Isoquinoline Alkaloids from Coptis Japonica Stimulate the Myoblast Differentiation Via P38 MAP-Kinase and Akt Signaling Pathway
Abstract
To overcome the muscle atrophy, such as cachexia and sarcopenia, we tried to find myogenic agents from medicinal plants. From myogenic extract of Coptis japonica, we purified six isoquinoline alkaloids and evaluated their effects on transactivation of myoD and MHC expression in C2C12 cells during differentiation process. Among obtained compounds, magnoflorine most efficiently enhanced the myoblast differentiation by activating the p38 MAP kinase and Akt pathway, and also increased the number of multinucleated and cylinder-shaped myotubes. These results propose that magnoflorine from Coptis japonica might be a promising lead compound for the development of anti-muscle atrophy drug.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Isoquinoline alkaloids from Coptis japonica stimulate myoblast differentiation by promoting MyoD transcriptional activity.
“For example, isoquinoline alkaloids from Coptis japonica stimulate myoblast differentiation by promoting MyoD transcriptional activity.”
From Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy (2021) · cites this paper as [132]
Magnoflorine increases expression of MyoD and MHC and stimulates myoblast differentiation via the activation of p38 MAPK and Akt signaling in C2C12 myotubes.
“Magnoflorine increases expression of MyoD and MHC and subsequently stimulates myoblast differentiation via the activation of p38 MAPK and Akt signaling in C2C12 myotubes [132].”
From Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy (2021) · cites this paper as [132]
Cites
p38 MAP-Kinases pathway regulation, function and role in human diseases
Cuenda, A., & Rousseau, S. (2007) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2007.03.010 · Cited by 1,397 · see citing works
Myostatin Inhibits Myoblast Differentiation by Down-regulating MyoD Expression
Langley, B. C., Thomas, M., Bishop, A., Sharma, M., Gilmour, S., & Kambadur, R. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m204291200 · Cited by 910 · see citing works
The circuitry of a master switch: Myod and the regulation of skeletal muscle gene transcription
Tapscott, S. J. (2005) · Development · doi:10.1242/dev.01874 · Cited by 766 · see citing works
p38 and Extracellular Signal-Regulated Kinases Regulate the Myogenic Program at Multiple Steps
Wu, Z.-G., Woodring, P. J., Bhakta, K. S., Tamura, K., Wen, F., Feramisco, J., Karin, M., Wang, J. Y. J., & Puri, P. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.11.3951-3964.2000 · Cited by 524 · see citing works
Activation of an alternative NF‐ΚB pathway in skeletal muscle during disuse atrophy
Hunter, R. B., Stevenson, E. J., Koncarevic, A., Mitchell-Felton, H., Essig, D. A., & Kandarian, S. C. (2002) · The FASEB Journal · doi:10.1096/fj.01-0866com · Cited by 281 · see citing works
Jiang, B., Aoki, M., Zheng, J. Z., Li, J., & Vogt, P. K. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.5.2077 · Cited by 258 · see citing works
Perdiguero, E., Ruiz‐Bonilla, V., Gresh, L., Hui, L., Ballestar, E., Sousa‐Victor, P., Baeza-Raja, B., Jardı́, M., Bosch-Comas, A., Esteller, M., et al. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601587 · Cited by 253 · see citing works
Penn, B. H., Bergstrom, D. A., Dilworth, F. J., Bengal, E., & Tapscott, S. J. (2004) · Genes & Development · doi:10.1101/gad.1234304 · Cited by 184 · see citing works
Skeletal muscle atrophy: Potential therapeutic agents and their mechanisms of action
Dutt, V., Gupta, S. K., Dabur, R., Injeti, E. R., & Mittal, A. (2015) · Pharmacological Research · doi:10.1016/j.phrs.2015.05.010 · Cited by 174 · see citing works
Huang, Z., Han, Z., Ye, B., Dai, Z., Shan, P., Lu, Z., Dai, K., Wang, C., & Huang, W. (2015) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2015.05.028 · Cited by 151 · see citing works
Protein Kinase B β/Akt2 Plays a Specific Role in Muscle Differentiation
Vandromme, M., Rochat, A., Meier, R., Carnac, G., Besser, D., Hemmings, B. A., Fernandez, A., & Lamb, N. J. C. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m005587200 · Cited by 110 · see citing works
Yang, Z., MacQuarrie, K. L., Analau, E., Tyler, A. E., Dilworth, F. J., Cao, Y., Diede, S. J., & Tapscott, S. J. (2009) · Genes & Development · doi:10.1101/gad.1765109 · Cited by 96 · see citing works
Yu, L., Li, F., Zhao, G., Yang, Y., Jin, Z., Zhai, M., Yu, W., Zhao, L., Chen, W., Duan, W., et al. (2015) · APOPTOSIS · doi:10.1007/s10495-015-1122-4 · Cited by 94 · see citing works
Azmi, S., Ozog, A., & Taneja, R. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m409188200 · Cited by 81 · see citing works
Choi, J. S., Ali, M. Y., Jung, H. A., Oh, S. H., Choi, R. J., & Kim, E. J. (2015) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2015.05.020 · Cited by 57 · see citing works
Magnoflorine from Coptidis Rhizoma Protects High Density Lipoprotein during Oxidant Stress
Hưng, T. M., Lee, J., Min, B. S., Choi, J. S., Na, M., Zhang, X., Ngoc, T. M., Lee, I., & Bae, K. (2007) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.30.1157 · Cited by 57 · see citing works
Cdo Interacts with APPL1 and Activates AKT in Myoblast Differentiation
Bae, G., Lee, J.-R., Kim, B.-G., Han, J., Leem, Y.-E., Lee, H.-J., Ho, S., Hahn, M., & Kang, J. (2010) · Molecular Biology of the Cell · doi:10.1091/mbc.e09-12-1011 · Cited by 56 · see citing works
Self-citationIsoquinoline Alkaloids from Tinospora cordifolia Inhibit Rat Lens Aldose Reductase
Patel, M. B., & Mishra, S. (2012) · Phytotherapy Research · doi:10.1002/ptr.3721 · Cited by 55 · see citing works
Cabane, C., Coldefy, A.-S., Yeow, K., & Dérijard, B. (2004) · Cellular Signalling · doi:10.1016/j.cellsig.2004.05.003 · Cited by 52 · see citing works
de la Peña, J. B., Lee, H. L., Yoon, S. Y., et al. (2013) · Journal of Natural Medicines · doi:10.1007/s11418-013-0754-3 · Cited by 49 · see citing works
Self-citationPotential Biological Activities of Magnoflorine: A Compound from Aristolochia debilis Sieb. et Zucc
Li, C., & Wang, M. (2014) · 한국자원식물학회지 · doi:10.7732/kjpr.2014.27.3.223 · Cited by 42 · see citing works
Park, H. J., Kim, Y. J., Leem, K., et al. (2005) · Phytotherapy Research · doi:10.1002/ptr.1539 · Cited by 27 · see citing works
The p38 MAPK family, a pushmi-pullyu of skeletal muscle differentiation
Lassar, A. B. (2009) · The Journal of Cell Biology · doi:10.1083/jcb.200911123 · Cited by 25 · see citing works
Tetrahydropalmatine Promotes Myoblast Differentiation through Activation of p38MAPK and MyoD
Lee, S.-J., Yoo, M., Go, G.-Y., Hwang, J., Lee, H.-G., Kim, Y.-K., Seo, D.-W., Baek, N.-I., Ryu, J.-H., Kang, J.-S., et al. (2014) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2014.10.115 · Cited by 25 · see citing works
Self-citationDehydrocorydaline promotes myogenic differentiation via p38 MAPK activation
Yoo, M., Lee, S., Kim, Y. K., Seo, D. W., Baek, N., Ryu, J., Kang, J., & Bae, G. (2016) · Molecular Medicine Reports · doi:10.3892/mmr.2016.5653 · Cited by 25 · see citing works
Self-citationHesperedin promotes MyoD‐induced myogenic differentiation in vitro and in vivo
Jeong, H., Lee, J. Y., Jang, E. J., et al. (2011) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2011.01243.x · Cited by 19 · see citing works
Bakuchiol augments MyoD activation leading to enhanced myoblast differentiation
Lee, S.-J., Yoo, M., Go, G.-Y., Kim, D. H., Choi, H., Leem, Y.-E., Kim, Y. K., Seo, D. W., Ryu, J., Kang, J., et al. (2016) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2016.02.008 · Cited by 17 · see citing works
Self-citationGong, Z., Chen, Y., Zhang, R., et al. (2014) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2014/845048 · Cited by 15 · see citing works
Sakumoto, H., Yokota, Y., Ishibashi, G., et al. (2015) · Journal of Natural Medicines · doi:10.1007/s11418-015-0907-7 · Cited by 10 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy
Wang, Y., Liu, Q., Quan, H., Kang, S.-G., Huang, K., & Tong, T. (2021) · Nutrients · doi:10.3390/nu13061914 · Cited by 61 · see citing works
Yadav, A., Singh, A., Phogat, J., Dahuja, A., & Dabur, R. (2021) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2020.113510 · Cited by 49 · see citing works
- Authors
- Lee, H., Tuong, L. T., Jeong, J. H., Lee, S.-J., Bae, G.-U., & Ryu, J.-H.
- Affiliations
- Sookmyung Women's University (KR)
- Corresponding author
- Ryu, Jae-Ha
- Venue
- Bioorganic & Medicinal Chemistry Letters
- Publisher
- Elsevier BV
- Published
- 2017-03
- Volume
- 27
- Issue
- 6
- Pages
- 1401-1404
- ISSN
- 0960-894X (ISSN-L)
- OpenAlex
- W2584782740
- Cited by
- 19
- References
- 29
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 5 · 2019: 1 · 2018: 2
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioorganic & Medicinal Chemistry Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation of Korea (2011-0030074, 2015R1D1A4A01019006)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Pharmacology › Berberine and alkaloids research
- Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
- Keywords
- Coptis japonica, isoquinoline alkaloids, magnoflorine, myogenesis, muscle atrophy, sarcopenia, cachexia, p38 MAPK, Akt signaling, MyoD, myosin heavy chain, C2C12 myoblasts, myotubes
- MeSH terms
- Alkaloids, Animals, Cell Differentiation, Cell Line, Myoblasts, Coptis, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review