The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Deconvolution-free Subcellular Imaging with Axially Swept Light Sheet Microscopy
Open accessAbstract
The use of propagation invariant Bessel beams has enabled high-resolution subcellular light sheet fluorescence microscopy. However, the energy within the concentric side lobe structure of Bessel beams increases significantly with propagation length, generating unwanted out-of-focus fluorescence that enforces practical limits on the imaging field of view size. Here, we present a light sheet fluorescence microscope that achieves 390 nm isotropic resolution and high optical sectioning strength (i.e., out-of-focus blur is strongly suppressed) over large field of views, without the need for structured illumination or deconvolution-based postprocessing. We demonstrate simultaneous dual-color, high-contrast, and high-dynamic-range time-lapse imaging of migrating cells in complex three-dimensional microenvironments, three-dimensional tracking of clathrin-coated pits, and long-term imaging spanning >10 h and encompassing >2600 time points.
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Robust single-particle tracking in live-cell time-lapse sequences
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Self-citationA tense situation: forcing tumour progression
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Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
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Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy
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Whole-brain functional imaging at cellular resolution using light-sheet microscopy
Ahrens, M. B., Orger, M. B., Robson, D. N., Li, J. M., & Keller, P. J. (2013) · Nature Methods · doi:10.1038/nmeth.2434 · Cited by 1,392
Plasticity of cell migration: a multiscale tuning model
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A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum
Shaner, N. C., Lambert, G. G., Chammas, A., Ni, Y., Cranfill, P. J., Baird, M. A., Sell, B. R., Allen, J. R., Day, R. N., Israelsson, M., et al. (2013) · Nature Methods · doi:10.1038/nmeth.2413 · Cited by 1,289
Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination
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Improving the photostability of bright monomeric orange and red fluorescent proteins
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Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0
Voleti, V., Patel, K. B., Li, W., Perez Campos, C., Bharadwaj, S., Yu, H., Ford, C., Casper, M. J., Yan, R. W., Liang, W., et al. (2019) · Nature Methods · doi:10.1038/s41592-019-0579-4 · Cited by 320
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Metadata
- Authors
- Dean, K. M., Roudot, P., Welf, E. S., Danuser, G., & Fiolka, R.
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 2015-06
- Volume
- 108
- Issue
- 12
- Pages
- 2807-2815
- ISSN
- 0006-3495 (ISSN-L)
- OpenAlex
- W1487216741
- Cited by
- 253
- References
- 33
- FWCI
- 5.71
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Dean, K. M., Roudot, P., Welf, E. S., Danuser, G., & Fiolka, R. (2015). Deconvolution-free Subcellular Imaging with Axially Swept Light Sheet Microscopy. Biophysical Journal. https://doi.org/10.1016/j.bpj.2015.05.013
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