CoChat
Sign Up
Cited by 217Molecular Sensors and Ion DetectionAdvanced Fluorescence Microscopy TechniquesTrace Elements in Health

The DOI matches its Crossref record; the references it cites are not checked yet.

Imaging mobile zinc in biology

Open access
Tomat, Elisa, Lippard, Stephen J·2010·Current Opinion in Chemical Biology
DOI 10.1016/j.cbpa.2009.12.010PMC PMC2847655
Open this paper in CoChat — free account

Abstract

Trafficking and regulation of mobile zinc pools influence cellular functions and pathological conditions in multiple organs, including brain, pancreas, and prostate. The quest for a dynamic description of zinc distribution and mobilization in live cells fuels the development of increasingly sophisticated probes. Detection systems that respond to zinc binding with changes of their fluorescence emission properties have provided sensitive tools for mobile zinc imaging, and fluorescence microscopy experiments have afforded depictions of zinc distribution within live cells and tissues. Both small-molecule and protein-based fluorescent probes can address complex imaging challenges, such as analyte quantification, site-specific sensor localization, and real-time detection.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.cbpa.2009.12.010

Cites

0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

The publisher hasn’t deposited this paper’s reference list.

Research works printed but not linked: 52

  1. Maret, 2009, Coordination dynamics of zinc in proteins, Chem Rev, 109, 4682, 10.1021/cr800556u · DOI
  2. Eide, 2006, Zinc transporters and the cellular trafficking of zinc, Biochim Biophys Acta, 1763, 711, 10.1016/j.bbamcr.2006.03.005 · DOI · Paper page
  3. Franklin, 2009, The important role of the apoptotic effects of zinc in the development of cancers, J Cell Biochem, 106, 750, 10.1002/jcb.22049 · DOI
  4. Maret, 2009, Molecular aspects of human cellular zinc homeostasis: redox control of zinc potentials and zinc signals, Biometals, 22, 149, 10.1007/s10534-008-9186-z · DOI
  5. Frederickson, 2005, The neurobiology of zinc in health and disease, Nat Rev Neurosci, 6, 449, 10.1038/nrn1671 · DOI
  6. Taylor, 2005, Zinc, the pancreas, and diabetes: insights from rodent studies and future directions, Biometals, 18, 305, 10.1007/s10534-005-3686-x · DOI
  7. Costello, 2006, The clinical relevance of the metabolism of prostate cancer; zinc and tumor suppression: connecting the dots, Mol Cancer, 5, 17, 10.1186/1476-4598-5-17 · DOI
  8. Domaille, 2008, Synthetic fluorescent sensors for studying the cell biology of metals, Nat Chem Biol, 4, 168, 10.1038/nchembio.69 · DOI
  9. Que, 2008, Metals in neurobiology: probing their chemistry and biology with molecular imaging, Chem Rev, 108, 1517, 10.1021/cr078203u · DOI
  10. Carol, 2007, Ratiometric and near-infrared molecular probes for the detection and imaging of zinc ions, Chem Asian J, 2, 338, 10.1002/asia.200600370 · DOI
  11. Nolan, 2009, Small-molecule fluorescent sensors for investigating zinc metalloneurochemistry, Acc Chem Res, 42, 193, 10.1021/ar8001409 · DOI · Paper page
  12. McRae, 2009, In situ imaging of metals in cells and tissues, Chem Rev, 109, 4780, 10.1021/cr900223a · DOI
  13. Ketterman, 2008, Presynaptic evidence for zinc release at the mossy fiber synapse of rat hippocampus, J Neurosci Res, 86, 422, 10.1002/jnr.21488 · DOI
  14. Suh, 2009, Detection of zinc translocation into apical dendrite of CA1 pyramidal neuron after electrical stimulation, J Neurosci Methods, 177, 1, 10.1016/j.jneumeth.2008.09.016 · DOI
  15. Zhang, 2008, Ratiometric and water-soluble fluorescent zinc sensor of carboxamidoquinoline with an alkoxyethylamino chain as receptor, Org Lett, 10, 473, 10.1021/ol702869w · DOI
  16. Liu, 2007, Fluorescence sensing and binding behavior of aminobenzenesulfonamidoquinolino-beta-cyclodextrin to Zn(II), Org Lett, 9, 315, 10.1021/ol062816w · DOI
  17. Mikata, 2009, Bisquinoline-based fluorescent zinc sensors, Dalton Trans, 3800, 10.1039/b820763a · DOI
  18. Dhara, 2007, A two-dimensional coordination compound as a zinc ion selective luminescent probe for biological applications, Chem Asian J, 2, 1091, 10.1002/asia.200700152 · DOI
  19. Liu, 2009, A Zn(II) fluorescent sensor derived from 2-(pyridin-2-yl)benzoimidazole with ratiometric sensing potential, Org Lett, 11, 795, 10.1021/ol802537c · DOI
  20. Tamanini, 2009, A synthetically simple, click-generated cyclam-based zinc(II) sensor, Inorg Chem, 48, 319, 10.1021/ic8017634 · DOI
  21. Jiang, 2008, An NBD fluorophore-based sensitive and selective fluorescent probe for zinc ion, Chem Commun, 259, 10.1039/B712377A · DOI
  22. Qian, 2009, Visible light excitable Zn(II) fluorescent sensor derived from an intramolecular charge transfer fluorophore and its in vitro and in vivo application, J Am Chem Soc, 131, 1460, 10.1021/ja806489y · DOI
  23. Xu, 2009, An NBD-based colorimetric and fluorescent chemosensor for Zn(II) and its use for detection of intracellular zinc ions, Tetrahedron, 65, 2307, 10.1016/j.tet.2009.01.035 · DOI
  24. Wong, 2009, Subtle modification of 2,2-dipicolylamine lowers the affinity and improves the turn-on of Zn(II)-selective fluorescent sensors, Inorg Chem, 48, 7009, 10.1021/ic900990w · DOI
  25. Zhang, 2008, New strategy for quantifying biological zinc by a modified Zinpyr fluorescence sensor, J Am Chem Soc, 130, 15788, 10.1021/ja807156b · DOI
  26. Komatsu, 2007, Development of an iminocoumarin-based zinc sensor suitable for ratiometric fluorescence imaging of neuronal zinc, J Am Chem Soc, 129, 13447, 10.1021/ja072432g · DOI
  27. Mizukami, 2009, Design and synthesis of coumarin-based Zn(II) probes for ratiometric fluorescence imaging, Inorg Chem, 48, 7630, 10.1021/ic900247r · DOI
  28. Xue, 2009, A ratiometric fluorescent sensor with a large Stokes shift for imaging zinc ions in living cells, Chem Commun, 1061, 10.1039/B814501F · DOI
  29. Roussakis, 2008, ICPBCZin: a red emitting ratiometric fluorescent indicator with nanomolar affinity for Zn(II) ions, Cell Calcium, 44, 270, 10.1016/j.ceca.2007.12.008 · DOI
  30. Dong, 2009, Quinoline group modified carbon nanotubes for the detection of zinc ions, Nanoscale Res Lett, 4, 335, 10.1007/s11671-008-9248-8 · DOI
  31. Sarkar, 2009, Selective zinc(II)-ion fluorescence sensing by a functionalized mesoporous material covalently grafted with a fluorescent chromophore and consequent biological applications, Adv Funct Mater, 19, 223, 10.1002/adfm.200800888 · DOI
  32. Qiao, 2006, Zinc binding to a regulatory zinc-sensing domain monitored in vivo by using FRET, Proc Natl Acad Sci U S A, 103, 8674, 10.1073/pnas.0600928103 · DOI
  33. van Dongen, 2006, Ratiometric fluorescent sensor proteins with subnanomolar affinity for Zn(II) based on copper chaperone domains, J Am Chem Soc, 128, 10754, 10.1021/ja0610030 · DOI
  34. Dittmer, 2009, Genetically encoded sensors to elucidate spatial distribution of cellular zinc, J Biol Chem, 284, 16289, 10.1074/jbc.M900501200 · DOI
  35. Vinkenborg, 2009, Genetically encoded FRET sensors to monitor intracellular Zn(II) homeostasis, Nat Methods, 6, 737, 10.1038/nmeth.1368 · DOI
  36. Tomat, 2008, Organelle-specific zinc detection using Zinpyr-labeled fusion proteins in live cells, J Am Chem Soc, 130, 15776, 10.1021/ja806634e · DOI
  37. Helmchen, 2005, Deep tissue two-photon microscopy, Nat Methods, 2, 932, 10.1038/nmeth818 · DOI
  38. Kim, 2009, Two-photon probes for intracellular free metal ions, acidic vesicles, and lipid rafts in live tissues, Acc Chem Res, 42, 863, 10.1021/ar800185u · DOI
  39. Sumalekshmy, 2007, Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness, J Am Chem Soc, 129, 11888, 10.1021/ja073240o · DOI
  40. Chen, 2009, Two-photon fluorescent probes of biological Zn(II) derived from 7-hydroxyquinoline, Org Lett, 11, 4426, 10.1021/ol901787w · DOI
  41. Kim, 2008, Two-photon fluorescent probes for intracellular free zinc ions in living tissue, Angew Chem Int Ed, 47, 5167, 10.1002/anie.200800929 · DOI
  42. Levitt, 2009, Fluorescence lifetime and polarization-resolved imaging in cell biology, Curr Opin Biotechnol, 20, 28, 10.1016/j.copbio.2009.01.004 · DOI
  43. Felton, 2008, A wavelength and lifetime responsive cryptate-containing fluorescent probe for zinc ions in water, Chem Commun, 6185, 10.1039/b813775g · DOI
  44. Major, 2007, The synthesis and in vitro testing of a zinc-activated MRI contrast agent, Proc Natl Acad Sci U S A, 104, 13881, 10.1073/pnas.0706247104 · DOI
  45. Zhang, 2007, Water-soluble porphyrins as a dual-function molecular imaging platform for MRI and fluorescence zinc sensing, Proc Natl Acad Sci U S A, 104, 10780, 10.1073/pnas.0702393104 · DOI
  46. Esqueda, 2009, A new gadolinium-based MRI zinc sensor, J Am Chem Soc, 131, 11387, 10.1021/ja901875v · DOI
  47. Wong, 2009, Solution and fluorescence properties of symmetric dipicolylamine-containing dichlorofluorescein-based Zn(II) sensors, J Am Chem Soc, 131, 7142, 10.1021/ja900980u · DOI
  48. Major, 2008, Mechanisms of Zn(II)-activated magnetic resonance imaging agents, Inorg Chem, 47, 10788, 10.1021/ic801458u · DOI
  49. Cody, 2008, Differential tuning of the electron transfer parameters in 1,3,5-triarylpyrazolines: a rational design approach for optimizing the contrast ratio of fluorescent probes, J Am Chem Soc, 130, 13023, 10.1021/ja803074y · DOI
  50. Zhao, 2008, The zinc indicator FluoZin-3 is not perturbed significantly by physiological levels of calcium or magnesium, Cell Calcium, 44, 422, 10.1016/j.ceca.2008.01.006 · DOI
  51. Zhao, 2009, The interaction of biological and noxious transition metals with the zinc probes FluoZin-3 and Newport Green, Anal Biochem, 384, 34, 10.1016/j.ab.2008.09.019 · DOI
  52. Frederickson, 2006, Synaptic release of zinc from brain slices: factors governing release, imaging, and accurate calculation of concentration, J Neurosci Methods, 154, 19, 10.1016/j.jneumeth.2005.11.014 · DOI

Cited by

Sorted by citations

See all 217

Related

Metadata

Authors
Tomat, E., & Lippard, S. J.
Affiliations
Massachusetts Institute of Technology (US)
Corresponding author
Lippard, Stephen J
ORCID
Venue
Current Opinion in Chemical Biology
Publisher
Elsevier BV
Published
2010-04
Volume
14
Issue
2
Pages
225-230
ISSN
1367-5931 (ISSN-L)
DOI
10.1016/j.cbpa.2009.12.010
PMC
PMC2847655
OpenAlex
W1567645807
Cited by
217
References
0
Citations by year
2026: 3 · 2025: 6 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 3 · 2019: 10 · 2018: 12 · 2017: 11 · 2016: 14 · 2015: 14 · 2014: 31 · 2013: 32 · 2012: 34
FWCI
2.67
Type
journal article
Language
en
Open access
Licence
Locations
  • Current Opinion in Chemical Biology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc-sa)
Funders
  • National Institutes of Health (5r01gm065519-16)
  • National Institute of General Medical Sciences (R01‐GM065519, GM065519)
Topics
  • Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
Keywords
zinc imaging, zinc regulation, fluorescent probes, live-cell imaging, chemical probes, real-time detection
MeSH terms
Animals, Fluorescent Dyes, Humans, Microscopy, Fluorescence, Spectrometry, Fluorescence, Zinc, Biosensing Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review