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Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases
Open accessAbstract
The development of customizable sequence-specific nucleases such as TALENs, ZFNs and the powerful CRISPR/Cas9 system has revolutionized the field of genome editing. The CRISPR/Cas9 system is particularly versatile and has been applied in numerous species representing all branches of life. Regardless of the target organism, all researchers using sequence-specific nucleases face similar challenges: confirmation of the desired on-target mutation and the detection of off-target events. Here, we evaluate the most widely-used methods for the detection of on-target and off-target mutations in terms of workflow, sensitivity, strengths and weaknesses.
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“| Delivery methods improvement | Application in vivo [95–105] | LNP: Can be quickly degraded in vivo | Eliminate off-target effects |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [103]
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In the citing paper
“| Delivery methods improvement | Application in vivo [95–105] | AAV: Last for years in terminally differentiated cell types | May cause accumulation of off-target mutations |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [103]
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“When the indels are deposited at the coding sequences of a given gene, NHEJ can cause frameshift mutations, resulting in non-sense-mediated mRNA decay and gene silencing (Wang et al., 2016a; Zischewski et al., 2017; Lindeboom et al., 2019).”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as Zischewski et al., 2017
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“| Delivery methods improvement | Application in vivo [95–105] | LNP: Can be quickly degraded in vivo | Eliminate off-target effects |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [103]
Experimental — accuracy not yet measuredThe check wasn’t confident either way, so it gives no verdict — never disputed.
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“| Delivery methods improvement | Application in vivo [95–105] | AAV: Last for years in terminally differentiated cell types | May cause accumulation of off-target mutations |”
From Off-target effects in CRISPR/Cas9 gene editing (2023) · cites this paper as [103]
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Cites
A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116
DNA targeting specificity of RNA-guided Cas9 nucleases
Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741
Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9
Doench, J. G., Fusi, N., Sullender, M., Hegde, M., Vaimberg, E. W., Donovan, K. F., Smith, I., Tothova, Z., Wilen, C., Orchard, R., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3437 · Cited by 4,637
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
Ran, F. A., Hsu, P. D., Lin, C.-Y., Gootenberg, J. S., Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., et al. (2013) · Cell · doi:10.1016/j.cell.2013.08.021 · Cited by 3,299
High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells
Fu, Y., Foden, J. A., Khayter, C., Maeder, M. L., Reyon, D., Joung, J. K., & Sander, J. D. (2013) · Nature Biotechnology · doi:10.1038/nbt.2623 · Cited by 3,292
CRISPR-Cas systems for editing, regulating and targeting genomes
Sander, J. D., & Joung, J. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2842 · Cited by 3,153
In vivo genome editing using Staphylococcus aureus Cas9
Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., et al. (2015) · Nature · doi:10.1038/nature14299 · Cited by 2,687
High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects
Kleinstiver, B. P., Pattanayak, V., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Zheng, Z., & Joung, J. K. (2016) · Nature · doi:10.1038/nature16526 · Cited by 2,673
RNA-guided editing of bacterial genomes using CRISPR-Cas systems
Jiang, W., Bikard, D., Cox, D., Zhang, F., & Marraffini, L. A. (2013) · Nature Biotechnology · doi:10.1038/nbt.2508 · Cited by 2,429
Rationally engineered Cas9 nucleases with improved specificity
Slaymaker, I. M., Gao, L., Zetsche, B., Scott, D. A., Yan, W. X., & Zhang, F. (2016) · Science · doi:10.1126/science.aad5227 · Cited by 2,414
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Sessions, K. J., Chen, Y. Y., Hodge, C. A., Hudson, T. R., Eszterhas, S. K., Hayden, M. S., & Howell, A. L. (2020) · AIDS Research and Human Retroviruses · doi:10.1089/aid.2020.0055 · Cited by 8
Mascarenhas, M. S., Nascimento, F. D. S., Rocha, A. D. J., Ferreira, M. D. S., Oliveira, W. D. D. S., Lino, L. S. M., Mendes, T. A. D. O., Ferreira, C. F., dos Santos-Serejo, J. A., & Amorim, É. P. (2024) · Current Issues in Molecular Biology · doi:10.3390/cimb46100659 · Cited by 9
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Li, Q., & Lin, X. (2026) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2026.118704 · Cited by 9
Ijaz, F., Nakazato, R., Setou, M., & Ikegami, K. (2022) · Scientific Reports · doi:10.1038/s41598-022-15776-5 · Cited by 7
Drapal, M., Enfissi, E. M. A., Almeida, J. C., Rapacz, E., Nogueira, M., & Fraser, P. D. (2023) · Transgenic Research · doi:10.1007/s11248-023-00347-9 · Cited by 7
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Cho, G. Y., Bolo, K. A., Park, K. S., Sengillo, J. D., & Tsang, S. H. (2018) · Molecular Diagnosis & Therapy · doi:10.1007/s40291-018-0377-1 · Cited by 8
Modulating Cas9 activity for precision gene editing
Uslu, M., Si̇yah, P., Harvey, A. J., & Kocabaş, F. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2021.01.015 · Cited by 7
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Xu, C. L., Ruan, M. Z., Ragi, S. D., & Tsang, S. H. (2023) · Methods in molecular biology · doi:10.1007/978-1-0716-2651-1_26 · Cited by 6
Zanatta, C. B., Narendja, F. M., Jawhary, H. E., Sleymane, G. A., Subburaj, S., Nodari, R. O., & Agapito-Tenfen, S. Z. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26073308 · Cited by 6
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Application and Development of CRISPR/Cas9 Technology in Pig Research
Lin, H., Deng, Q., Li, L., & Shi, L. (2019) · IntechOpen eBooks · doi:10.5772/intechopen.85540 · Cited by 6
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Zanatta, C. B., Hoepers, A. M., Nodari, R. O., & Agapito-Tenfen, S. Z. (2023) · Foods · doi:10.3390/foods12234298 · Cited by 6
Luttgeharm, K. D., Wong, K., & Siembieda, S. (2017) · BioTechniques · doi:10.2144/000114556 · Cited by 6
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Soni, S., & Kohn, D. B. (2019) · Cytotherapy · doi:10.1016/j.jcyt.2018.12.001 · Cited by 6
Human embryo gene editing: God's scalpel or Pandora's box?
Zhou, Q., Zhang, Y., Zou, Y., Yin, T., & Yang, J. (2019) · Briefings in Functional Genomics · doi:10.1093/bfgp/elz025 · Cited by 6
Orlova, N. A., Sinegubova, M. V., Kolesov, D., Khodak, Y. A., Tatarskiy, V. V., & Vorobiev, I. I. (2025) · Cells · doi:10.3390/cells14100692 · Cited by 5
Altgilbers, S., Dierks, C., Klein, S., Weigend, S., & Kues, W. A. (2022) · Scientific Reports · doi:10.1038/s41598-022-19861-7 · Cited by 5
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Hong, J., Shi, Q., Biswas, S., Jiang, S., & Shi, J. (2020) · Food Control · doi:10.1016/j.foodcont.2020.107790 · Cited by 5
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Guichard, S. M. (2017) · Annual reports in medicinal chemistry · doi:10.1016/bs.armc.2017.08.006 · Cited by 4
Liu, X., Qiu, S., Mei, L., Jing, H., Lin, X., & Wang, Q. (2020) · Journal of AOAC International · doi:10.1093/jaoacint/qsaa161 · Cited by 4
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Gong, J. D., Samarkhazan, H. S., Siavashi, M., Servatian, N., & Pirsavabi, F. (2025) · Molecular Biology Reports · doi:10.1007/s11033-025-11235-2 · Cited by 5
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Chang, X., & Hou, Y. (2018) · Europe PMC (PubMed Central) · Cited by 3
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Mac, T. T., Fauquier, T., Jullien, N., Romanet, P., Etchevers, H., Barlier, A., Castinetti, F., & Brue, T. C. (2023) · eLife · doi:10.7554/elife.90875.3 · Cited by 14
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Wolter, F., Huang, T., & Puchta, H. (2020) · Springer protocols handbooks/Springer protocols · doi:10.1007/978-1-0716-0616-2_2 · Cited by 1
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Jin, Y. (2018) · Stirling Online Research Repository (University of Stirling) · Cited by 1
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Metadata
- Authors
- Zischewski, J., Fischer, R., & Bortesi, L.
- Affiliations
- RWTH Aachen University (DE)
- Fraunhofer Institute for Molecular Biology and Applied Ecology (DE)
- Corresponding author
- Bortesi, Luisa
- Venue
- Biotechnology Advances
- Publisher
- Elsevier BV
- Published
- 2017-01
- Volume
- 35
- Issue
- 1
- Pages
- 95-104
- ISSN
- 0734-9750 (ISSN-L)
- OpenAlex
- W2565555627
- Cited by
- 323
- References
- 78
- Citations by year
- 2026: 14 · 2025: 29 · 2024: 32 · 2023: 31 · 2022: 35 · 2021: 41 · 2020: 40 · 2019: 46 · 2018: 37 · 2017: 18
- FWCI
- 3.04
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Biotechnology Advances (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Research Publications (Maastricht University) (publishedVersion)
- RWTH Publications (RWTH Aachen) (publishedVersion, other-oa)
- Fraunhofer-Publica (Fraunhofer-Gesellschaft) (submittedVersion)
- Funders
- European Research Council (269110)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- CRISPR/Cas9, off-target mutations, TALEN, zinc finger nucleases, sequence-specific nucleases, genome editing, sensitivity
- MeSH terms
- Gene Editing, Animals, Base Sequence, Cell Line, Deoxyribonucleases, Humans, Mutation, Mice, CRISPR-Cas Systems
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