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Cited by 6Electrochemical sensors and biosensorsElectrochemical Analysis and ApplicationsAnalytical Chemistry and Sensors

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Ligand discrimination in hOR1A1 based on the capacitive response

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Lagunas, Anna, Belloir, Christine, Lalis, Maxence, Briand, Loïc, Topin, Jérémie, Gorostiza, Pau, Samitier, Josep·2025·Biosensors and Bioelectronics
DOI 10.1016/j.bios.2024.117000

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Abstract

Odorant discrimination mechanisms are based on the differential interactions between odorant molecules and olfactory receptors (ORs). Biohybrid sensors based on ORs described to date show selectivity towards specific versus non-specific binding of odorants, being unable to distinguish between specific ligands of different affinity. Here we disclose a method that enables odorant discrimination based on the modulation of the capacitive response of the receptor, which allows the differentiation of three high-affinity hOR1A1 agonists. We performed voltammetry and impedance measurements of the hOR1A1 receptor selectively immobilized on a gold electrode in the absence and presence of the agonists. Binding induces a decrease in the capacitive response of the receptor that is proportional to the ligand potency, reaching up to a 40% decrease for the cognate ligand dihydrojasmone, which is attributed to changes in the magnitude and orientation of the electric dipole in the receptor, thereby regulating its response to the applied electric field.

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Metadata

Authors
Lagunas, A., Belloir, C., Lalis, M., Briand, L., Topin, J., Gorostiza, P., & Samitier, J.
Affiliations
  • Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (ES)
  • Centre National de la Recherche Scientifique (FR)
  • Université Côte d'Azur (FR)
  • Institució Catalana de Recerca i Estudis Avançats (ES)
  • Instituto de Salud Carlos III (ES)
Corresponding author
Lagunas, Anna
ORCID
Venue
Biosensors and Bioelectronics
Publisher
Elsevier BV
Published
2025-03
Volume
271
Pages
117000
ISSN
0956-5663 (ISSN-L)
DOI
10.1016/j.bios.2024.117000
OpenAlex
W4404907120
Cited by
6
References
1
Citations by year
2026: 3 · 2025: 3
FWCI
0.62
Type
journal article
Language
en
Open access
Licence
Locations
  • Biosensors and Bioelectronics (publishedVersion)
  • SSRN Electronic Journal (acceptedVersion)
  • ChemRxiv (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by-nc-nd)
  • HAL (submittedVersion)
Funders
  • Centres de Recerca de Catalunya
  • Agence Nationale de la Recherche (ANR-18-CE30-0006)
  • Generalitat de Catalunya (2021SGR-01545)
  • Fondation de France
  • Instituto de Salud Carlos III
  • European Regional Development Fund
Topics
  • Physical Sciences › Chemistry › Analytical Chemistry › Electrochemical sensors and biosensors
  • Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
  • Physical Sciences › Chemistry › Analytical Chemistry › Analytical Chemistry and Sensors
Keywords
olfactory receptors, odor discrimination, ligand discrimination, capacitive response, gold electrode, dihydrojasmone, electric dipole, receptor immobilization, impedance sensing, voltammetry, ligand potency
MeSH terms
Electrodes, Gold, Humans, Ligands, Odorants, Biosensing Techniques, Receptors, Odorant, Electric Capacitance, Electrochemical Techniques

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