Cited by 61Pain Mechanisms and TreatmentsNeuroscience and Neuropharmacology ResearchPain Management and Treatment

Nociceptive Spinal Cord Neurons of Laminae I-III Exhibit Oxidative Stress Damage during Diabetic Neuropathy Which Is Prevented by Early Antioxidant Treatment with Epigallocatechin-Gallate (EGCG)

Raposo, D., Morgado, C., Pereira-Terra, P., Tavares, I.·2015·Brain Research Bulletin

Abstract

Spinal cord neurons located in laminae I-III respond to nociceptive stimuli and participate in the transmission of painful information to the brain. In the present study we evaluated if nociceptive laminae I-III neurons are affected by oxidative stress damage in a model of diabetic neuropathic pain (DNP), the streptozotocin-induced diabetic rat (STZ rat). Additionally, we evaluated the effects of a preventive antioxidant treatment with epigallocatechin-gallate (EGCG) in nociceptive neuronal activation and behavioural signs of DNP. Three days after diabetes induction, a treatment protocol of STZ rats with an aqueous solution of EGCG in the drinking water was initiated. Ten weeks after the onset of treatment, the spinal cords were immunoreacted against validated markers of oxidative stress damage (8-hydroxy-2'-deoxyguanosine; 8-OHdG) and of nociceptive neuronal activation (Fos). Mechanical hypersensitivity was assessed before and after EGCG treatment. Untreated STZ rats presented increased levels of 8-OHdG immunoreaction, higher numbers of Fos-immunoreacted neurons and high levels of co-localization of 8-OHdG and Fos in laminae I-III. Treatment with EGCG normalized the increase of the above mentioned parameters and ameliorated mechanical hypersensitivity. The present study shows that nociceptive neurons in spinal cord laminae I-III exhibit oxidative stress damage during diabetic neuropathy, which probably affects ascending pain transmission during DNP. The neurobiological mechanisms and translational perspectives of the beneficial effects of a preventive and sustained EGCG treatment in DNP need to be evaluated in the future.

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Crossref · 10.1016/j.brainresbull.2014.12.004

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Authors
Raposo, D., Morgado, C., Pereira-Terra, P., & Tavares, I.
Affiliations
Universidade do Porto (PT)
Corresponding author
Tavares, I.
Venue
Brain Research Bulletin
Publisher
Elsevier BV
Published
2015-01
Volume
110
Pages
68-75
ISSN
0361-9230 (ISSN-L)
OpenAlex
W1972699960
Cited by
61
References
66
Citations by year
2026: 2 · 2025: 7 · 2024: 9 · 2023: 4 · 2022: 8 · 2021: 4 · 2020: 6 · 2019: 3 · 2018: 5 · 2017: 4 · 2016: 5 · 2015: 4
FWCI
2.88
Type
journal article
Language
en
Open access
closed
Locations
  • Brain Research Bulletin (publishedVersion)
  • PubMed (publishedVersion)
Funders
EU Project REDDSTAR (FP7-Health-2012.2.4.3-1) (IJUP/UNICER 2010)
Topics
  • Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Health Sciences › Medicine › Anesthesiology and Pain Medicine › Pain Management and Treatment
Keywords
diabetic neuropathic pain, oxidative stress, nociceptors, epigallocatechin gallate, antioxidant treatment, 8-hydroxy-2'-deoxyguanosine, Fos-like immunoreactivity, mechanical hypersensitivity, streptozotocin-induced diabetes
MeSH terms
8-Hydroxy-2'-Deoxyguanosine, Animals, Antioxidants, Catechin, Deoxyguanosine, Diabetes Mellitus, Experimental, Diabetic Neuropathies, Hyperalgesia, Immunohistochemistry, Male, Neuralgia, Nociceptors, Spinal Cord, Touch, Proto-Oncogene Proteins c-fos, Rats, Wistar, Oxidative Stress, Neuroprotective Agents

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