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Cited by 302

The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.

Reduced synchrony of Ca2+ release with loss of T-tubules—a comparison to Ca2+ release in human failing cardiomyocytes

Open access
Louch, W·2004·Cardiovascular Research
DOI 10.1016/j.cardiores.2003.12.031
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Abstract

OBJECTIVES: During cardiac excitation-contraction coupling, Ca2+ release from the sarcoplasmic reticulum (SR) occurs at the junctional complex with the T-tubules, containing the L-type Ca2+ channels. A partial loss of T-tubules has been described in myocytes from failing canine and human hearts. We examined how graded reduction of T-tubule density would affect the synchrony of Ca2+ release. METHODS: Adult pig ventricular myocytes were isolated and cultured for 24 and 72 h. T-tubules, visualized with di-8-ANEPPS, and [Ca2+]i transients (Fluo-3) were recorded during confocal line scan imaging. RESULTS: Cultured cardiomyocytes exhibited a progressive reduction in T-tubule density. [Ca2+]i transients showed small areas of delayed Ca2+ release which gradually increased in number and size with loss of T-tubules. Local [Ca2+]i transients in the delayed regions were reduced. Due to these changes, loss of T-tubules resulted in an overall slowing of the rise of [Ca2+] along the entire line scan and transient magnitude tended to be reduced, but there was no change in SR Ca2+ content. Human myocytes isolated from failing hearts had a T-tubule density comparable to that of freshly isolated pig myocytes. The size, but not the number, of delayed release areas tended to be larger. The overall rate of rise of [Ca2+]i was significantly faster than in pig myocytes with low T-tubule density. CONCLUSIONS: Loss of T-tubules reduces the synchrony of SR Ca2+ release. This could contribute to reduced efficiency of excitation-contraction coupling in heart failure, though dyssynchrony in human failing cells appears to be modest.

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Metadata

Authors
Louch, W.
Venue
Cardiovascular Research
Publisher
Oxford University Press (OUP)
Published
2004-04-01
Volume
62
Issue
1
Pages
63-73
ISSN
0008-6363 (ISSN-L)
DOI
10.1016/j.cardiores.2003.12.031
OpenAlex
W2003046582
Cited by
302
References
23
FWCI
8.47
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Reduced synchrony of Ca2+ release with loss of T-tubules—a comparison to Ca2+ release in human failing cardiomyocytes. https://app.cochat.ai/papers/10.1016/j.cardiores.2003.12.031
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Louch, W. (2004). Reduced synchrony of Ca2+ release with loss of T-tubules—a comparison to Ca2+ release in human failing cardiomyocytes. Cardiovascular Research. https://doi.org/10.1016/j.cardiores.2003.12.031

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