citations
1 of the 27 references couldn’t be matched to a known paper.
Research works printed but not linked: 1
- Mantoura, 1997, Phytoplankton pigments in oceanography: guidelines to modern methods, Oceanogr. Literature Rev., 10, 1110
Cited by
Sorted by citations
Items 101–116 of 116
Bildik, F. (2026) · ACS Omega · doi:10.1021/acsomega.5c09347 · Cited by 1
Thakur, B., Kant, A., Dhar, H., & Nadda, A. K. (2026) · Process Biochemistry · doi:10.1016/j.procbio.2026.05.019 · Cited by 1
Selvi, M. J. T., Rani, V., Padmavathy, P., Amirtharaj, K. S. V., Leema, J. T. M., Vennila, M., & Ramesh, B. (2023) · Regional Studies in Marine Science · doi:10.1016/j.rsma.2023.103291 · Cited by 1
Continuous microalgal culture module and method of culturing microalgae containing macular pigment
Lin, M., Lin, C., Chen, Y.-T., Huang, S.-Q., Yang, Y.-C., Zhang, W., Chiu, W.-H., Lin, C., & Kuo, C.-M. (2024) · Bioresource Technology · doi:10.1016/j.biortech.2024.130714 · Cited by 1
Ties That Free: A Literature Cluster Exploring Relationships That Empower.
Kieff, J. (2002) · Journal of children's literature · Cited by 1
Gong, M. (2017) · Scholarship@Western (Western University)
Tunyasitikun, P., & Shotipruk, A. (2016) · doi:10.58837/chula.the.2016.1383
Algae and cyanobacteria biorefinery
Dahunsi, O. S. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-24129-1.00019-3
Plant and Algae-Based Biocolors
Singh, G., Bose, P., Kamakshi, Rani, R., & Kakkar, P. M. (2025) · Springer eBooks · doi:10.1007/978-981-95-1738-1_4
Downstream Processing of the Microalgal Pigments: A Close Look at Stability
Maroneze, M. M., Morales‐Sánchez, D., & Martinez, A. (2026) · Springer eBooks · doi:10.1007/978-3-032-05438-8_4
Microalgal Carotenoids in Heterotrophic Cultures
Camarena-Bernard, C., & Pozzobon, V. (2026) · Springer eBooks · doi:10.1007/978-3-031-93945-7_4
Camarena-Bernard, C., Salas, D. L., Theo, J. A. B., Balti, R., & Pozzobon, V. (2026) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-026-03319-5
Extraction of biomolecules from microalgae
Hu, Y., & Bassi, A. S. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-34082-6.00012-x
Camarena-Bernard, C. (2024) · theses.fr (ABES) · doi:10.70675/2fa6f2a9z3e6cz496eza4b0z240cc01b75e8
Sebahi, R., Verret, F., Mandalakis, M., & Hamed, M. B. B. (2026) · Applied Phycology · doi:10.1080/26388081.2026.2671068
Muhammad, G., Yuan, X., Li, Z., Zhang, X., & Zhang, Z. (2026) · Results in Chemistry · doi:10.1016/j.rechem.2026.103861
Metadata
- Authors
- Chen, C.-Y., Jesisca, Hsieh, C., Lee, D.-J., Chang, C.-H., & Chang, J.-S.
- Affiliations
- National Cheng Kung University (TW)
- National Kaohsiung Normal University (TW)
- National Taiwan University (TW)
- Corresponding author
- Chang, Jo-Shu
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2016-01
- Volume
- 200
- Pages
- 500-505
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2192216707
- Cited by
- 116
- References
- 27
- Citations by year
- 2026: 8 · 2025: 8 · 2024: 12 · 2023: 10 · 2022: 17 · 2021: 14 · 2020: 13 · 2019: 11 · 2018: 7 · 2017: 10 · 2016: 5
- FWCI
- 2.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education
- Ministry of Science and Technology, Taiwan (103-2221-E-006-190-MY3, MOST104-3113-E-006-003)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Keywords
- lutein, Chlorella sorokiniana, microalgae, mixotrophic cultivation, bead beating, lutein extraction, tetrahydrofuran, ethanol extraction, olive oil, sunflower oil, carotenoid recovery
- MeSH terms
- Olive Oil, Sunflower Oil, Chlorella, Furans, Plant Oils, Pressure, Solvents, Temperature, Lutein, Biomass, Microalgae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
