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Dopamine biosensor based on surface functionalized nanostructured nickel oxide platform
Abstract
A dopamine biosensor has been developed using nickel oxide nanoparticles (NPs) and tyrosinase enzyme conjugate. Nickel oxide (NiO) NPs were synthesized by sol-gel method using anionic surfactant, sodium dodecyl sulphate (SDS), as template to control the size of synthesized nanoparticles. The structural and morphological studies of the prepared NPs were carried out using X-ray diffraction (XRD), transmission electron microscopy (TEM) and dynamic light scattering (DLS) techniques. Afterwards, tyrosinase enzyme molecules were adsorbed on NiO NPs surface and enzyme coated NPs were deposited on indium tin oxide (ITO) coated flexible polyethylene terephthalate (PET) substrate by solution casting method. The formation of enzyme-NPs conjugate was investigated by atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR) techniques and used in selective detection and estimation of neurochemical dopamine by electrochemical method. The fabricated Tyrosinase/NiO/ITO electrode exhibits high sensitivity of 60.2nA/µM in linear detection range (2-100μM) with a detection limit of 1.038μM. The proposed sensor had a response time of 45s, long shelf life (45 days) with good reproducibility and selectivity in presence of interfering substances and was validated with real samples. The tyrosinase enzyme functionalized NiO platform has good bio-sensing efficacy and can be used in detection of other catecholamines and phenolic neurochemicals.
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“Prior work using biosensors to measure stress and cognition has focused on the measurement of a wide array of biomarkers (10−6–10−12 M) such as Orexin-A,113 cortisol,110 dopamine,114 neuropeptide Y,115 and interleukin-6 (IL-6).116”
From Wearable sensors for monitoring the physiological and biochemical profile of the athlete (2019) · cites this paper as [114]
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Metadata
- Authors
- Roychoudhury, A., Basu, S., & Jha, S. K.
- Affiliations
- Indian Institute of Technology Delhi (IN)
- Corresponding author
- Jha, Sandeep Kumar
- Venue
- Biosensors and Bioelectronics
- Publisher
- Elsevier BV
- Published
- 2016-10
- Volume
- 84
- Pages
- 72-81
- ISSN
- 0956-5663 (ISSN-L)
- OpenAlex
- W2209936051
- Cited by
- 184
- References
- 67
- Citations by year
- 2026: 7 · 2025: 18 · 2024: 17 · 2023: 19 · 2022: 14 · 2021: 11 · 2020: 23 · 2019: 26 · 2018: 16 · 2017: 26 · 2016: 7
- FWCI
- 7.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biosensors and Bioelectronics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Department of Biotechnology, Government of India (BT/PR7190/PID/6/719/2012)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Electrochemical sensors and biosensors
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- dopamine biosensor, nickel oxide nanoparticles, tyrosinase, electrochemical biosensor, sodium dodecyl sulfate, flexible PET substrate, indium tin oxide electrode, catecholamine detection, sol-gel synthesis
- MeSH terms
- Agaricus, Animals, Cattle, Dopamine, Enzymes, Immobilized, Nickel, Polyethylene Terephthalates, Monophenol Monooxygenase, Biosensing Techniques, Tin Compounds, Nanoparticles, Electrochemical Techniques, Limit of Detection
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