Cited by 56Algal biology and biofuel productionAquaculture Nutrition and GrowthMicrobial Metabolic Engineering and Bioproduction

Lipid accumulation of Chlorella pyrenoidosa under mixotrophic cultivation using acetate and ammonium

Liu, Lijun, Zhao, Yuan, Jiang, Xiaoxue, Wang, Xiaoyu, Liang, Wenyan·2018·Bioresource Technology

Abstract

Acetate and ammonium were used as organic carbon and nitrogen sources, respectively, during mixotrophic cultivation of Chlorella pyrenoidosa. Cell growth, content of neutral lipid (NL), productivity of biomass and total lipid, and fatty acid profiles were investigated. Results showed that C. pyrenoidosa could endure high concentrations of NH 4+-N (100-200 mg/L) and immediately entered logarithmic growth, when the culture media contained 2.0-10.0 g/L NaAc. The 2.0-10.0 g/L NaAc in the media also resulted in the NL content of 1.87-3.05 mg/10 9 cells, much higher than 0.5 mg/10 9 cells of the controls. The maximum productivities of biomass and total lipid were achieved under 50 and 10 mg/L NH 4+-N respectively when the 2.0 g/L NaAc was dosed. The fatty acids were mainly composed of C16:0, C16:1, C18:0, and C18:1 under the mixotrophic cultivation, with the higher saturation compared to the controls.

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Crossref · 10.1016/j.biortech.2018.04.092

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Authors
Liu, L., Zhao, Y., Jiang, X., Wang, X., & Liang, W.
Affiliations
Beijing Forestry University (CN)
Corresponding author
Liang, Wenyan
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2018-08
Volume
262
Pages
342-346
ISSN
0960-8524 (ISSN-L)
OpenAlex
W2801753186
Cited by
56
References
19
Citations by year
2026: 2 · 2025: 9 · 2024: 6 · 2023: 2 · 2022: 14 · 2021: 10 · 2020: 4 · 2019: 5 · 2018: 3
FWCI
1.27
Type
journal article
Language
en
Open access
closed
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (51672028)
  • Fundamental Research Funds for the Central Universities (2015ZCQ-HJ-02)
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords
Chlorella pyrenoidosa, mixotrophic cultivation, lipid accumulation, acetate, ammonium, fatty acid composition, biomass productivity, nitrogen source, microalgae
MeSH terms
Acetates, Chlorella, Lipids, Biomass, Lipid Metabolism, Microalgae, Ammonium Compounds

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