Dissecting temporal and spatial control of cytokinesis with a myosin II Inhibitor
Abstract
Completion of cell division during cytokinesis requires temporally and spatially regulated communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell membrane. We identified a specific inhibitor of nonmuscle myosin II, blebbistatin, that inhibited contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Using blebbistatin and other drugs, we showed that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis. Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.
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Confirmation of F-Actin-myosin interaction during Tau-induced PM blebbing was obtained when cells were incubated with Blebbistatin, a specific inhibitor of Myosin-II activity, and neither F-Actin remodeling nor PM blebs formation were observed.
“Confirmation of F-Actin-myosin interaction during Tau-induced PM blebbing was obtained when cells were incubated with Blebbistatin, a specific inhibitor of Myosin-II activity [49], and neither F-Actin remodeling nor PM blebs formation were observed (Fig. 4C).”
From Expression of Tau Produces Aberrant Plasma Membrane Blebbing in Glial Cells Through RhoA-ROCK-Dependent F-Actin Remodeling (2016) · cites this paper as [49]
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- Authors
- Straight, A. F., Cheung, A., Limouze, J., Chen, I., Westwood, N. J., Sellers, J. R., & Mitchison, T. J.
- Affiliations
- Harvard University (US)
- National Institutes of Health (US)
- Massachusetts General Hospital (US)
- School of Chemistry, University of St. Andrews, St. Andrews, Fife, KY16 9AJ, UK.
- Corresponding author
- Straight, Aaron F.
- Venue
- Science
- Published
- 2003-03-14
- Volume
- 299
- Issue
- 5613
- Pages
- 1743-1747
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1980056833
- Cited by
- 1,412
- References
- 0
- Citations by year
- 2026: 31 · 2025: 43 · 2024: 48 · 2023: 49 · 2022: 39 · 2021: 40 · 2020: 62 · 2019: 38 · 2018: 63 · 2017: 61 · 2016: 69 · 2015: 63 · 2014: 74 · 2013: 62 · 2012: 74
- FWCI
- 24.12
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM 62566, GM 23928, P01GM062566, R01GM023928)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- cytokinesis, non-muscle myosin II, blebbistatin, cleavage furrow, contractile ring, ubiquitin-mediated proteolysis, cell cycle exit, actin cytoskeleton, cell division
- MeSH terms
- Anaphase, Cell Division, Cell Movement, Contractile Proteins, DNA Replication, Enzyme Inhibitors, HeLa Cells, Heterocyclic Compounds, 4 or More Rings, Humans, Leupeptins, Microtubules, Mitosis, Nocodazole, Kinesins, Protein Serine-Threonine Kinases, Cyclin-Dependent Kinases, Staurosporine, Myosin Type II, Ubiquitin, Aurora Kinases
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