Nociceptive Spinal Cord Neurons of Laminae I-III Exhibit Oxidative Stress Damage during Diabetic Neuropathy Which Is Prevented by Early Antioxidant Treatment with Epigallocatechin-Gallate (EGCG)
Abstract
Spinal cord neurons located in laminae I-III respond to nociceptive stimuli and participate in the transmission of painful information to the brain. In the present study we evaluated if nociceptive laminae I-III neurons are affected by oxidative stress damage in a model of diabetic neuropathic pain (DNP), the streptozotocin-induced diabetic rat (STZ rat). Additionally, we evaluated the effects of a preventive antioxidant treatment with epigallocatechin-gallate (EGCG) in nociceptive neuronal activation and behavioural signs of DNP. Three days after diabetes induction, a treatment protocol of STZ rats with an aqueous solution of EGCG in the drinking water was initiated. Ten weeks after the onset of treatment, the spinal cords were immunoreacted against validated markers of oxidative stress damage (8-hydroxy-2'-deoxyguanosine; 8-OHdG) and of nociceptive neuronal activation (Fos). Mechanical hypersensitivity was assessed before and after EGCG treatment. Untreated STZ rats presented increased levels of 8-OHdG immunoreaction, higher numbers of Fos-immunoreacted neurons and high levels of co-localization of 8-OHdG and Fos in laminae I-III. Treatment with EGCG normalized the increase of the above mentioned parameters and ameliorated mechanical hypersensitivity. The present study shows that nociceptive neurons in spinal cord laminae I-III exhibit oxidative stress damage during diabetic neuropathy, which probably affects ascending pain transmission during DNP. The neurobiological mechanisms and translational perspectives of the beneficial effects of a preventive and sustained EGCG treatment in DNP need to be evaluated in the future.
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EGCG affects diabetes-related comorbidities such as diabetic neuropathy.
“to affect diabetes-related comorbidities such as diabetic neuropathy (Raposo et al., 2015)”
From Exploring the Therapeutic Potential of Phytochemicals in Alzheimer’s Disease: Focus on Polyphenols and Monoterpenes (2022) · cites this paper as Raposo et al., 2015
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- Authors
- Raposo, D., Morgado, C., Pereira-Terra, P., & Tavares, I.
- Affiliations
- Universidade do Porto (PT)
- Corresponding author
- Tavares, I.
- Venue
- Brain Research Bulletin
- Publisher
- Elsevier BV
- Published
- 2015-01
- Volume
- 110
- Pages
- 68-75
- ISSN
- 0361-9230 (ISSN-L)
- OpenAlex
- W1972699960
- Cited by
- 61
- References
- 66
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 9 · 2023: 4 · 2022: 8 · 2021: 4 · 2020: 6 · 2019: 3 · 2018: 5 · 2017: 4 · 2016: 5 · 2015: 4
- FWCI
- 2.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research Bulletin (publishedVersion)
- PubMed (publishedVersion)
- Funders
- EU Project REDDSTAR (FP7-Health-2012.2.4.3-1) (IJUP/UNICER 2010)
- Topics
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Anesthesiology and Pain Medicine › Pain Management and Treatment
- Keywords
- diabetic neuropathic pain, oxidative stress, nociceptors, epigallocatechin gallate, antioxidant treatment, 8-hydroxy-2'-deoxyguanosine, Fos-like immunoreactivity, mechanical hypersensitivity, streptozotocin-induced diabetes
- MeSH terms
- 8-Hydroxy-2'-Deoxyguanosine, Animals, Antioxidants, Catechin, Deoxyguanosine, Diabetes Mellitus, Experimental, Diabetic Neuropathies, Hyperalgesia, Immunohistochemistry, Male, Neuralgia, Nociceptors, Spinal Cord, Touch, Proto-Oncogene Proteins c-fos, Rats, Wistar, Oxidative Stress, Neuroprotective Agents
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