Cited by 47Algal biology and biofuel productionAquaculture Nutrition and GrowthAquatic Ecosystems and Phytoplankton Dynamics

Optimizing heterotrophic production of Chlorella sorokiniana SU-9 proteins potentially used as a sustainable protein substitute in aquafeed

Chen, Chun-Yen, Lu, Jhih-Ci, Chang, Yu-Han, Chen, Jih-Heng, Nagarajan, Dillirani, Lee, Duu-Jong, Chang, Jo-Shu·2023·Bioresource Technology

Abstract

Alternative protein sources for the reduction/replacement of fish meal in aqua-feeds are in urgent demand. Microalgae are considered sustainable protein sources for aquaculture due to their high-quality proteins with a complete profile of essential amino acids. This study examined the heterotrophic production of proteins from Chlorella sorokiniana SU-9. Culture parameters for maximal biomass and protein production are as follows: glucose - 10 g/L glucose, sodium nitrate - 1.5 g/L, and iron - 46 μM iron in BG-11 medium. Under optimal conditions, biomass content, protein content and protein productivity of SU-9 reached 4.14 ± 0.20 g/L, 403 ± 33 mg/g and 382 ± 36 mg/L/d, respectively. The protein profile of Chlorella sorokiniana SU-9 is comparable to fishmeal and soybean meal. The essential amino acids arginine, lysine and cysteine, along with glutamine and glutamate, were high. The production cost of SU-9 can be significantly reduced under heterotrophic cultivation conditions, making it a potential protein substitute in aquafeed.

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Crossref · 10.1016/j.biortech.2022.128538

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Authors
Chen, C.-Y., Lu, J.-C., Chang, Y.-H., Chen, J.-H., Nagarajan, D., Lee, D.-J., & Chang, J.-S.
Affiliations
  • National Cheng Kung University (TW)
  • Tunghai University (TW)
  • National Taiwan University (TW)
  • City University of Hong Kong (HK)
Corresponding author
  • Chang, Yu-Han
  • Chang, Jo-Shu
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2023-02
Volume
370
Pages
128538
ISSN
0960-8524 (ISSN-L)
OpenAlex
W4312222069
Cited by
47
References
44
Citations by year
2026: 13 · 2025: 16 · 2024: 11 · 2023: 7
FWCI
1.53
Type
journal article
Language
en
Open access
closed
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministry of Science and Technology, Taiwan
Topics
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
  • Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
Keywords
heterotrophic cultivation, microalgal proteins, aquaculture feed, fish meal replacement, essential amino acids, culture parameter optimization, biomass production, protein productivity, production costs, sustainable protein sources
MeSH terms
Amino Acids, Essential, Animals, Chlorella, Glucose, Biomass, Heterotrophic Processes, Microalgae

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