Cited by 19Sphingolipid Metabolism and SignalingNeurological Disorders and TreatmentsLysosomal Storage Disorders Research

Expression of sphingomyelin synthase 1 gene in rat brain focal ischemia

Dmitrieva, Veronika G., Torshina, Elena V., Yuzhakov, Vadim V., Povarova, Oksana V., Skvortsova, Veronika I., Limborska, Svetlana A., Dergunova, Lyudmila V.·2008·Brain Research

Abstract

Metabolites of the sphingomyelin cycle are reported to play an important role in neuronal death after ischemia. To elucidate the involvement of the key enzyme of this cycle, sphingomyelin synthase (SMS), in the mechanism underlying cerebral ischemia, we, for the first time, investigated changes in the mRNA expression of the SMS1 gene in rats after focal cerebral ischemia. According to our histological analysis, the damaged area is localized only in the ipsilateral cortex. In the ischemic cortex, the level of SMS1 transcripts was decreased at 3 and 24 h after occlusion, and at 72 h it had returned to the control level. A reduced level of SMS1 mRNA expression in the subcortex of rats with occlusion and sham-operated animals also was appeared during the first 24 h after surgery. This could be attributed to the effect of surgical stress. Seventy-two hours after occlusion, SMS1 mRNA expression in subcortex of ischemic rats was still at a decreased level; this may be considered to be a somewhat distant extended effect. Our results show the early response of the SMS1 gene that can be induced by both ischemia and stress. The results also suggest that inhibition of SMS1 mRNA expression may contribute to ceramide accumulation in a damaged cortex.

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Crossref · 10.1016/j.brainres.2007.10.056

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Authors
Dmitrieva, V. G., Torshina, E. V., Yuzhakov, V. V., Povarova, O. V., Skvortsova, V. I., Limborska, S. A., & Dergunova, L. V.
Affiliations
  • Russian Academy of Sciences (RU)
  • Medical Radiological Research Center (RU)
  • Pirogov Russian National Research Medical University (RU)
Corresponding author
Dmitrieva, Veronika G.
Venue
Brain Research
Publisher
Elsevier BV
Published
2008-01
Volume
1188
Pages
222-227
ISSN
0006-8993 (ISSN-L)
OpenAlex
W2094464942
Cited by
19
References
26
Citations by year
2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2017: 1 · 2016: 5 · 2015: 3 · 2014: 2 · 2012: 2
FWCI
0.26
Type
journal article
Language
en
Open access
closed
Locations
  • Brain Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Russian Foundation for Basic Research
  • Russian Academy of Sciences
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
  • Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
  • Health Sciences › Medicine › Physiology › Lysosomal Storage Disorders Research
Keywords
sphingomyelin synthase 1, focal cerebral ischemia, gene expression, mRNA expression, neuronal cell death, sphingomyelin cycle, ceramide accumulation, surgical stress, ischemic cortex, rats
MeSH terms
Animals, Ceramides, Cerebral Cortex, Cerebral Infarction, Brain Ischemia, Male, RNA, Messenger, Sphingomyelins, Stress, Physiological, Down-Regulation, Gene Expression Regulation, Enzymologic, Rats, Wistar, Transferases (Other Substituted Phosphate Groups), Oxidative Stress, Rats

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