Expression of sphingomyelin synthase 1 gene in rat brain focal ischemia
Abstract
Metabolites of the sphingomyelin cycle are reported to play an important role in neuronal death after ischemia. To elucidate the involvement of the key enzyme of this cycle, sphingomyelin synthase (SMS), in the mechanism underlying cerebral ischemia, we, for the first time, investigated changes in the mRNA expression of the SMS1 gene in rats after focal cerebral ischemia. According to our histological analysis, the damaged area is localized only in the ipsilateral cortex. In the ischemic cortex, the level of SMS1 transcripts was decreased at 3 and 24 h after occlusion, and at 72 h it had returned to the control level. A reduced level of SMS1 mRNA expression in the subcortex of rats with occlusion and sham-operated animals also was appeared during the first 24 h after surgery. This could be attributed to the effect of surgical stress. Seventy-two hours after occlusion, SMS1 mRNA expression in subcortex of ischemic rats was still at a decreased level; this may be considered to be a somewhat distant extended effect. Our results show the early response of the SMS1 gene that can be induced by both ischemia and stress. The results also suggest that inhibition of SMS1 mRNA expression may contribute to ceramide accumulation in a damaged cortex.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The damaged area was localized in the ipsilateral frontoparietal cortex of the ischemic animals.
“A histological analysis showed the damaged area was localized in the ipsilateral frontoparietal cortex of the ischemic animals (Fig. 1; Dmitrieva et al. 2008a).”
From Semax and Pro-Gly-Pro activate the transcription of neurotrophins and their receptor genes after cerebral ischemia (2010) · cites this paper as Dmitrieva et al. 2008a
Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,240 · see citing works
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczyński, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 47,092 · see citing works
The rat brain in stereotaxic coordinates
Palkovits, M. (1983) · Neuropeptides · doi:10.1016/0143-4179(83)90049-5 · Cited by 28,959 · see citing works
The Rat Brain in Stereotaxic Coordinates
Paxinos, G., & Watson, C. (1983) · Elsevier eBooks · doi:10.1016/c2009-0-63235-9 · Cited by 28,585 · see citing works
Ischemic Cell Death in Brain Neurons
Lipton, P. (1999) · Physiological Reviews · doi:10.1152/physrev.1999.79.4.1431 · Cited by 3,043 · see citing works
Rodent models of focal stroke: Size, mechanism, and purpose
Carmichael, S. T. (2005) · NeuroRx · doi:10.1602/neurorx.2.3.396 · Cited by 710 · see citing works
Identification of a family of animal sphingomyelin synthases
Huitema, K., van den Dikkenberg, J. B., Brouwers, J. F. H. M., & Holthuis, J. C. M. (2003) · The EMBO Journal · doi:10.1038/sj.emboj.7600034 · Cited by 613 · see citing works
Enzymes of Sphingolipid Metabolism: From Modular to Integrative Signaling
Hannun, Y. A., Luberto, C., & Argraves, K. M. (2001) · Biochemistry · doi:10.1021/bi002836k · Cited by 502 · see citing works
Progression from ischemic injury to infarct following middle cerebral artery occlusion in the rat.
García, J. H., Yoshida, Y., Chen, H., Li, Y., Zhang, Z. G., Lian, J., Chen, S., & Chopp, M. (1993) · PubMed Central · Cited by 494 · see citing works
Ceramide regulates cellular homeostasis via diverse stress signaling pathways
Ruvolo, P. (2001) · Leukemia · doi:10.1038/sj.leu.2402197 · Cited by 203 · see citing works
Cellular and enzymic synthesis of sphingomyelin
Voelker, D. R., & Kennedy, E. P. (1982) · Biochemistry · doi:10.1021/bi00540a027 · Cited by 192 · see citing works
Genomics of the Periinfarction Cortex after Focal Cerebral Ischemia
Lu, A., Tang, Y., Ran, R., Clark, J. F., Aronow, B. J., & Sharp, F. R. (2003) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/01.wcb.0000062340.80057.06 · Cited by 176 · see citing works
Bioactive sphingolipids in the modulation of the inflammatory response
El‐Alwani, M., Wu, B. X., Obeid, L. M., & Hannun, Y. A. (2006) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2006.04.004 · Cited by 165 · see citing works
Nakane, M., Kubota, M., Nakagomi, T., Tamura, A., Hisaki, H., Shimasaki, H., & Ueta, N. (2000) · Neuroscience Letters · doi:10.1016/s0304-3940(00)01655-4 · Cited by 76 · see citing works
Upregulation of ceramide and its regulating mechanism in a rat model of chronic cerebral ischemia
Ohtani, R., Tomimoto, H., Kondo, T., Wakita, H., Akiguchi, I., Shibasaki, H., & Okazaki, T. (2004) · Brain Research · doi:10.1016/j.brainres.2004.07.024 · Cited by 65 · see citing works
Sphingomyelin changes in rat cerebral cortex during focal ischemia
Kubota, M., Narita, K., Nakagomi, T., Tamura, A., Shimasaki, H., Ueta, N., & Yoshida, S. (1996) · Neurological Research · doi:10.1080/01616412.1996.11740432 · Cited by 58 · see citing works
Identification of mouse sphingomyelin synthase 1 as a suppressor of Bax-mediated cell death in yeast
Yang, Z., Khoury, C. M., Jean-Baptiste, G., & Greenwood, M. T. (2006) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2006.00052.x · Cited by 55 · see citing works
Marggraf, W.-D., & Kanfer, J. N. (1984) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(84)90248-0 · Cited by 51 · see citing works
Inhibition of sphingomyelinase activity helps to prevent neuron death caused by ischemic stress
SOEDA, S. (2004) · Neurochemistry International · doi:10.1016/j.neuint.2004.04.001 · Cited by 48 · see citing works
Sidedness of ceramide-phosphoethanolamine synthesis on rat liver and brain microsomal membranes.
Malgat, M., Maurice, A., & Baraud, J. (1987) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38715-0 · Cited by 21 · see citing works
Human gene MOB: structure specification and aspects of transcriptional activity
Vladychenskaya, I. P., Dergunova, L. V., Dmitrieva, V. G., & Limborska, S. A. (2004) · Gene · doi:10.1016/j.gene.2004.06.003 · Cited by 18 · see citing works
Self-citationRegulation of the sphingolipid signaling pathways in the growing and hypoxic rat heart
El‐Alwani, M., Usta, J. A., Nemer, G. M., El-Sabban, M. E., Nasser, M., Bitar, H., Souki, R., Dbaibo, G. S., & Bitar, F. F. (2005) · Prostaglandins & Other Lipid Mediators · doi:10.1016/j.prostaglandins.2005.09.002 · Cited by 14 · see citing works
Vladychenskaya, I. P., Dergunova, L. V., & Sa, L. (2002) · Biomolecular Engineering · doi:10.1016/s1389-0344(01)00110-1 · Cited by 12 · see citing works
Self-citationDergunova, L. V., Ip, V., Lg, P., Nm, R., Gp, L., & Sa, L. (1998) · PubMed · Cited by 7 · see citing works
Self-citationDergunova, L. V., Nm, R., Vladychenskaya, I. P., & Limborska, S. A. (2003) · Molecular Biology · doi:10.1023/a:1023353907095 · Cited by 6 · see citing works
Self-citationDergunova, L. V., Raevskaya, N. M., Vladychenskaya, I. P., & Limborska, S. A. (2003) · Biomolecular Engineering · doi:10.1016/s1389-0344(03)00003-0 · Cited by 5 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Regulation of sphingomyelin metabolism
Bienias, K., Fiedorowicz, A., Sadowska, A. J., Prokopiuk, S., & Car, H. (2016) · Pharmacological Reports · doi:10.1016/j.pharep.2015.12.008 · Cited by 193 · see citing works
Genomic approach to selective vulnerability of the hippocampus in brain ischemia–hypoxia
Schmidt‐Kastner, R. (2015) · Neuroscience · doi:10.1016/j.neuroscience.2015.08.034 · Cited by 138 · see citing works
Ceramide Metabolism and Parkinson’s Disease—Therapeutic Targets
Custodia, A., Aramburu-Núñez, M., Correa‐Paz, C., Posado-Fernández, A., Gómez-Larrauri, A., Castillo, J., Gómez‐Muñoz, A., Sobrino, T., & Ouro, A. (2021) · Biomolecules · doi:10.3390/biom11070945 · Cited by 63 · see citing works
Dmitrieva, V. G., Povarova, O. V., Skvortsova, V. I., Limborska, S. A., Myasoedov, N. F., & Dergunova, L. V. (2010) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-009-9432-0 · Cited by 54 · see citing works
Self-citationMedvedeva, E. V., Dmitrieva, V. G., Povarova, O. V., Limborska, S. A., Skvortsova, V. I., Myasoedov, N. F., & Dergunova, L. V. (2014) · BMC Genomics · doi:10.1186/1471-2164-15-228 · Cited by 33 · see citing works
Self-citationChanges in the metabolism of sphingolipids after subarachnoid hemorrhage
Testai, F. D., Xu, H., Kilkus, J. P., Suryadevara, V., Gorshkova, I. A., Berdyshev, E. V., Pelligrino, D. A., & Dawson, G. (2015) · Journal of Neuroscience Research · doi:10.1002/jnr.23542 · Cited by 30 · see citing works
Changes in the Cerebrospinal Fluid Ceramide Profile After Subarachnoid Hemorrhage
Testai, F. D., Hillmann, M. L., Amin‐Hanjani, S., Gorshkova, I. A., Berdyshev, E. V., Gorelick, P. B., & Dawson, G. (2012) · Stroke · doi:10.1161/strokeaha.112.650390 · Cited by 30 · see citing works
Sphingomyelin Synthases in Neuropsychiatric Health and Disease
Mühle, C., Canalejas, R. D. B., & Kornhuber, J. (2019) · Neurosignals · doi:10.33594/000000200 · Cited by 29 · see citing works
Involvement of Ceramide Metabolism in Cerebral Ischemia
Ouro, A., Correa‐Paz, C., Maqueda, E., Custodia, A., Aramburu-Núñez, M., Romaus‐Sanjurjo, D., Posado-Fernández, A., Candamo-Lourido, M., Alonso-Alonso, M. L., Hervella, P., et al. (2022) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2022.864618 · Cited by 26 · see citing works
Medvedeva, E. V., Dmitrieva, V. G., Limborska, S. A., Myasoedov, N. F., & Dergunova, L. V. (2017) · Molecular Genetics and Genomics · doi:10.1007/s00438-017-1297-1 · Cited by 23 · see citing works
Self-citationDmitrieva, V. G., Stavchansky, V. V., Поварова, О. В., Skvortsova, V. I., Limborska, S. A., & Dergunova, L. V. (2016) · Molecular Biology · doi:10.1134/s0026893316030067 · Cited by 20 · see citing works
Self-citationSphingolipids in spinal cord injury.
Jones, Z. B., & Ren, Y. (2016) · PubMed Central · Cited by 19 · see citing works
Medvedeva, E. V., Dmitrieva, V. G., Povarova, O. V., Limborska, S. A., Skvortsova, V. I., Myasoedov, N. F., & Dergunova, L. V. (2013) · Journal of Molecular Neuroscience · doi:10.1007/s12031-012-9853-y · Cited by 18 · see citing works
Self-citationDmitrieva, V. G., Dergunova, L. V., Поварова, О. В., Skvortsova, V. I., Limborskaya, S. A., & Myasoedov, N. F. (2008) · Doklady Biochemistry and Biophysics · doi:10.1134/s1607672908050037 · Cited by 16 · see citing works
Self-citationPharmacotranscriptomics of peptide drugs with neuroprotective properties
Dergunova, L. V., Filippenkov, I. B., Limborska, S. A., & Myasoedov, N. F. (2020) · Medicinal Research Reviews · doi:10.1002/med.21704 · Cited by 15 · see citing works
Self-citationTripeptide Pro-Gly-Pro affects rat-brain transcriptome during focal ischemia
Medvedeva, E. V., Dmitrieva, V. G., Поварова, О. В., Limborska, S. A., Skvortsova, V. I., Myasoedov, N. F., & Dergunova, L. V. (2014) · Molecular Biology · doi:10.1134/s0026893314020125 · Cited by 8 · see citing works
Self-citationSemax-Induced Changes in Growth Factor mRNA Levels in the Rat Brain on the Third Day After Ischemia
Medvedeva, E. V., Dmitrieva, V. G., Stavchansky, V. V., Поварова, О. В., Limborska, S. A., Myasoedov, N. F., & Dergunova, L. V. (2015) · International Journal of Peptide Research and Therapeutics · doi:10.1007/s10989-015-9498-0 · Cited by 1 · see citing works
Self-citationStavchansky, V. V., Kurichenkova, E. O., Dmitrieva, V. G., Myasoedov, N. F., Limborska, S. A., & Dergunova, L. V. (2016) · Molecular Genetics Microbiology and Virology · doi:10.3103/s0891416816040078 · Cited by 1 · see citing works
Self-citationStavchansky, V. V., Kurichenkova, E. O., Dmitrieva, V. G., Мясоедов, Н. Ф., Limborska, S. A., & Dergunova, L. V. (2016) · Molecular Genetics Microbiology and Virology (Russian) · doi:10.18821/0208-0613-2016-34-4-137-142 · Cited by 1 · see citing works
Self-citation
- Authors
- Dmitrieva, V. G., Torshina, E. V., Yuzhakov, V. V., Povarova, O. V., Skvortsova, V. I., Limborska, S. A., & Dergunova, L. V.
- Affiliations
- Russian Academy of Sciences (RU)
- Medical Radiological Research Center (RU)
- Pirogov Russian National Research Medical University (RU)
- Corresponding author
- Dmitrieva, Veronika G.
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 2008-01
- Volume
- 1188
- Pages
- 222-227
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2094464942
- Cited by
- 19
- References
- 26
- Citations by year
- 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 1 · 2016: 5 · 2015: 3 · 2014: 2 · 2012: 2
- FWCI
- 0.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Russian Foundation for Basic Research
- Russian Academy of Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Health Sciences › Medicine › Physiology › Lysosomal Storage Disorders Research
- Keywords
- sphingomyelin synthase 1, focal cerebral ischemia, gene expression, mRNA expression, neuronal cell death, sphingomyelin cycle, ceramide accumulation, surgical stress, ischemic cortex, rats
- MeSH terms
- Animals, Ceramides, Cerebral Cortex, Cerebral Infarction, Brain Ischemia, Male, RNA, Messenger, Sphingomyelins, Stress, Physiological, Down-Regulation, Gene Expression Regulation, Enzymologic, Rats, Wistar, Transferases (Other Substituted Phosphate Groups), Oxidative Stress, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review