Cited by 19Muscle Physiology and DisordersBerberine and alkaloids researchTraditional Chinese Medicine Analysis

Isoquinoline Alkaloids from Coptis Japonica Stimulate the Myoblast Differentiation Via P38 MAP-Kinase and Akt Signaling Pathway

Lee, Heyjin, Tuong, Le Thi, Jeong, Ji Hye, Lee, Sang-Jin, Bae, Gyu-Un, Ryu, Jae-Ha·2017·Bioorganic & Medicinal Chemistry Letters

Abstract

To overcome the muscle atrophy, such as cachexia and sarcopenia, we tried to find myogenic agents from medicinal plants. From myogenic extract of Coptis japonica, we purified six isoquinoline alkaloids and evaluated their effects on transactivation of myoD and MHC expression in C2C12 cells during differentiation process. Among obtained compounds, magnoflorine most efficiently enhanced the myoblast differentiation by activating the p38 MAP kinase and Akt pathway, and also increased the number of multinucleated and cylinder-shaped myotubes. These results propose that magnoflorine from Coptis japonica might be a promising lead compound for the development of anti-muscle atrophy drug.

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Crossref · 10.1016/j.bmcl.2017.02.003

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  • Isoquinoline alkaloids from Coptis japonica stimulate myoblast differentiation by promoting MyoD transcriptional activity.

    “For example, isoquinoline alkaloids from Coptis japonica stimulate myoblast differentiation by promoting MyoD transcriptional activity.”
  • Magnoflorine increases expression of MyoD and MHC and stimulates myoblast differentiation via the activation of p38 MAPK and Akt signaling in C2C12 myotubes.

    “Magnoflorine increases expression of MyoD and MHC and subsequently stimulates myoblast differentiation via the activation of p38 MAPK and Akt signaling in C2C12 myotubes [132].”

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Authors
Lee, H., Tuong, L. T., Jeong, J. H., Lee, S.-J., Bae, G.-U., & Ryu, J.-H.
Affiliations
Sookmyung Women's University (KR)
Corresponding author
Ryu, Jae-Ha
Venue
Bioorganic & Medicinal Chemistry Letters
Publisher
Elsevier BV
Published
2017-03
Volume
27
Issue
6
Pages
1401-1404
ISSN
0960-894X (ISSN-L)
OpenAlex
W2584782740
Cited by
19
References
29
Citations by year
2026: 2 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 2 · 2020: 5 · 2019: 1 · 2018: 2
FWCI
0.89
Type
journal article
Language
en
Open access
closed
Locations
  • Bioorganic & Medicinal Chemistry Letters (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Research Foundation of Korea (2011-0030074, 2015R1D1A4A01019006)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Pharmacology › Berberine and alkaloids research
  • Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
Keywords
Coptis japonica, isoquinoline alkaloids, magnoflorine, myogenesis, muscle atrophy, sarcopenia, cachexia, p38 MAPK, Akt signaling, MyoD, myosin heavy chain, C2C12 myoblasts, myotubes
MeSH terms
Alkaloids, Animals, Cell Differentiation, Cell Line, Myoblasts, Coptis, Mice

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