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Cited by 971Genomics and Chromatin DynamicsPlant Molecular Biology ResearchNuclear Structure and Function

H2A.Z-containing nucleosomes mediate the thermosensory response in Arabidopsis

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Kumar, S. Vinod, Wigge, Philip A.·2010·Cell
DOI 10.1016/j.cell.2009.11.006
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Abstract

Plants are highly sensitive to temperature and can perceive a difference of as little as 1 degrees C. How temperature is sensed and integrated in development is unknown. In a forward genetic screen in Arabidopsis, we have found that nucleosomes containing the alternative histone H2A.Z are essential to perceiving ambient temperature correctly. Genotypes deficient in incorporating H2A.Z into nucleosomes phenocopy warm grown plants, and show a striking constitutive warm temperature transcriptome. We show that nucleosomes containing H2A.Z display distinct responses to temperature in vivo, independently of transcription. Using purified nucleosomes, we are able to show that H2A.Z confers distinct DNA-unwrapping properties on nucleosomes, indicating a direct mechanism for the perception of temperature through DNA-nucleosome fluctuations. Our results show that H2A.Z-containing nucleosomes provide thermosensory information that is used to coordinate the ambient temperature transcriptome. We observe the same effect in budding yeast, indicating that this is an evolutionarily conserved mechanism.

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  • Arabidopsis thaliana demonstrates that a general mechanism involving the variant histone H2AZ88 mediates part of the short-term adaptation of plants to temperature changes.

    “The plant Arabidopsis thaliana demonstrates that a general mechanism involving the variant histone H2AZ88 mediates part of the short-term adaptation of plants to temperature changes [97].”

    From Revisiting Epigenetics Fundamentals and Its Biomedical Implications (2024) · cites this paper as [97]

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Cited by

Sorted by citations

Authors
Kumar, S. V., & Wigge, P. A.
Affiliations
John Innes Centre (GB)
Corresponding author
Wigge, Philip A.
ORCID
Venue
Cell
Publisher
Elsevier BV
Published
2010-01
Volume
140
Issue
1
Pages
136-147
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2009.11.006
OpenAlex
W2152105724
Cited by
971
References
1
Citations by year
2026: 40 · 2025: 53 · 2024: 58 · 2023: 60 · 2022: 60 · 2021: 71 · 2020: 77 · 2019: 64 · 2018: 58 · 2017: 49 · 2016: 62 · 2015: 61 · 2014: 62 · 2013: 59 · 2012: 45
FWCI
18.27
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
Biotechnology and Biological Sciences Research Council (BBS/E/J/00000610)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
H2A.Z, nucleosome, thermosensation, thermal sensation, Arabidopsis thaliana, DNA unwrapping, chromatin dynamics, forward genetic screen, Saccharomyces cerevisiae, temperature sensitivity
MeSH terms
Histones, Microfilament Proteins, Nucleosomes, Temperature, Arabidopsis, DNA, Plant, HSP70 Heat-Shock Proteins, Gene Expression Profiling, Arabidopsis Proteins

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