Lipid accumulation of Chlorella pyrenoidosa under mixotrophic cultivation using acetate and ammonium
Abstract
Acetate and ammonium were used as organic carbon and nitrogen sources, respectively, during mixotrophic cultivation of Chlorella pyrenoidosa. Cell growth, content of neutral lipid (NL), productivity of biomass and total lipid, and fatty acid profiles were investigated. Results showed that C. pyrenoidosa could endure high concentrations of NH 4+-N (100-200 mg/L) and immediately entered logarithmic growth, when the culture media contained 2.0-10.0 g/L NaAc. The 2.0-10.0 g/L NaAc in the media also resulted in the NL content of 1.87-3.05 mg/10 9 cells, much higher than 0.5 mg/10 9 cells of the controls. The maximum productivities of biomass and total lipid were achieved under 50 and 10 mg/L NH 4+-N respectively when the 2.0 g/L NaAc was dosed. The fatty acids were mainly composed of C16:0, C16:1, C18:0, and C18:1 under the mixotrophic cultivation, with the higher saturation compared to the controls.
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For mixotrophic cultivation of C. pyrenoidosa, increasing acetate concentration up to 10 g/L improved neutral lipid content and increased concentrations of saturated (C16:0, C18:0) and monosaturated (C16:1, C18:1) fatty acids.
“An improvement of the neutral lipids content and higher concentrations of saturated (e.g., C16:0 and C18:0) and monosaturated fatty acids (e.g., C16:1 and C18:1), was also observed for mixotrophic cultivation of C. pyrenoidosa when acetate concentration was increased up to 10 g L -1 [45].”
From Challenges and Opportunities to Increase Economic Feasibility and Sustainability of Mixotrophic Cultivation of Green Microalgae of the Genus Chlorella (2022) · cites this paper as [45]
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- Authors
- Liu, L., Zhao, Y., Jiang, X., Wang, X., & Liang, W.
- Affiliations
- Beijing Forestry University (CN)
- Corresponding author
- Liang, Wenyan
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2018-08
- Volume
- 262
- Pages
- 342-346
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2801753186
- Cited by
- 56
- References
- 19
- Citations by year
- 2026: 2 · 2025: 9 · 2024: 6 · 2023: 2 · 2022: 14 · 2021: 10 · 2020: 4 · 2019: 5 · 2018: 3
- FWCI
- 1.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (51672028)
- Fundamental Research Funds for the Central Universities (2015ZCQ-HJ-02)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- Chlorella pyrenoidosa, mixotrophic cultivation, lipid accumulation, acetate, ammonium, fatty acid composition, biomass productivity, nitrogen source, microalgae
- MeSH terms
- Acetates, Chlorella, Lipids, Biomass, Lipid Metabolism, Microalgae, Ammonium Compounds
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