Characterizing Cellular Biophysical Responses to Stress by Relating Density, Deformability, and Size
Open accessAbstract
Cellular physical properties are important indicators of specific cell states. Although changes in individual biophysical parameters, such as cell size, density, and deformability, during cellular processes have been investigated in great detail, relatively little is known about how they are related. Here, we use a suspended microchannel resonator (SMR) to measure single-cell density, volume, and passage time through a narrow constriction of populations of cells subjected to a variety of environmental stresses. Osmotic stress significantly affects density and volume, as previously shown. In contrast to density and volume, the effect of an osmotic challenge on passage time is relatively small. Deformability, as determined by comparing passage times for cells with similar volume, exhibits a strong dependence on osmolarity, indicating that passage time alone does not always provide a meaningful proxy for deformability. Finally, we find that protein synthesis inhibition, cell-cycle arrest, protein kinase inhibition, and cytoskeletal disruption result in unexpected relationships among deformability, density, and volume. Taken together, our results suggest that by measuring multiple biophysical parameters, one can detect unique characteristics that more specifically reflect cellular behaviors.
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The SMR design and operation were described in earlier work.
“The design and operation of the SMR have been previously described13–15.”
From Drug sensitivity of single cancer cells is predicted by changes in mass accumulation rate (2016) · cites this paper as [14]
The SMR passes single cells in suspension and produces a frequency shift proportional to cell buoyant mass.
“In short, single cells in suspension are passed through the SMR resulting in a frequency shift that is proportional to cell buoyant mass.”
From Drug sensitivity of single cancer cells is predicted by changes in mass accumulation rate (2016) · cites this paper as [14]
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Enhancing cell characterization via hydrodynamic compression in suspended microchannel resonators
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The impacts of shape in lateral migration of cancer cells in a microchannelPreprint
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- Authors
- Byun, S., Hecht, V. C., & Manalis, S. R.
- Affiliations
- Koch Institute for Integrative Cancer Research At MIT (US)
- Massachusetts Institute of Technology (US)
- Corresponding author
- Manalis, Scott R.
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 2015-10
- Volume
- 109
- Issue
- 8
- Pages
- 1565-1573
- ISSN
- 0006-3495 (ISSN-L)
- PMC
- PMC4624161
- OpenAlex
- W1794742234
- Cited by
- 34
- References
- 67
- Citations by year
- 2025: 4 · 2024: 3 · 2023: 3 · 2022: 7 · 2021: 4 · 2020: 4 · 2019: 3 · 2018: 1 · 2017: 2 · 2016: 3
- FWCI
- 1.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biophysical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc-nd)
- Funders
- U.S. Public Health Service (26697290-47281-A)
- National Cancer Institute (U54CA143874, U54CA151459)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Keywords
- cell deformability, cell density, cell volume, osmotic stress, suspended microchannel resonators, passage time, cell size, protein synthesis inhibition, cell cycle arrest, protein kinase inhibition, cytoskeleton disruption, cellular stress response
- MeSH terms
- Animals, Cell Line, Liver, Osmotic Pressure, Stress, Physiological, Microfluidics, Microfluidic Analytical Techniques, Cell Size, Mice
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